Aberrant Myosin 1b Expression Promotes Cell Migration and Lymph Node Metastasis of HNSCC

Abstract

Lymph node metastasis is the major clinicopathologic feature associated with poor prognosis in patients with head and neck squamous cell carcinoma (HNSCC). Here, web-based bioinformatics meta-analysis was performed to elucidate the molecular mechanism of lymph node metastasis of human HNSCC. Preferential upregulation of Myosin 1b (MYO1B) transcript in HNSCC datasets was identified. Myo1b mRNA was highly expressed in human HNSCC cells and patient tissue specimens compared with their normal counterparts as shown by quantitative PCR (qPCR) analyses. Immunohistochemistry (IHC)-detected Myo1b expression was significantly correlated with lymph node metastases in patients with oral cancer of the tongue. HNSCC with high expression of Myo1b and chemokine receptor 4 (CCR4), another metastasis-associated molecule, was strongly associated with lymph node metastasis. RNA interference (RNAi) of Myo1b in HNSCC cells, SAS and HSC4, significantly inhibited migratory and invasive abilities through decreased large protrusion formation of cell membranes. Finally, Myo1b knockdown in SAS cells significantly inhibited in vivo cervical lymph node metastases in a cervical lymph node metastatic mouse model system. Implications: Myo1b is functionally involved in lymph node metastasis of human HNSCC through enhanced cancer cell motility and is an attractive target for new diagnostic and therapeutic strategies for patients with HNSCC.

Molecular pathogenesis of esophageal squamous cell carcinoma: Identification of the antitumor effects of miR‑145‑3p on gene regulation

“…Importantly, antitumor miR-145-3p directly regulated expression of MYO1B in head and neck cells (20). A previous in vivo study demonstrated that downregulation of MYO1B inhibited cervical lymph node metastasis in head and neck cancer cells (49). These findings indicate that aberrantly expressed MYO1B is associated with cancer cell aggressiveness and metastasis.…”

Exosomes Derived from Human Primary and Metastatic Colorectal Cancer Cells Contribute to Functional Heterogeneity of Activated Fibroblasts by Reprogramming Their Proteome

“…Myosin 1b is a member of class I myosin that promotes invasion in cervical cancer, and lymph node metastasis of head neck squamous cell carcinoma . Myosin 1b promotes invasion through large protrusion formation of cell membranes . ROCK‐dependent myosin organization results in protease‐independent matrix remodeling during invasion …”

Onco-Golgi: Is Fragmentation a Gate to Cancer Progression?

“…In many cases, the upregulation of Golgi-associated myosin motors is associated with aggressive cancer. For instance, overexpression of Myosin 1b was described in head and neck squamous cell carcinoma [88], Myosin Va in colorectal cancer [89], and Myosin VI in prostate cancer [90]. The Myosin 18a directly interacts with Golgi phosphoprotein 3 (GOLPH3) and their link triggers Golgi dispersal [91].…”