Aberrant miR-181b-5p and miR-486-5p expression in serum and tissue of non-small cell lung cancer

Tian, Fei; Shen, Yanting; Chen, Zhenzhu; Li, Rui; Lu, Jiafeng; Ge, Qinyu

doi:10.1016/j.gene.2016.06.014

Cited by 59 publications

(51 citation statements)

References 36 publications

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“…Similar results have been recently reported. Tian et al (39) characterized the expression profiles of miRNAs in sera and in tissues collected from NSCLC patients and found that miR-181b-5p was upregulated in serum and tissue of SCC patients. In our study, we modulated the expression of miR-181b-5p in A549 cells and CD133 + /CD326 + cells and found that miR-181b-5p enhanced invasion and metastasis in vitro and in vivo .…”

Section: Discussionmentioning

confidence: 99%

miR-181b-5p mediates TGF-β1-induced epithelial-to-mesenchymal transition in non-small cell lung cancer stem-like cells derived from lung adenocarcinoma A549 cells

Han

Zhu

et al. 2017

International Journal of Oncology

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The ability of non-small cell lung cancer (NSCLC) cells to invade and metastasize is associated with epithelial-to-mesenchymal transition (EMT). The process of EMT is, at least in part, regulated by microRNAs. However, it is unknown whether microRNAs regulate EMT in cancer stem-like cells (CSLCs), or which microRNAs are involved. In the present study, we compared microRNA expression in A549 cells, TGF-β1-treated A549 cells, CSLCs characterized by the CD133+/CD326+ phenotype, and TGF-β1-treated CSLCs. We found that miR-181b-5p expression was upregulated by TGF-β1. Moreover, the overexpression of the miR-181b-5p in A549 cells and CD133+/CD326+ cells resulted in the down-regulation of the E-cadherin and increased invasion and metastasis in vitro and in vivo. Accordingly, the knockdown of miR-181b-5p partially restored E-cadherin expression. These results suggest that miR-181b-5p regulates TGF-β1-induced EMT by targeting E-cadherin not only in normal A549 cells but also in CD133+/CD326+ cells which have characteristics of CSLCs. Thus, miR-181b-5p represents a new therapeutic target in NSCLC.

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Section: Discussionmentioning

confidence: 99%

miR-181b-5p mediates TGF-β1-induced epithelial-to-mesenchymal transition in non-small cell lung cancer stem-like cells derived from lung adenocarcinoma A549 cells

Han

Zhu

et al. 2017

International Journal of Oncology

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“…In addition, our former study revealed that miR-181b-5p and miR-21-5p were upregulated and miR-486-5p was downregulated in nonsmall cell lung cancer (NSCLC) tissue and serum samples compared to the normal controls [23]. In the study, we intended to quantify these 3 miRNAs to determine whether their expression levels were also similar between plasma and exosome from NSCLC blood samples.…”

Section: Resultsmentioning

confidence: 99%

“…Plasma RNA, exosome RNA, and supernatant RNA were respectively pooled from 10 healthy samples for small RNA library preparation and Illumina Hiseq2500 sequencing as described in our previous study [23]. The obtained reads were mapped to the corresponding database.…”

Section: Methodsmentioning

confidence: 99%

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No Significant Difference between Plasma miRNAs and Plasma-Derived Exosomal miRNAs from Healthy People

Tian

Shen

Chen

et al. 2017

BioMed Research International

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Background MicroRNAs (miRNAs) are small noncoding RNAs that are involved in many biological regulation processes. Studies have reported that miRNAs are enriched in human plasma and plasma-derived exosome as novel diagnostic biomarkers. The aim of this study was to determine whether the miRNA expression levels are different between plasma and plasma-derived exosome. Methods We sequenced and quantified the miRNAs in plasma and exosome from healthy blood samples and validated three miRNAs in the two groups of lung cancer samples by qRT-PCR. Results The sequencing results showed that only several of miRNAs were differential, while the qRT-PCR further validated that most of them did not have the consistent differences. However, the levels of two upregulated miRNAs (miR-181b-5p and miR-21-5p) in lung cancer were significantly higher in exosomes than plasma. Conclusions To the best of our knowledge, this study is the first to compare the expression levels of miRNAs between plasma and exosome in healthy blood samples. Our data suggested that the miRNA levels were similar in the two parts of the healthy people, whereas the two onco-miRNAs were significantly enriched in the exosome of lung cancer patients.

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“…It is confirmed that CD326 + cells are the initial cell population of lung cancer and can be used as surface markers of CSCs . LncRNAs and miRNAs, which are key biomarkers for diagnosis and prognosis, and play a significant role in the development of lung cancer . However, there are few reports on the relationship between lncRNAs or miRNAs and LCSCs, which need further discussion and research.…”

Section: Discussionmentioning

confidence: 97%

Molecular characterization of lung cancer: A two‐miRNA prognostic signature based on cancer stem‐like cells related genes

Yan-ping

Yang

Shen

et al. 2019

J of Cellular Biochemistry

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Lung cancer is one of the deadliest cancers worldwide. To increase the survival rate of lung cancer, it is necessary to explore specific prognosis markers. More and more evidence finds that noncoding RNA is closely associated with the survival of lung cancer, and cancer stem cells (CSCs) also play a significant role in the progress of lung cancer. The objective of this study is to find CSLCs genes that affect the prognosis of lung cancer. The differential expression of long noncoding RNAs (lncRNAs), microRNAs (miRNAs), messenger RNAs (mRNAs) in the Cancer Genome Atlas (TCGA) database and differential expression data from microarray of CD326+ and CD326− A549 cell are intersected to identify stable and consistent expression genes (2 lncRNAs, 15 miRNAs, and 134 mRNAs). The intersection of lncRNAs and miRNAs is analyzed by univariate and multivariate Cox regression to obtained prognostic genes. Two miRNAs (miR‐30b‐5p and miR‐29c‐3p) are significantly correlated with the overall survival rate. Then using these two miRNAs to construct a risk score model as a prognosis signature of lung cancer. Subsequently, we analyzed the association between two miRNAs and clinical information of lung cancer patients, of which T stage, Neoplasm cancer and risk score (P < .05) can be used as independent prognostic indicators of lung cancer. Finally, target genes of 2 miRNAs and 134 mRNAs were annotated with Gene Ontology and analyzed with Kyoto Encyclopedia of Genes and Genomes pathway, and verified with the GEO database. In summary, this study illustrates the role of miRNAs in the promotion of lung cancer by CSCs, which is important to find molecular biomarkers of lung cancer.

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Aberrant miR-181b-5p and miR-486-5p expression in serum and tissue of non-small cell lung cancer

Cited by 59 publications

References 36 publications

miR-181b-5p mediates TGF-β1-induced epithelial-to-mesenchymal transition in non-small cell lung cancer stem-like cells derived from lung adenocarcinoma A549 cells

miR-181b-5p mediates TGF-β1-induced epithelial-to-mesenchymal transition in non-small cell lung cancer stem-like cells derived from lung adenocarcinoma A549 cells

No Significant Difference between Plasma miRNAs and Plasma-Derived Exosomal miRNAs from Healthy People

Molecular characterization of lung cancer: A two‐miRNA prognostic signature based on cancer stem‐like cells related genes

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