Aberrant Methylation of the SOX21-AS1 Promoter Region Promotes Gene Expression and Its Clinical Value in Cervical Cancer

Du, Peipei; Zhi, Yanfang; Wang, Ruijie; Wang, Yongdong; Li, Huanhuan; Zhang, Xiaoan; Cheng, Guangming; Li, Xiaofu

doi:10.1007/s43032-020-00335-y

Cited by 11 publications

(11 citation statements)

References 26 publications

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“…Accordingly, other sequences with 6.0, 4.0, and 2.0 methylated cytosines were named 75met_SEPT9, 50met_SEPT9, and 25met_SEPT9, respectively. Since hypermethylated BCAT1 and SOX21-AS were shown to be significant in cancer [ 35 , 36 ], they were chosen as interferents. The sequences are shown in Table S1 .…”

Section: Resultsmentioning

confidence: 99%

Magnetic Nanoparticle-Based Electrochemical Sensing Platform Using Ferrocene-Labelled Peptide Nucleic Acid for the Early Diagnosis of Colorectal Cancer

et al. 2022

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Diagnostic biomarkers based on epigenetic changes such as DNA methylation are promising tools for early cancer diagnosis. However, there are significant difficulties in directly and specifically detecting methylated DNA regions. Here, we report an electrochemical sensing system based on magnetic nanoparticles that enable a quantitative and selective analysis of the methylated septin9 (mSEPT9) gene, which is considered a diagnostic marker in early stage colorectal cancer (CRC). Methylation levels of SEPT9 in CRC samples were successfully followed by the selective recognition ability of a related peptide nucleic acid (PNA) after hybridization with DNA fragments in human patients’ serums and plasma (n = 10). Moreover, this system was also adapted into a point-of-care (POC) device for a one-step detection platform. The detection of mSEPT9 demonstrated a limit of detection (LOD) value of 0.37% and interference-free measurement in the presence of branched-chain amino acid transaminase 1 (BCAT1) and SRY box transcription factor 21 antisense divergent transcript 1 (SOX21-AS1). The currently proposed functional platform has substantial prospects in translational applications of early CRC detection.

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Section: Resultsmentioning

confidence: 99%

Magnetic Nanoparticle-Based Electrochemical Sensing Platform Using Ferrocene-Labelled Peptide Nucleic Acid for the Early Diagnosis of Colorectal Cancer

et al. 2022

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“…The lncRNA, SOX21-AS1, is hypomethylated in cervical cancer, can serve as a new biomarker for the diagnosis of cervical squamous cell carcinoma, and is a potential therapeutic target. Moreover, SOX21-AS1 is correlated with clinical stage of nephroblastoma and regulates cell proliferation, which is a likely prognostic marker of nephroblastoma ( Zhang et al, 2019 ; Du et al, 2021 ). UNC5B-AS1 is highly expressed in various tumors and promotes tumor proliferation, migration, and invasion ( Wang et al, 2019 ; Wang H. et al, 2020 ; Tan et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

An m6A/m5C/m1A/m7G-Related Long Non-coding RNA Signature to Predict Prognosis and Immune Features of Glioma

Shao

Liu

et al. 2022

Front. Genet.

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Background: Gliomas are the most common and fatal malignant type of tumor of the central nervous system. RNA post-transcriptional modifications, as a frontier and hotspot in the field of epigenetics, have attracted increased attention in recent years. Among such modifications, methylation is most abundant, and encompasses N6-methyladenosine (m6A), 5-methylcytosine (m5C), N1 methyladenosine (m1A), and 7-methylguanosine (m7G) methylation.Methods: RNA-sequencing data from healthy tissue and low-grade glioma samples were downloaded from of The Cancer Genome Atlas database along with clinical information and mutation data from glioblastoma tumor samples. Forty-nine m6A/m5C/m1A/m7G-related genes were identified and an m6A/m5C/m1A/m7G-lncRNA signature of co-expressed long non-coding RNAs selected. Least absolute shrinkage and selection operator Cox regression analysis was used to identify 12 m6A/m5C/m1A/m7G-related lncRNAs associated with the prognostic characteristics of glioma and their correlation with immune function and drug sensitivity analyzed. Furthermore, the Chinese Glioma Genome Atlas dataset was used for model validation.Results: A total of 12 m6A/m5C/m1A/m7G-related genes (AL080276.2, AC092111.1, SOX21-AS1, DNAJC9-AS1, AC025171.1, AL356019.2, AC017104.1, AC099850.3, UNC5B-AS1, AC006064.2, AC010319.4, and AC016822.1) were used to construct a survival and prognosis model, which had good independent prediction ability for patients with glioma. Patients were divided into low and high m6A/m5C/m1A/m7G-LS groups, the latter of which had poor prognosis. In addition, the m6A/m5C/m1A/m7G-LS enabled improved interpretation of the results of enrichment analysis, as well as informing immunotherapy response and drug sensitivity of patients with glioma in different subgroups.Conclusion: In this study we constructed an m6A/m5C/m1A/m7G-LS and established a nomogram model, which can accurately predict the prognosis of patients with glioma and provides direction toward promising immunotherapy strategies for the future.

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“…Accumulating evidence has indicated that lncRNAs may serve as diagnostic and prognostic biomarkers in different types of cancer ( 29 – 31 ). In addition, certain lncRNAs, such as lncRNA SOX21 antisense divergent transcript 1 ( 32 ) and lncRNA LIPE antisense RNA 1 (LIPE-AS1) ( 33 ), have been indicated to be of use as diagnostic and prognostic biomarkers for patients with CC. The present study also suggested that DLEU1 had the ability to screen patients with CC from normal controls.…”

Section: Discussionmentioning

confidence: 99%

Survival‑related DLEU1 is associated with HPV infection status and serves as a biomarker in HPV‑infected cervical cancer

Dong

Wang

et al. 2022

Mol Med Rep

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Human papillomavirus (HPV) is the most common risk factor for the occurrence of cervical cancer (cc). in recent years, the important roles of long non-coding rnas (lncrnas) in cc have emerged, but studies on the relationship between lncrnas and HPV-positive (HPV+) cc remain scarce. The present study aimed to investigate whether lncrna deleted in lymphocytic leukemia 1 (dleu1) is associated with HPV infection and explore the clinical significance of DLEU1 in HPV+ patients with cc. dleu1 expression was detected by reverse transcription-quantitative Pcr. The ability of dleu1 to screen patients with cc from controls and differentiate individuals with different HPV infection status was evaluated by receiver operating characteristic analysis. The association of dleu1 with the survival prognosis of patients with cc was assessed by Kaplan-Meier survival analysis and cox regression analysis. The rna interactome database was used to predict molecules interacting with dleu1. The results indicated that DLEU1 expression was significantly upregulated in CC tissues and cell lines, particularly in those that were HPV+. in addition, dleu1 had a high diagnostic value in discriminating patients with cc and differentiating between HPV+ and HPV-patients with cc, and had a certain ability to screen HPV+ controls. dleu1 was correlated with HPV infection in cc patients. Furthermore, dleu1 was indicated to be associated with survival prognosis in both total patients with cc and HPV+ patients with cc, and independently predict the prognosis of patients with cc. Most of the molecules interacting with dleu1 were micrornas. in conclusion, abnormal dleu1 expression is associated with HPV infection and may serve as a diagnostic and prognostic biomarker for HPV+ patients with cc.

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Aberrant Methylation of the SOX21-AS1 Promoter Region Promotes Gene Expression and Its Clinical Value in Cervical Cancer

Cited by 11 publications

References 26 publications

Magnetic Nanoparticle-Based Electrochemical Sensing Platform Using Ferrocene-Labelled Peptide Nucleic Acid for the Early Diagnosis of Colorectal Cancer

Magnetic Nanoparticle-Based Electrochemical Sensing Platform Using Ferrocene-Labelled Peptide Nucleic Acid for the Early Diagnosis of Colorectal Cancer

An m6A/m5C/m1A/m7G-Related Long Non-coding RNA Signature to Predict Prognosis and Immune Features of Glioma

Survival‑related DLEU1 is associated with HPV infection status and serves as a biomarker in HPV‑infected cervical cancer

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