2009
DOI: 10.1111/j.1600-0609.2008.01178.x
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Aberrant methylation of the death‐associated protein kinase 1 (DAPK1) CpG island in chronic myeloid leukemia

Abstract: The death-associated protein kinase 1 (DAPK1) gene is a candidate tumor suppressor (TSG) and the abnormal methylation of DAPK1 gene has been found in many carcinomas. The epigenetic changes of TSGs are now recognized as a mechanism contributing to the development of chronic myeloid leukemia (CML). To clarify the role of DAPK1 in CML, we examined the methylation status of DAPK1 in 49 patients with CML using methylation-specific polymerase chain reaction. The aberrant methylation of the DAPK1 gene was found in 2… Show more

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Cited by 43 publications
(40 citation statements)
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“…The death-associated protein kinase 1 (DAPK1) is part of a 5-member family of proapoptotic serine/threonine kinases that are ubiquitously expressed and are capable of inducing apoptosis. 49 DAPK1 hypermethylation has been specifically linked to pediatric leukemias carrying the MLL-AF4 oncoprotein, 32 the development of chronic lymphocytic leukemia, 50 progression of chronic myeloid leukemia to blast crisis, 33 and several solid tumors. 51,52 This study confirms the previous findings and also suggests that DAPK1 promoter hypermethylation may be a phenomenon of several different MLL translocations.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…The death-associated protein kinase 1 (DAPK1) is part of a 5-member family of proapoptotic serine/threonine kinases that are ubiquitously expressed and are capable of inducing apoptosis. 49 DAPK1 hypermethylation has been specifically linked to pediatric leukemias carrying the MLL-AF4 oncoprotein, 32 the development of chronic lymphocytic leukemia, 50 progression of chronic myeloid leukemia to blast crisis, 33 and several solid tumors. 51,52 This study confirms the previous findings and also suggests that DAPK1 promoter hypermethylation may be a phenomenon of several different MLL translocations.…”
Section: Discussionmentioning
confidence: 99%
“…PCR was carried out following the protocol written for methylation-specific PCR (MSP) by Licchesi and Herman. 31 MSP primers for DAPK1 CONTROL and p73 CONTROL , 32 DAPK1, 33 and HRK 34 were taken from the published literature. MSP primers for CCR6 were designed using the UCSC Genome Browser human assembly (March 2006).…”
Section: Mspmentioning
confidence: 99%
“…The reaction mixture (20 l) contained 10 ng of templates, 4 pmol of each primer, 4 nmol of dNTP, 1 unit of HotStarTaq DNA polymerase (QIAGEN GmbH, Hilden, Germany) and 2 ml of 10 £ GC-buVer. Primer sequences and PCR conditions for MSP have been reported in previous studies [8][9][10].…”
Section: Ampliwcation Of the Methylated Cpg Islandmentioning
confidence: 99%
“…For example, genes that are related to cell cycle regulation such as P16 INK4A and P15 INK4B are silenced in many cancers due to DNA methylation enabling uninhibited proliferation (e.g., [12]). Other genes often hypermethylated, and thus silenced, in cancer can be involved in: cell adhesion, such as E-cadherin and H-cadherin, enabling invasion and/or metastasis of the tumor (e.g., [13]); apoptosis, such as DAPK1, enabling resistance to cell death (e.g., [14]); and finally genes associated with DNA repair processes are also often hypermethylated in tumors, thus suggesting that epigenetic events may promote classical genetic alterations such as mutations.…”
Section: Doi: 107243/2052-6199-2-3mentioning
confidence: 99%