Aberrant Methylation-Mediated Silencing of lncRNA MEG3 Functions as a ceRNA in Esophageal Cancer

Zhao, Dong; Zhang, Aili; Liu, Shengnan; Lü, Fan; Guo, Yanli; Zhang, Guoqiang; Xu, Fenglou; Ya-bin, Shi; Shen, Supeng; Liang, Jia; Guo, Wei

doi:10.1158/1541-7786.mcr-16-0385

Cited by 110 publications

(103 citation statements)

References 34 publications

Supporting

Mentioning

100

Contrasting

Order By: Relevance

“…Recent research focusing on lncRNAs has improved our understanding of the complex regulatory mechanisms underlying tumorigenesis and metastasis in human cancers [10]. In ESCC, the dysregulation of some lncRNAs, such as MALAT1 [23,24], 91H [25], HOTAIR [26], ANRIL [27,28], and MEG3 [29,30], promotes or inhibits the progression and metastasis of tumors and is closely associated with poor survival [31]. These results confirm that lncRNAs are potential diagnostic and therapeutic biomarkers in ESCC.…”

Section: Discussionmentioning

confidence: 75%

Predictive Value of LINC01133 for Unfavorable Prognosis was Impacted by Alcohol in Esophageal Squamous Cell Carcinoma

Yang

Tien

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Considerable evidence indicates that long noncoding RNAs (lncRNAs) exert importantly regulatory functions during human cancer initiation and progression and are promising biotargets in the flight against cancer. Methods: In this study, we evaluated the role of the lncRNA LINC01133 in esophageal squamous cell carcinoma (ESCC). LINC01133 expression in ESCC was examined by quantitative real-time PCR. The correlations between LINC01133 expression and clinicopathological variables and survival were examined by the χ² test, Kaplan–Meier method, log-rank test, and univariate Cox regression analysis. Results: LINC01133 expression levels were frequently lower in ESCC tissues and cell lines than in paired normal tissues and an immortalized esophageal epithelial cell line, respectively. The expression of LINC01133 decreased in a TNM stage- and lifestyle-independent manner. LINC01133 was an independent protective factor and had an anti-tumor effect in the early stage of ESCC development. More importantly, we discovered that drinking status in our cohort impaired the predictive accuracy of LINC01133 for patients with ESCC. Furthermore, a new risk model combining LINC01133 expression, drinking status, and TNM stage provided better survival discrimination compared with three other predictors. Conclusions: Our data indicate that a loss of LINC01133 expression is a potential poor prognostic biomarker and therapeutic target for ESCC and provide additional prognostic information to improve the outcomes of ESCC patients.

show abstract

Section: Discussionmentioning

confidence: 75%

Predictive Value of LINC01133 for Unfavorable Prognosis was Impacted by Alcohol in Esophageal Squamous Cell Carcinoma

Yang

Tien

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Maternally expressed gene 3 is an imprinted gene commonly expressed in several normal tissues, such as pituitary and cerebellum . However, it is usually down‐regulated in non–small‐cell lung cancer, breast cancer, hepatocellular carcinoma, gastric cancer, cervical cancer, oesophageal cancer, prostate cancer, nasopharyngeal carcinoma and gliomas, even lost in pituitary tumours, meningiomas and myelomas . The low expression of MEG3 in cancer cells and tissues is likely to be related to aberrant promoter hypermethylation .…”

Section: Discussionmentioning

confidence: 99%

LncRNA MEG3 inhibits cell proliferation and induces apoptosis in laryngeal cancer via miR‐23a/APAF‐1 axis

Zhang

Wang

et al. 2019

J Cellular Molecular Medi

View full text Add to dashboard Cite

Long non‐coding RNA (LncRNA) MEG3 serves a regulatory role in the progression of several types of cancer, but the role of MEG3 in laryngeal cancer is still unknown. The aim of this study was to explore the regulatory role and mechanism of MEG3 in laryngeal cancer. MEG3 expression in 50 laryngeal cancer tissue samples was detected by reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR). The effects of MEG3 overexpression on laryngeal cancer cells were investigated in vitro and in vivo. The mechanism of competitive endogenous RNA (ceRNA) was validated through luciferase reporter assay, RT‐qPCR and Western blotting. MEG3 was down‐regulated in laryngeal cancer tissues, and the low MEG3 expression was associated with advanced clinical stage. Additionally, MEG3 overexpression inhibited the proliferation and induced the apoptosis of laryngeal cancer cells in vitro and in vivo. Particularly, MEG3 bound to miR‐23a specifically and a reciprocal negative regulation existed between miR‐23a and MEG3. Moreover, MEG3 up‐regulated apoptotic protease activating factor‐1 (APAF‐1), a known miR‐23a's target, thereby leading to the activation of caspase‐9 and caspase‐3. Meanwhile, these activated effects were rescued by miR‐23a overexpression. In conclusion, the present study demonstrated that MEG3 functions as a novel tumour suppressive LncRNA in laryngeal cancer for the first time. Furthermore, MEG3 may act as a ceRNA to regulate APAF‐1 expression by competitive binding to miR‐23a, thereby regulating the progression of laryngeal cancer.

show abstract

“…[10][11][12] Previous studies have showed that LINC00319 is involved in the occurrence and development of lung cancer. [10][11][12] Previous studies have showed that LINC00319 is involved in the occurrence and development of lung cancer.…”

Section: Discussionmentioning

confidence: 99%

Upregulation of LINC00319 indicates a poor prognosis and promotes cell proliferation and invasion in cutaneous squamous cell carcinoma

Liao

Duan

et al. 2018

J of Cellular Biochemistry

View full text Add to dashboard Cite

Cutaneous squamous cell carcinoma (CSCC), an epidermal keratinocyte-derived skin tumor, is one of the most leading causes of cancer-associated morbidity and mortality worldwide. Long noncoding RNAs have emerged as key regulators of tumor development and progression. Recent studies have identified LINC00319, a long intergenic noncoding RNA, as an oncogene in lung cancer. However, the biological role of LINC00319 in CSCC remains largely unknown. The current study aimed to explore the role of LINC00319 in CSCC and uncover the molecular mechanisms. In current study, we found that LINC00319 was significantly upregulated in both CSCC tissues and cell lines. Besides, the χ test showed that increased expression of LINC00319 was associated with larger tumor size, advanced TNM stage, and lymphovascular invasion. Gain-of-function and loss-of-function approaches were applied to investigate the effects of LINC00319 on CSCC cells. Functional studies demonstrated that LINC00319 promoted CSCC cell proliferation, accelerated cell cycle progression, facilitated cell migration and invasion, and inhibited cell apoptosis. Mechanistic studies revealed that LINC00319 exerts its oncogenic functions in CSCC via miR-1207-5p-mediated regulation of cyclin-dependent kinase 3. Taken together, upregulation of LINC00319 implies a potential link with poor prognosis and reflects CSCC progression. Collectively, this study may provide some evidence for LINC00319 as a candidate target in CSCC treatment.

show abstract

Aberrant Methylation-Mediated Silencing of lncRNA MEG3 Functions as a ceRNA in Esophageal Cancer

Cited by 110 publications

References 34 publications

Predictive Value of LINC01133 for Unfavorable Prognosis was Impacted by Alcohol in Esophageal Squamous Cell Carcinoma

Predictive Value of LINC01133 for Unfavorable Prognosis was Impacted by Alcohol in Esophageal Squamous Cell Carcinoma

LncRNA MEG3 inhibits cell proliferation and induces apoptosis in laryngeal cancer via miR‐23a/APAF‐1 axis

Upregulation of LINC00319 indicates a poor prognosis and promotes cell proliferation and invasion in cutaneous squamous cell carcinoma

Contact Info

Product

Resources

About