Aberrant keratin 7 and 20 expression in triple-negative carcinoma of the breast

Kuroda, Hajime; Imai, Yasuo; Yamagishi, Hidetsugu; Ueda, Yoshihiko; Kuroso, K; Oishi, Yoko; Ohashi, Hitoshi; Yamashita, Akinori; Yashiro, Yoshiko; Fukushima, Hisaki

doi:10.1016/j.anndiagpath.2015.10.007

Cited by 4 publications

(2 citation statements)

References 30 publications

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“…However, very few studies were focused on exhausted CD4 T cells in BC patients. Cytokeratins 7 (CK7) and 20 (CK20) were related to the pathological features of BC [ 8 ]. E-cadherin (E-Cad) and Ki-67 showed a significant relationship with BC prognosis [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Expression of PD-1 on CD4⁺Tumor-Infiltrating Lymphocytes in Tumor Microenvironment Associated with Pathological Characteristics of Breast Cancer

Zhao

Zhang

Shi

et al. 2018

Journal of Immunology Research

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Objective This study aimed to investigate the correlation of CD4+/PD-1+ or CD4+/PD-1− tumor-infiltrating lymphocytes with pathological characteristics in breast cancer patients. Methods A cross-sectional study consecutively recruited 133 patients with invasive ductal breast cancer. The expression of CD4, programmed cell death protein 1 (PD-1), CK7, CK20, E-cadherin, or Ki-67 was detected by immunohistochemistry. The associations between CD4+/PD-1+ or CD4+/PD-1− tumor-infiltrating lymphocytes and pathological characteristics were evaluated. Results Elderly patients intended to have a lower level of CD4+/PD-1− tumor-infiltrating lymphocytes (p < 0.05). Patients with positive E-cadherin expression had higher median cell counts of CD4+/PD-1− tumor-infiltrating lymphocytes than patients with negative E-cadherin expression (30/HPF versus 10/HPF, p < 0.05). Counts of CD4+/PD-1+ tumor-infiltrating lymphocytes had a significant correlation with Ki-67 index that the correlation coefficient was 0.29 (p = 0.001). Positive CK20 expression was related to a higher level of CD4+/PD-1− tumor-infiltrating lymphocytes than negative CK20 expression (73/HPF versus 30/HPF, p < 0.05). Conclusion CD4+/PD-1+ or CD4+/PD-1− tumor-infiltrating lymphocytes showed diverse association with pathological features of breast cancer. CD4+/PD-1+ tumor-infiltrating lymphocytes had a significant relationship with Ki-67 expression whereas CD4+/PD-1− tumor-infiltrating lymphocytes had a significant relationship with E-cadherin expression. Further studies are warranted to explore the immunomodulatory effects of phenotypes of CD4+ T cell subsets in breast cancer.

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Section: Introductionmentioning

confidence: 99%

Expression of PD-1 on CD4⁺Tumor-Infiltrating Lymphocytes in Tumor Microenvironment Associated with Pathological Characteristics of Breast Cancer

Zhao

Zhang

Shi

et al. 2018

Journal of Immunology Research

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“…KRT7 also participates in cervical cancer invasion, metastasis and promotes the malignant progression of cancer (16). Several studies have reported an association between KRT7 dysregulation and clinical pathology or prognosis (15,17); however, to the best of our knowledge, the molecular mechanisms involving KRT7 in promoting malignant progression have not been determined. KLK4-7 serve key roles in regulating the expression of their associated genes and proteins in ovarian cancer (18).…”

Section: Introductionmentioning

confidence: 99%

KRT7 promotes epithelial‑mesenchymal transition in ovarian cancer via the TGF‑β/Smad2/3 signaling pathway

Liu

Wang

et al. 2020

Oncol Rep

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Keratin 7 (KRT7) is a member of the keratin gene family. KRT7 is abnormally expressed in various types of cancer and promotes the malignant progression of tumors. However, the role of KRT7 in ovarian cancer remains unclear. The present study aimed to validate the role of KRT7 in ovarian cancer progression. KRT7 expression levels in patients with ovarian cancer were analyzed using data obtained from the Human Protein Atlas and The Cancer Genome Atlas databases. KRT7 mRNA and protein expression levels were upregulated in ovarian cancer tissue compared with normal tissue. KRT7 expression was associated with the grading, staging and poor prognosis of ovarian cancer. The differentially expressed genes affected by KRT7 were primarily enriched in the functions of cell migration, cell adhesion and cell growth. In vitro studies, including a CCK8 assay, were used to detect cell proliferation. In addition, wound healing and transwell assays were performed to analyze cell migration. The results demonstrated that KRT7 overexpression was associated with increased proliferation, migration and epithelial-mesenchymal transition (EMT) of ovarian cancer cells, and the migration and EMT of ovarian cancers cells were decreased following knockdown with KRT7 small interfering RNA. In vivo , knockdown of KRT7 inhibited tumor growth of ovarian cancer. Furthermore, KRT7 regulated EMT in ovarian cancer via the TGF-β/Smad2/3 pathway, and regulated cell-matrix adhesion through integrin-β1-focal adhesion kinase signaling. These results suggest that KRT7 may be a potential molecular marker for prognosis prediction in patients with ovarian cancer.

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Cytokeratin 7 and cytokeratin 20 expression in cancer: A tissue microarray study on 15,424 cancers

Dum

Menz

Völkel

et al. 2022

Experimental and Molecular Pathology

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Aberrant keratin 7 and 20 expression in triple-negative carcinoma of the breast

Cited by 4 publications

References 30 publications

Expression of PD-1 on CD4⁺Tumor-Infiltrating Lymphocytes in Tumor Microenvironment Associated with Pathological Characteristics of Breast Cancer

Expression of PD-1 on CD4⁺Tumor-Infiltrating Lymphocytes in Tumor Microenvironment Associated with Pathological Characteristics of Breast Cancer

KRT7 promotes epithelial‑mesenchymal transition in ovarian cancer via the TGF‑β/Smad2/3 signaling pathway

Cytokeratin 7 and cytokeratin 20 expression in cancer: A tissue microarray study on 15,424 cancers

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Aberrant keratin 7 and 20 expression in triple-negative carcinoma of the breast

Cited by 4 publications

References 30 publications

Expression of PD-1 on CD4+Tumor-Infiltrating Lymphocytes in Tumor Microenvironment Associated with Pathological Characteristics of Breast Cancer

Expression of PD-1 on CD4+Tumor-Infiltrating Lymphocytes in Tumor Microenvironment Associated with Pathological Characteristics of Breast Cancer

KRT7 promotes epithelial‑mesenchymal transition in ovarian cancer via the TGF‑β/Smad2/3 signaling pathway

Cytokeratin 7 and cytokeratin 20 expression in cancer: A tissue microarray study on 15,424 cancers

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Expression of PD-1 on CD4⁺Tumor-Infiltrating Lymphocytes in Tumor Microenvironment Associated with Pathological Characteristics of Breast Cancer

Expression of PD-1 on CD4⁺Tumor-Infiltrating Lymphocytes in Tumor Microenvironment Associated with Pathological Characteristics of Breast Cancer