Aberrant ganglioside composition in glioblastoma multiforme and peritumoral tissue: A mass spectrometry characterization

Fabris, Dragana; Rožman, Marko; Sajko, Tomislav; Vukelić, Željka

doi:10.1016/j.biochi.2017.03.001

Cited by 30 publications

(45 citation statements)

References 80 publications

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“…The structures confirmed by tandem MS are marked by asterisk (*). The proposed structures correspond to putative sphingoid base/fatty acid combinations, considering the most ubiquitous ceramide compositions found previously in human brain by MS methods . d‐dihydroxylated sphingoid base; t‐trihydroxylated sphingoid base.…”

Section: Resultsmentioning

confidence: 56%

“…On the other side, in relation to human cerebellum, glycomics was focused mainly on the compositional and structural analysis of lipid‐linked glycans and sialoglycans, in particular the sialylated glycosphingolipids known as gangliosides. This option was guided by the major role played by gangliosides in the central nervous system (CNS), not only as markers in the early diagnosis or prognosis of CNS diseases, but also as potential therapeutic agents and molecular fingerprints whose composition, being brain region‐ and cell‐type specific, changes during brain development, maturation, and aging.…”

Section: Introductionmentioning

confidence: 99%

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Orbitrap mass spectrometry for monitoring the ganglioside pattern in human cerebellum development and aging

et al. 2020

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We have developed here a superior approach based on high-resolution (HR) mass spectrometry (MS) for monitoring the changes occurring with development and aging in the composition and structure of cerebellar gangliosidome. The experiments were focused on the comparative screening and structural analysis of gangliosides expressed in fetal and aged cerebellum by Orbitrap MS with nanoelectrospray ionization (nanoESI) in the negative ion mode. The employed ultrahigh-resolution MS platform allowed the discrimination, without the need of previous separation, of 159 ions corresponding to 120 distinct species in the native ganglioside mixtures from fetal and aged cerebellar biopsies, many more than detected before, when MS platforms of lower resolution were employed. A number of gangliosides, in particular polysialylated belonging to GT, GQ, GP, and GS classes, modified by O-fucosylation, O-acetylation, or CH 3 COO − were discovered here, for the first time in human cerebellum. These components, found differently expressed in fetal and aged tissues, indicated that the ganglioside profile in cerebellum is development stage-and agespecific. Following the fragmentation analysis by high-energy collision-induced dissociation (HCD) tandem MS (MS/MS), we have also observed that the intimate structure of certain compounds has not changed during the development and aging of the brain, an aspect which could open new directions in the investigation of ganglioside biomarkers in cerebellar tissue.

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Section: Resultsmentioning

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Orbitrap mass spectrometry for monitoring the ganglioside pattern in human cerebellum development and aging

et al. 2020

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“…For MS analyses, the extracted GG mixture dissolved in methanol up to 0.8 mM concentration was infused into a Bruker amaZon ETD ion trap system. Whereas predominantly C18 chains were found to characterize the NB and PT, shorter (C16) and longer (C22, C24), unsaturated (C24:1) and with odd number carbon atom (C17, C19) fatty acids residues were detected in GBS [110]. The most abundant GG in GBS (accounting for 53% of the total GG content) was GD3(d18:1/18:0), followed by GD3(d18:1/24:0) that was exclusively detected in GBS tissue.…”

Section: Compositions Of Ggs Isolated From Human Gliosarcoma Specimenmentioning

confidence: 99%

“…A diminished amount of complex GGs in tumors is connected to the dedifferentiation processes, reflecting the GG pattern alteration in ontogenesis during differentiation of nervous tissue: an increased content of complex GGs and a reduced volume of simple GGs [110,111].…”

Section: Tof Ms Applications To Biomarkers Of Human Glioblastomamentioning

confidence: 99%

“…In order to reveal the role of GG as tumor-associated antigens, a detailed depiction of native GG mixtures from normal human brain (NB), GBM and corresponding peritumoral tissue [119], was conducted recently by HPTLC and MS [110]. Particular attention was paid to the GG expression in PT, since the biochemical processes occurring within this area are believed to be responsible for aggressive infiltration of tumor cells into the surrounding brain tissue and therefore for high incidence of GBM recidivism and poor prognosis of GBM [110,120].…”

Section: Compositions Of Ggs Isolated From Human Gliosarcoma Specimenmentioning

confidence: 99%

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Mass Spectrometry for Cancer Biomarkers

Albulescu¹,

Petrescu²,

Sârbu³

et al. 2019

Proteomics Technologies and Applications

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Cancer is considered as the second cause of morbidity and also mortality, after cardiovascular diseases. Despite the immense progress in efficacious biomarkers made in the present time, there are very few of them that can timely detect cancers or that can predict treatment outcomes or stratify patients according to their response to the treatment. Among other modern instruments involved in the research of this disease, proteomics emerged strongly, since it analyzes the "molecular effectors." Although it has some setbacks (like the lack of amplification of the signal), it is however one of the best means of investigating the presence and causes and predicting the evolution patterns of the disease. This chapter describes briefly pre-analytical (pre-MS steps), the main concepts, and the MS equipment used for such applications, followed by the presentation of several proteomic applications in melanoma, glioblastoma, pancreatic cancer, and colon cancer.

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Modern techniques for separation, mass spectrometric detection, and characterization of glycolipids

Sârbu

Zamfir

2021

Carbohydrate Analysis by Modern Liquid Phase Separation Techniques

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Aberrant ganglioside composition in glioblastoma multiforme and peritumoral tissue: A mass spectrometry characterization

Cited by 30 publications

References 80 publications

Orbitrap mass spectrometry for monitoring the ganglioside pattern in human cerebellum development and aging

Orbitrap mass spectrometry for monitoring the ganglioside pattern in human cerebellum development and aging

Mass Spectrometry for Cancer Biomarkers

Modern techniques for separation, mass spectrometric detection, and characterization of glycolipids

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