Aberrant Expression Profile of Long Noncoding RNA in Human Sinonasal Squamous Cell Carcinoma by Microarray Analysis

Abstract

Objectives. This study aimed to identify aberrantly expressed long noncoding RNAs (lncRNAs) profile of sinonasal squamous cell carcinoma (SSCC) and explore their potential functions. Methods. We investigated lncRNA and mRNA expression in SSCC and paired adjacent noncancerous tissues obtained from 6 patients with microarrays. Gene ontology (GO) analysis and pathway analysis were utilized to investigate the gene function. Gene signal-network and lncRNA-mRNA network were depicted. Quantitative real-time polymerase chain reaction (qRT-PCR) was utilized to validate 5 lncRNAs in a second set of paired SSCC and adjacent noncancerous tissues obtained from 22 additional patients. Results. We identified significantly differentially expressed lncRNAs (n = 3146) and mRNAs (n = 2208) in SSCC relative to noncancerous tissues. The GO annotation indicated that there are some core gene products that may be attributed to the progress of SSCC. The pathway analysis identified many pathways associated with cancer. The results of lncRNA-mRNA network and gene signal-network implied some core lncRNAs/mRNAs might play important roles in SSCC pathogenesis. The results of qRT-PCR showed that all of the 5 lncRNAs were differentially expressed and consistent with the microarray results. Conclusion. Our study is the first screening and analysis of lncRNAs expression profile in SSCC and may offer new insights into pathogenesis of this disease.

A transcriptome profile in gallbladder cancer based on annotation analysis of microarray studies

“…Nevertheless, efforts have been put into elucidating the pathophysiological mechanisms leading to the development of gallbladder cancer, however, most of these mechanisms remain unknown. Different from other microarray studies, in the present study, the microarrays were comprehensively analyzed (17)(18)(19). Firstly, eight differentially expressed upregulated genes were found, which included LOC101928168, HMGCS2, SCGN, CHN2, XK, MUC6, PLPP5 and HSPH1 both in cholesterol polyps and gallbladder adenoma from gallbladder walls.…”

Down-regulating NEAT1 inhibited the viability and vasculogenic mimicry formation of sinonasal squamous cell carcinoma cells via miR-195-5p/VEGFA axis

“…Multiple studies have suggested that lncRNAs might be implicated in sinonasal malignancies [ 22 ]. As an architectural component of nuclear paraspeckles, lncRNA NEAT1 has been found implicated in many human malignancies [ 23 , 24 ].…”