Aberrant expression of UCA1 in gastric cancer and its clinical significance

Zheng, Qi; Wu, Feng; Dai, Weiwei; Zheng, Dingcheng; Zheng, Chao; Ye, H.; Zhou, Bin; Chen, J.-J.; Chen, P.

doi:10.1007/s12094-015-1290-2

Cited by 116 publications

(85 citation statements)

References 34 publications

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“…The ceRNA network we built reveals an unknown ceRNA regulatory network in GC. Recent study also identified that lncRNA interactions with miRNA and mRNA might act as potential diagnosis and prognosis biomarkers in cancer, such as H19, PVT1, HOTAIR, UCA1 and TINCR (7,(48)(49)(50)(51). In our ceRNA network, we also found these key lncRNAs.…”

Section: Discussionsupporting

confidence: 57%

Integrated analysis of long non-coding RNA competing interactions reveals the potential role in progression of human gastric cancer

Liang

Yao

et al. 2016

International Journal of Oncology

110

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Abstract. Abnormal expression of long non-coding RNAs (lncRNAs) have been shown to play an important role in tumor biology. The Cancer Genome Atlas (TCGA) platform is a large sample sequencing database of lncRNAs, and further analysis of the associations between these data and patients' clinical related information can provide new approaches to find the functions of lncRNA. In the present study, 361 RNA sequencing profiles of gastric cancer (GC) patients were selected from TCGA. Then, we constructed the lncRNA-miRNA-mRNA competitive endogenous RNA (ceRNA) network of GC. There were 25 GC specific lncRNAs (fold change >2, p<0.05) identified, 19 of them were included in ceRNA network. Subsequently, we selected these 19 key lncRNAs and analyzed the correlations with clinical features and overall survival, 14 of them were discriminatively expressed with tumor size, tumor grade, TNM stage and lymphatic metastasis (p<0.05). In addition, eight lncRNAs (RPLP0P2, FOXD2-AS1, H19, TINCR, SLC26A4-AS1, SMIM10L2A, SMIM10L2B and SNORD116-4) were found to be significantly associated with overall survival (log-rank p<0.05). Finally, two key lncRNAs HOTAIR and UCA1 were selected for validation of their expression levels in 82 newly diagnosed GC patients by qRT-PCR. Results showed that the fold changes between TCGA and qRT-PCR were 100% in agreement. In addition, we also found that HOTAIR was significantly correlated with tumor size and lymphatic metastasis (p<0.05), and UCA1 was significantly correlated with tumor size, TNM stage and lymphatic metastasis (p<0.05).The clinical relevance of the two lncRNAs and the bioinformatics analysis results were almost the same. Overall, our study showed the GC specific lncRNAs expression patterns and a ceRNA network in GC. Clinical features related to GC specific lncRNAs also suggested these lncRNAs are worthwhile for further study as novel candidate biomarkers for the clinical diagnosis of GC and potential indicators for prognosis. IntroductionNoncoding RNAs (ncRNAs) are transcripts that have no ability of coding proteins, which widely exit in high eukaryotics. According to their characteristics, ncRNAs can be divided into several subtypes including transfer RNA, small nucleolar RNA (snoRNA), ribosomal RNA (rRNA), microRNA (miRNA) and long non-coding RNA (lncRNA). The amount of the ncRNAs transcripts is >98% of the whole genome transcripts and have been suggested to represent transcriptional noise (1). However, more and more evidence indicates that transcriptional output of genome is far more complex than predicted, and suggests new paradigms of ncRNA regulation (2).Recent studies suggest that the ncRNAs may play important biological roles in transcriptional regulation, cellular development, formation of chromosome and RNA modification (3). Based on the transcript size, ncRNAs are grouped into small ncRNAs (<200 bp) and long ncRNAs (>200 bp, up to 100 kb). lncRNA is the functional end-product, and the level of lncRNA expression correlates directly with the level of the active molecule. Thus, ...

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Section: Discussionsupporting

confidence: 57%

Integrated analysis of long non-coding RNA competing interactions reveals the potential role in progression of human gastric cancer

Liang

Yao

et al. 2016

International Journal of Oncology

110

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“…UCA1 also had significantly increased concentrations in gastric cancer tissues and cell lines, and its expression correlated with both poorer differentiation and increased patient mortality. This suggests that UCA1 could be considered as an independent prognostic biomarker [25]. Combining this previously reported information with the results of our current study, it is highly likely that UCA1 might serve as a promising biomarker for the early detection and prognosis prediction of several types of cancers.…”

Section: The Effect Of Uca1 Inhibition On Cell Proliferation and Cellsupporting

confidence: 81%

UCA1, a long noncoding RNA, promotes the proliferation of CRC cells via p53/p21 signaling

Shen

Xiao

et al. 2016

Open Life Sciences

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Abstract:Objective: Recently, the role of long noncoding RNAs (lncRNAs) in human colorectal cancer (CRC) has been a subject of intense focus. We set out to determine the function of one lncRNA, termed urothelial carcinoma-associated 1 (UCA1) in CRC cell proliferation and its underlying mechanisms. Methods: Quantitative real-time PCR (qRT-PCR) was applied to detect the expression level of UCA1 in 50 pairs of CRC samples compared with non-tumor colon tissues. Cell growth was determined using the Cell Counting Kit-8 (CCK-8). Western blotting was carried out to analyze the related protein expression. Flow cytometry was done to evaluate cell apoptosis by UCA1 inhibition. Results: We found an increased expression of UCA1 in CRC samples. Knockdown of UCA1 in HCT116 cells induced a decrease in cell proliferation rate compared to control samples. This oncogenic activity may be enhanced through p53/ p21 signaling. Conclusion: Our results supported the hypothesis that upregulation of UCA1 contributes to the unlimited proliferation rate of CRC cells, at least partially through the negative regulation of p53/p21 signaling pathway. Finally, we found that UCA1 merely influenced CRC cell apoptosis.

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“…UCA1 is highly expressed in diverse cancer types, such as breast cancer (17), pancreatic cancer (18), gastric cancer (19), and colorectal cancer (20,21), suggesting that high expression of UCA1 might serve as a molecular marker for predicting metastasis and prognosis in these cancers (22). Several studies also demonstrated that upregulation of UCA1 is associated with EMT in breast cancer (23) and bladder cancer (24), but the underlying mechanism remains largely unknown.…”

Section: Introductionmentioning

confidence: 99%

LncRNA UCA1 promotes the invasion and EMT of bladder cancer cells by regulating the miR‑143/HMGB1 pathway

Luo

Chen

et al. 2017

Oncol Lett

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Abstract. The long non-coding RNA (lncRNA) urothelial carcinoma associated 1 (UCA1) is an oncogenic lncRNA in bladder cancer, and its upregulation is associated with enhanced cell invasion. However, the underlying mechanism remains to be elucidated. The present study demonstrated that UCA1 was positively associated with cell invasion ability and promoted epithelial-mesenchymal transition (EMT) of bladder cancer cells by inducing high mobility group box 1 (HMGB1). Furthermore, bioinformatics and luciferase reporter assays demonstrated binding sites of the tumor suppressive miR-143 within UCA1 and the 3'untranslated region of HMGB1. UCA1 negatively regulated miR-143 expression in a dose-dependent manner in bladder cancer cells. In addition, UCA1 and HMGB1 were upregulated and miR-143 was downregulated in bladder cancer specimens. Overall, the data suggested that UCA1 may promote the invasion and EMT of bladder cancer cells by regulating the miR-143/HMGB1 pathway, which exhibits an important regulatory role in the pathology of bladder cancer.

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Aberrant expression of UCA1 in gastric cancer and its clinical significance

Cited by 116 publications

References 34 publications

Integrated analysis of long non-coding RNA competing interactions reveals the potential role in progression of human gastric cancer

Integrated analysis of long non-coding RNA competing interactions reveals the potential role in progression of human gastric cancer

UCA1, a long noncoding RNA, promotes the proliferation of CRC cells via p53/p21 signaling

LncRNA UCA1 promotes the invasion and EMT of bladder cancer cells by regulating the miR‑143/HMGB1 pathway

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