Aberrant expression of miR-127, miR-21 and miR-16 in placentas of deceased cloned sheep

Ni, Wei; You, Shuang; Cao, Yang; Li, Cunyuan; Wei, Junchuang; Wang, Dawei; Qiao, Jun; Zhao, Xinxia; Hu, Shengwei; Quan, Renzhe

doi:10.1016/j.rvsc.2016.02.017

Cited by 10 publications

(9 citation statements)

References 36 publications

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“…Last but not least, recent data showed that FGR is associated with increased lung miR-126-3p levels, which is known to modulate the expression of angiogenic factor, in rats [54]. The importance of angiogenic regulatory pathways was also highlighted by the abnormal upregulated expression of miR-127, miR-21, and miR-16 in placentas of deceased cloned sheep with respect to controls [55]. These data are extremely fascinating, since miR-21 expression was associated with increased vascular resistance also in growth-restricted human pregnancies [8, 56].…”

Section: Discussionmentioning

confidence: 99%

Do miRNAs Play a Role in Fetal Growth Restriction? A Fresh Look to a Busy Corner

Chiofalo

Laganà

Vaiarelli

et al. 2017

BioMed Research International

107

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Placenta is the crucial organ for embryo and fetus development and plays a critical role in the development of fetal growth restriction (FGR). There are increasing evidences on the role of microRNAs (miRNAs) in a variety of pregnancy-related complications such as preeclampsia and FGR. More than 1880 miRNAs have been reported in humans and most of them are expressed in placenta. In this paper, we aimed to review the current evidence about the topic. According to retrieved data, controversial results about placental expression of miRNAs could be due (at least in part) to the different experimental methods used by different groups. Despite the fact that several authors have demonstrated a relatively easy and feasible detection of some miRNAs in maternal whole peripheral blood, costs of these tests should be reduced in order to increase cohorts and have stronger evidence. In this regard, we take the opportunity to solicit future studies on large cohort and adequate statistical power, in order to identify a panel of biomarkers on maternal peripheral blood for early diagnosis of FGR.

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Section: Discussionmentioning

confidence: 99%

Do miRNAs Play a Role in Fetal Growth Restriction? A Fresh Look to a Busy Corner

Chiofalo

Laganà

Vaiarelli

et al. 2017

BioMed Research International

107

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“…Additionally, an overall lower expression of RTL1 was also noted in cloned bovine fetuses than in fetuses derived by natural breeding or artificial insemination [66]. RTL1 expression was also found to be downregulated in placentas of deceased cloned sheep, delivered by cesarean section after 149-151 days [50]. The placentas of the deceased sheep were found to be heavier with a lower number of cotyledons than in the conventionally produced sheep.…”

Section: Discussionmentioning

confidence: 88%

“…We confirmed this finding using reverse transcription quantitative realtime polymerase chain reaction (RT-qPCR) in a larger sample set (Figure 1B). Next, we evaluated the expression patterns of miR-127, the microRNA that is known as one of the regulators of the expression of RTL1 [37,50]. There was an inverse relationship (r = −0.86, P < 0.05) between the expression pattern of miR-127 and RTL1 throughout the gestation (Figure 1B).…”

Section: Dynamics Of Rtl1 Expression In the Equine Chorioallantois Throughout Pregnancymentioning

confidence: 98%

Paternally expressed retrotransposon Gag-like 1 gene, RTL1, is one of the crucial elements for placental angiogenesis in horses

Dini

Carossino

Balasuriya

et al. 2021

Biology of Reproduction

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RTL1 (retrotransposon Gag like 1) is an essential gene in the development of the human and murine placenta. Several fetal and placental abnormalities such as intrauterine growth restriction (IUGR) and hydrops conditions have been associated with altered expression of this gene. However, the function of RTL1 has not been identified. RTL1 is located on a highly conserved region in eutherian mammals. Therefore, the genetic and molecular analysis in horses could hold important implications for other species, including humans. Here we demonstrated that RTL1 is paternally expressed and is localized within the endothelial cells of the equine (Equus caballus) chorioallantois. We developed an equine placental microvasculature primary cell culture and demonstrated that RTL1 knock-down leads to loss of the sprouting ability of these endothelial cells. We further demonstrated an association between abnormal expression of RTL1 and development of hydrallantois. Our data suggest that RTL1 may be essential for placental angiogenesis, and its abnormal expression can lead to placental insufficiency. This placental insufficiency could be the reason for IUGR and hydrops conditions reported in other species, including humans.

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“…In breast cancer patients, down-regulated miR-92a levels were found in tumor tissues and serum [ 28 ]. Dysregulated miR-127 in transgenic cloned sheep mediated the expression of retrotransposon-like gene 1(Rtl1), a key placental development gene that may cause developmental retardation [ 29 ]. MiR-694 was shown to be down-regulated in the liver of Lass II knockout mice and may be involved in the association between liver dysregulation and metabolism dysfunction [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

Effects of exposure to Streptococcus iniae on microRNA expression in the head kidney of genetically improved farmed tilapia (Oreochromis niloticus)

et al. 2017

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BackgroundGenetically improved farmed tilapia (GIFT, Oreochromis niloticus) are susceptible to infection by Streptococcus iniae when maintained in modern intensive culture systems. GIFT are commercially important fishes that are cultured widely in southern China. The role of microRNAs (miRNAs) in the regulatory response of GIFT to S. iniae infection has been underestimated and has not yet been well studied. Head kidney has an important immune function in teleost fishes. The main aim of this study was to determine the possible function of miRNAs in head kidney of S. iniae-infected GIFT. MiRNAs are small, non-coding RNAs that regulate gene expression by binding to the 3’-untranslated regions of their target mRNAs. MiRNAs are known to regulate immune-regulated signaling and inflammatory response pathways.ResultsHigh-throughput deep sequencing of two libraries (control group [CO] and infected group [IN]) of RNA extracted from GIFT head kidney tissues generated 12,089,630 (CO) and 12,624,975 (IN) clean reads. Bioinformatics analysis identified 1736 and 1729 conserved miRNAs and 164 and 165 novel miRNAs in the CO and IN libraries, respectively. Three miRNAs (miR-310-3p, miR-92, and miR-127) were found to be up-regulated and four miRNAs (miR-92d-3p, miR-375-5p, miR-146-3p, and miR-694) were found to be down-regulated in the S. iniae-infected GIFT. The expressions of these miRNAs were verified by quantitative real-time PCR. RNAhybrid and TargetScan were used to identify complementary miRNA and mRNA target sites, and the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases were used to annotate and predict potential downstream regulation of biological pathways. Seven target genes, which encode immune-related proteins (complement C3, cytidine deaminase, regulator of G-protein Rgs22, mitogen-activated protein kinase Mapk1, metabotropic glutamate receptorm GluR8, calcium-sensing receptor CaSR, and microtubule-associated protein Map1S) were predicted to play crucial roles in the GIFT response to S. iniae infection.Conclusions S. iniae outbreaks have hindered the development of the tilapia industry in China. Understanding the miRNA transcriptome of S. iniae-infected GIFT is important for exploring the immune responses regulated by miRNAs as well as for studying novel regulated networks to prevent and treat S. iniae infections in the future.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3591-z) contains supplementary material, which is available to authorized users.

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Aberrant expression of miR-127, miR-21 and miR-16 in placentas of deceased cloned sheep

Cited by 10 publications

References 36 publications

Do miRNAs Play a Role in Fetal Growth Restriction? A Fresh Look to a Busy Corner

Do miRNAs Play a Role in Fetal Growth Restriction? A Fresh Look to a Busy Corner

Paternally expressed retrotransposon Gag-like 1 gene, RTL1, is one of the crucial elements for placental angiogenesis in horses

Effects of exposure to Streptococcus iniae on microRNA expression in the head kidney of genetically improved farmed tilapia (Oreochromis niloticus)

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