Aberrant expression of LncRNA‐MIR31HG regulates cell migration and proliferation by affecting miR‐31 and miR‐31* in Hirschsprung's disease

Cai, Peng; Li, Hongxing; Huo, Weiwei; Zhu, Hongxia; Xu, Chao; Zang, Rujin; Lv, Wei; Xia, Yankai; Tang, Weibing

doi:10.1002/jcb.26830

Cited by 31 publications

(22 citation statements)

References 43 publications

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“…For example, lncRNA MIR100HG -derived miR-100 and miR-125b have been shown to participate in the resistance of colorectal cancer to cetuximab through Wnt/β-catenin signaling (15). MIR31HG , the host gene of miR-31 , has been shown to contribute to the pathogenesis of Hirschsprung’s disease through the MIR31HG - miR-31/31* - ITIH5 / PIK3CG pathway (16). MIR155HG -derived miR-155-5p and miR-155-3p have been shown to suppress the transcription and translation of protocadherin ( PCDH )9 and PCDH7 , thereby promoting glioma cell migration and invasion (17).…”

Section: Introductionmentioning

confidence: 99%

TGF-β-induced long non-coding RNA MIR155HG promotes the progression and EMT of laryngeal squamous cell carcinoma by regulating the miR-155-5p/SOX10 axis

et al. 2019

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Non-coding RNAs, particularly long non-coding RNAs (lncRNAs), play important roles in tumorigenesis. The miR-155 host gene ( MIR155HG ) lncRNA has been found to play a crucial role in tumor progression. However, the role of MIR155HG in laryngeal squamous cell carcinoma (LSCC) remains unclear. Thus, the aim of the present study was to explore the roles and underlying molecular mechanisms of action of MIR155HG and miR-155-5p in LSCC, in an effort to provide novel approaches for the antitumor therapy for LSCC. In the present study, the expression levels of miR-155-5p and MIR155HG were detected by reverse tran scription-quantitative polymerase chain reaction. In addition, the biological functions of MIR155HG and miR-155-5p on LSCC were evaluated in vitro by MTS assay, colony formation assay and Transwell assays, and in vivo by tumorigenesis assays. It was revealed that MIR155HG and miR-155-5p were significantly upregulated in LSCC tissues, and were associated with the TNM stage, pathological differentiation and lymph node metastasis. Moreover, the knockdown of MIR155HG and miR-155-5p inhibited the proliferation, migration and invasion of LSCC cells, whereas their overexpression exerted the opposite effects in vitro and MIR155HG overexpression promoted tumorigenesis in vivo . Furthermore, MIR155HG downregulation reduced the expression level of miR-155-5p . The inhibitory effect of MIR155HG knockdown on malignant behavior was abrogated by miR-155-5p overexpression. Bioinformatics analysis and luciferase reporter assay confirmed that miR-155-5p contributed to the progression of LSCC by directly binding to the 3′ untranslated region of SRY-related-HMG-box 10 ( SOX10 ). In addition, MIR155HG and miR-155-5p were upregulated by the induction of transforming growth factor-β ( TGF-β ) and promoted the expression of mesenchymal markers synergistically. On the whole, the findings of the present study indicate a novel role of MIR155HG in the TGF-β -induced EMT of LSCC cells by regulating EMT markers through the miR-155 / SOX10 axis. The MIR155HG / miR-155-5p / SOX10 axis plays an important role in promoting the progression of LSCC and may thus serve as a potential therapeutic target for LSCC treatment.

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Section: Introductionmentioning

confidence: 99%

TGF-β-induced long non-coding RNA MIR155HG promotes the progression and EMT of laryngeal squamous cell carcinoma by regulating the miR-155-5p/SOX10 axis

et al. 2019

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“…Because of the lack of enteric neurons, the patients suffer from abdominal enlargement and constipation, which even threaten their lives. Until now, several lncRNAs have been reported to be abnormally expressed in colon tissues of HSCR patients, which play an important regulatory role in the progression of HSCR ( 19 , 20 ). In this study, DRAIC was found to be highly expressed in colon tissues of HSCR patients, which was consistent with the results reported in a previous article ( 8 ).…”

Section: Discussionmentioning

confidence: 99%

LncRNA DRAIC regulates cell proliferation and migration by affecting the miR-34a-5p/ITGA6 signal axis in Hirschsprung’s disease

Sun

Wang

et al. 2021

ujms

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Background: Hirschsprung’s disease (HSCR) is a common defect in newborns, and studies have revealed that long non-coding RNA (lncRNA) is involved in the progression of HSCR. This research study aims to investigate the mechanism of downregulated RNA in cancer (DRAIC) on cell proliferation and migration in HSCR. Methods: Quantitative reverse transcription–polymerase chain reaction (qRT-PCR) was used to detect the expression of DRAIC in HSCR bowel stenosis tissues and normal colon tissues. Cell-counting kit-8 (CCK-8) and Transwell assays were employed to explore whether cellular functions change after overexpression or knockdown of the DRAIC in SH-SY5Y cells and human 293T cells. Protein expression levels were determined by Western blot analysis. RNA pull-down and dual-luciferase reporter assays were used to confirm the competitive relationship of DRAIC and integrin subunit alpha 6 (ITGA6) through their association with miR-34a-5p. Results: The lncRNA DRAIC was significantly increased in colon tissue from HSCR patients. The overexpression of DRAIC inhibited SH-SY5Y cell and human 293T cell proliferation and migration. Knockdown of DRAIC, however, promoted cell proliferation and migration. The RNA pull-down and dual-luciferase reporter assays have proven the competitive relationship between DRAIC and ITGA6 through their association with miR-34a-5p. Further rescue experiments have confirmed that DRAIC regulates cell proliferation and migration by affecting the miR-34a-5p/ITGA6 signal axis in HSCR. Conclusion: DRAIC promoted cell proliferation and migration by regulating the miR-34a-5p/ITGA6 signal axis in HSCR.

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“…Much evidence suggests a relationship between lncRNAs and HSCR, all based on the comparative expression assays of specific lncRNAs in bowel tissue from HSCR patients and controls [73][74][75][76][77][78]. In our study we have performed a differential qRT-PCR assay in the EPCs from HSCR patients and controls, which has allowed us to identify new lncRNAs as potential regulatory elements implicated in ENS development and HSCR onset.…”

Section: Discussionmentioning

confidence: 99%

Identification of New Potential LncRNA Biomarkers in Hirschsprung Disease

Torroglosa

Villalba‐Benito

Fernández

et al. 2020

IJMS

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Hirschsprung disease (HSCR) is a neurocristopathy defined by intestinal aganglionosis due to alterations during the development of the Enteric Nervous System (ENS). A wide spectrum of molecules involved in different signaling pathways and mechanisms have been described in HSCR onset. Among them, epigenetic mechanisms are gaining increasing relevance. In an effort to better understand the epigenetic basis of HSCR, we have performed an analysis for the identification of long non-coding RNAs (lncRNAs) by qRT-PCR in enteric precursor cells (EPCs) from controls and HSCR patients. We aimed to test the presence of a set lncRNAs among 84 lncRNAs in human EPCs, which were previously related with crucial cellular processes for ENS development, as well as to identify the possible differences between HSCR patients and controls. As a result, we have determined a set of lncRNAs with positive expression in human EPCs that were screened for mutations using the exome data from our cohort of HSCR patients to identify possible variants related to this pathology. Interestingly, we identified three lncRNAs with different levels of their transcripts (SOCS2-AS, MEG3 and NEAT1) between HSCR patients and controls. We propose such lncRNAs as possible regulatory elements implicated in the onset of HSCR as well as potential biomarkers of this pathology.

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Aberrant expression of LncRNA‐MIR31HG regulates cell migration and proliferation by affecting miR‐31 and miR‐31* in Hirschsprung's disease

Cited by 31 publications

References 43 publications

TGF-β-induced long non-coding RNA MIR155HG promotes the progression and EMT of laryngeal squamous cell carcinoma by regulating the miR-155-5p/SOX10 axis

TGF-β-induced long non-coding RNA MIR155HG promotes the progression and EMT of laryngeal squamous cell carcinoma by regulating the miR-155-5p/SOX10 axis

LncRNA DRAIC regulates cell proliferation and migration by affecting the miR-34a-5p/ITGA6 signal axis in Hirschsprung’s disease

Identification of New Potential LncRNA Biomarkers in Hirschsprung Disease

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