Aberrant expression of FcγRIIIA (CD16) contributes to the development of atherosclerosis

Huang, Ye; Yin, Hui-jun; Wang, Jingshang; Li, Qian; Wu, Chao; Chen, Ke-ji

doi:10.1016/j.gene.2012.02.006

Cited by 9 publications

(13 citation statements)

References 29 publications

(27 reference statements)

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“…Next, we investigated the role of CD14 in aortic macrophage precursor recruitment after only 4 days of AngII infusion, early during the course of AAA formation, using an in vitro assay . Avid binding of CD14 +/+ , but not CD14 −/− , macrophage precursors to aortic explant adventitia was detected (Figure E) in comparison with the luminal surface.…”

Section: Resultsmentioning

confidence: 99%

CD14 Directs Adventitial Macrophage Precursor Recruitment: Role in Early Abdominal Aortic Aneurysm Formation

Blomkalns

Gavrilă

Thomas

et al. 2013

JAHA

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BackgroundRecruitment of macrophage precursors to the adventitia plays a key role in the pathogenesis of abdominal aortic aneurysms (AAAs), but molecular mechanisms remain undefined. The innate immune signaling molecule CD14 was reported to be upregulated in adventitial macrophages in a murine model of AAA and in monocytes cocultured with aortic adventitial fibroblasts (AoAf) in vitro, concurrent with increased interleukin‐6 (IL‐6) expression. We hypothesized that CD14 plays a crucial role in adventitial macrophage precursor recruitment early during AAA formation.Methods and ResultsCD14−/− mice were resistant to AAA formation induced by 2 different AAA induction models: aortic elastase infusion and systemic angiotensin II (AngII) infusion. CD14 gene deletion led to reduced aortic macrophage infiltration and diminished elastin degradation. Adventitial monocyte binding to AngII‐infused aorta in vitro was dependent on CD14, and incubation of human acute monocytic leukemia cell line‐1 (THP‐1) monocytes with IL‐6 or conditioned medium from perivascular adipose tissue (PVAT) upregulated CD14 expression. Conditioned medium from AoAf and PVAT induced CD14‐dependent monocyte chemotaxis, which was potentiated by IL‐6. CD14 expression in aorta and plasma CD14 levels were increased in AAA patients compared with controls.ConclusionsThese findings link CD14 innate immune signaling via a novel IL‐6 amplification loop to adventitial macrophage precursor recruitment in the pathogenesis of AAA.

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Section: Resultsmentioning

confidence: 99%

CD14 Directs Adventitial Macrophage Precursor Recruitment: Role in Early Abdominal Aortic Aneurysm Formation

Blomkalns

Gavrilă

Thomas

et al. 2013

JAHA

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“…In contrast, the identification of FcgRIIA and FcgRIIIA as potential DCreg cell markers was unexpected because engagement of these activating FcRs by IgGs is known to mediate anaphylactic reactions, allergic reactions, or both. [52][53][54] Of note, we assessed the specificity of the aforementioned DCreg cell-and DC2-associated markers by (1) monitoring the expression of corresponding genes using qPCR on sorted leukocyte subsets (see Fig E8 in this article's Online Repository at www.jacionline.org) and (2) analyzing surface expression of those molecules in blood leukocytes by means of flow cytometry with multicolor staining with specific antibodies (see Fig E9 in this article's Online Repository at www.jacionline. org).…”

Section: Discussionmentioning

confidence: 99%

Changes in markers associated with dendritic cells driving the differentiation of either TH2 cells or regulatory T cells correlate with clinical benefit during allergen immunotherapy

Gueguen

Bouley

Moussu

et al. 2016

Journal of Allergy and Clinical Immunology

120

126

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“…The lack of an increase in non-classical monocytes could be due to blunted (cause unknown) differentiation of intermediate monocytes to non-classical monocytes 9, 31 . M-CSF and GM-CSF are thought to be the most potent inducers of differentiation towards the non-classical phenotype 12, 32, 33 . Better understanding of M-CSF and GM-CSF signalling in GCA and PMR patients may elucidate whether differentiation to non-classical monocytes is blunted in GCA and PMR.…”

Section: Discussionmentioning

confidence: 99%

Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis

Sleen

Wang

Geest

et al. 2017

Sci Rep

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Monocytes/macrophages are critical in systemic and local inflammation in giant cell arteritis (GCA) and possibly in clinically overlapping polymyalgia rheumatica (PMR). Therefore, we aimed to understand the contribution of monocyte subsets and the CX3CR1-CX3CL1 and CCR2-CCL2 migratory pathways, to the pathology of GCA. Peripheral blood monocytes were enumerated in samples from newly-diagnosed, untreated GCA and PMR patients and after prednisone-induced remission. The distribution of classical (CD14brightCD16neg) and the more pro-inflammatory, intermediate (CD14brightCD16+) and non-classical (CD14dimCD16+) monocyte subsets was analysed by flow cytometry. The phenotype of macrophages in temporal artery biopsies (TABs) from GCA patients was studied by immunohistochemistry and immunofluorescence. A clear monocytosis was seen in newly diagnosed GCA and PMR patients caused by elevated numbers of classical monocytes. Prednisone treatment suppressed numbers of non-classical monocytes. Both chemokine CX3CL1 and CCL2 were highly expressed in the TAB. Most macrophages in the TAB of GCA patients expressed non-classical monocyte markers CD16 and CX3CR1 whereas co-localisation of CD16 with classical monocyte marker CCR2 was infrequent. In conclusion, we report an altered distribution of monocyte subsets in both GCA and PMR patients. The majority of macrophages in TABs of GCA patients were CD68 + CD16 + CX3CR1 + CCR2− and thereby resembled the phenotype of non-classical monocytes.

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Aberrant expression of FcγRIIIA (CD16) contributes to the development of atherosclerosis

Cited by 9 publications

References 29 publications

CD14 Directs Adventitial Macrophage Precursor Recruitment: Role in Early Abdominal Aortic Aneurysm Formation

CD14 Directs Adventitial Macrophage Precursor Recruitment: Role in Early Abdominal Aortic Aneurysm Formation

Changes in markers associated with dendritic cells driving the differentiation of either TH2 cells or regulatory T cells correlate with clinical benefit during allergen immunotherapy

Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis

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