Aberrant expression of cell‐free nucleosomes in dermatomyositis/polymyositis

Abstract

Nucleosomes are typically located intracellularly, and extracellular levels of nucleosomes indicated the degree of cell death. We postulated aberrant nucleosomes expression in dermatomyositis (DM) and polymyositis (PM). To assess the aberrant expression of circulating nucleosomes in DM and polymyositis. 76 consecutive DM and PM patients from 1 September 2017 to 31 October 2018 were enrolled in this study, in addition to 20 healthy controls. The levels of circulating nucleosomes, with 16 kinds of detectable myositis-specific antibodies (MSAs) were detected in IIMs patients using enzyme-linked immunosorbent assay kit (ELISA). Receiver operating characteristic (ROC) curve analysis was performed for evaluating the ability of this candidate marker for detecting DM/PM. The results showed that the levels of circulating nucleosomes in DM/PM patients were significantly higher than that in normal individuals. Specifically, elevated levels of nucleosomes were associated with MDA5 Ab, ARS, and TIF1γAb. In addition, elevated levels of circulating nucleosomes correlated with skin eruption. This study has evidenced nucleosomes as a potential new biomarker of DM/PM. Aberrant nucleosomes expression occurs in myositis patients and the difference in nucleosomes expression between patients with and without skin eruption is statistically significant. A unique expression profile of elevated serum nucleosomes was detected in DM/PM-MDA5 Ab(+), ASS, and TIF1γAb(+).

Serum pro-inflammatory biomarkers associated with improvement in quality of life in pulmonary tuberculosis

“…Cell-free nucleosomes were measured using the Cell Death Detection ELISA Plus kit (Roche Diagnostics, Indianapolis, IN, US), which detects DNA and histones for specific mono- and oligonucleosomes detection, following the manufacturers’ instructions. Results are reported as a percentage of the positive control included in the kit as reported elsewhere ( 25 , 29 ).…”

Idiopathic inflammatory myopathies: one year in review 2021

“…Similarly, antiRo52 was found associated with RP-ILD and skin ulcers in anti-MDA5 patients (79). Serum markers were found able also to identify IIM subsets, as soluble interleukin-2R (sIL-2R) in discriminating DM from IMNM, particularly in case of active disease with ulcerative skin lesions (80), or neopterin in discriminating anti-MDA5 DM with severe ILD from other forms of IIMs (81); also, circulating nucleosomes, markers of cell apoptosis, were elevated in sera from IIMs patients compared to HCs, particularly in patients with anti-ARS, anti-MDA5 and anti-TIF1γ DM associated with skin involvement (82). Serum IL-17 levels were higher in ASS patients than in matched HCs, although apparently unrelated with the disease activity (83).…”

Oxidative Stress and Inflammatory Mediators in Exhaled Breath Condensate of Patients with Pulmonary Tuberculosis. A Pilot Study with a Biomarker Perspective

“…Quantification of cell-free nucleosomes was performed using the Cell Death Detection ELISA Plus kit (Roche Diagnostics, Indianapolis, IN, USA), which detects DNA and histones (antibodies from the mouse clones M-CA-33 and H11-4) for the specific detection of mono-and oligonucleosomes, following the manufacturers' instructions. Results are reported as a percentage of the positive control included in the kit as reported elsewhere [24].…”