Aberrant energy-linked reactions in mitochondria isolated from the livers of sheep infected with the liver fluke Fasciola hepatica

Rule, Christopher J.; Hanisch, M; Behm, Carolyn A.; Bygrave, Fyfe L.

doi:10.1016/0020-7519(91)90038-9

Cited by 7 publications

(3 citation statements)

References 3 publications

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“…In murine liver infected with the fluke, augmented activities of some enzymes participating in the Krebs cycle and the respiratory chain herald changes in the rate of oxidative reactions (Kolodziejczyk 1996). Uncoupling of oxidative phosphorylation, inhibition of respiratory reactions, and loss of oligomycin sensitivity by the F 1 F 0 -ATPase were demonstrated in mitochondria of rat hepatocytes (Rule et al 1989;Hanisch et al 1992;Lenton et al 1994). In rat fasciolosis, hepatocyte mitochondria accumulate free fatty acids and are depleted of phospholipids resulting in loss of membrane integrity (Lenton et al 1995) and other structural and functional changes.…”

Section: Introductionmentioning

confidence: 96%

Antioxidant potential of rat liver in experimental infection with Fasciola hepatica

2005

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The aim of this paper is to assess the antioxidant properties of rat liver in the course of acute and chronic fasciolosis. Wistar rats were infected per os with 30 metacercariae of Fasciola hepatica. Liver activities of antioxidant enzymes and concentrations of non-enzymatic antioxidants were determined at 4, 7, and 10 weeks post-infection. Activities of superoxide dismutase (Cu,Zn-SOD), glutathione peroxidase (GSH-Px), and glutathione reductase (GSSG-R) were decreased, catalase (CAT) activity was increased and non-enzymatic antioxidant concentrations (reduced glutathione, vitamins C, E and A) were reduced simultaneously with enhancement of lipid peroxidation processes as evidenced by increased levels of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE). Changes in the antioxidant abilities of the liver and in the phospholipid structure of the cell membrane were accompanied by rising activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) as markers of liver damage.

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Section: Introductionmentioning

confidence: 96%

Antioxidant potential of rat liver in experimental infection with Fasciola hepatica

2005

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“…Nevertheless, it is clear that the migration of the immature flukes produces a generalized liver dysfunction, as suggested by the concomitant alterations of the energy metabolism in hepatic mitochondria of ewes infected with Fasciola hepatica (Rule et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Effects of experimental subclinical fasciolosis on the feeding behaviour of sheep

Ferre¹,

González‐Gallego²,

Rojo-Vázquez³

et al. 1996

J. Anim. Feed Sci.

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Ten female Churra breed sheep were experimentally infected with 25 Fasciola hepatica metacercariae daily during six days. The animals were offered pelleted lucerne and water ad libitum. Feed and water intake was automatically recorded by computer monitoring of electronic balances. Liver damage caused by flukes was ascertained by measuring the activities of aspartate amino transferase, glutamate dehydrogenase and gamma-glutamyl transferase. Serum bile acids and glucose concentrations were also measured. Maximum plasma enzyme values were found at 10 weeks post infection. Plasma bile acid concentration increased from six to 13 weeks post infection. Glycaemia was decreased from the fourth week post infection until the end of the experiment. Total daily food intake decreased maximally between six and 12 weeks post infection. The number of meals per day and time spent eating and drinking decreased along the experimental infection. The rate of eating and drinking increased progressively. An association between anorexia and liver damage is suggested.

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“…The uncoupling of isolated mitochondria from parasitized host tissues has been documented for a variety of parasitic infections. These include Trichinella spiralis and Trichinella pseudospiralis in rat muscle [1], Anasakis simplex in fish muscle [2] and Fasciola hepatica in sheep and rat liver [3][4][5][6]. The latter study showed that not only were mitochondria that had been isolated from livers during the acute stage of the infection (2-6 weeks) uncoupled, but that there was an attenuation of respiration and a loss of oligomycin-sensitivity of the FoFl-ATPase at 3-4 weeks postinfection.…”

Section: Introductionmentioning

confidence: 99%

Aberrant mitochondrial respiration in the livers of rats infected with Fasciola hepatica: the role of elevated non-esterified fatty acids and altered phospholipid composition

Lenton

Behm

Bygrave

1995

Biochemical Journal

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The non-esterified fatty acid (NEFA) content and phospholipid composition of mitochondria isolated from the livers of Wistar rats infected with Fasciola hepatica were examined in relation to the aberrant mitochondrial respiration previously reported [Rule, Behm, and Bygrave (1989) Biochem. J. 260, 517-523]. At 2 weeks post-infection, elevated NEFA levels were associated with uncoupling of mitochondrial respiration that was reversible in vitro by the addition of BSA. State IV respiration rates showed a strong correlation with NEFA content. At 3 weeks post-infection, NEFA content had increased further and uncoupled mitochondria no longer showed any response to BSA. 31P-NMR analyses of cholate extracts of mitochondria from infected livers at 3 weeks post-infection revealed a marked loss of several major phospholipid species with a concomitant increase in catabolic products, particularly glycerophosphocholine and glycerophosphoethanolamine. Similar changes were observed in microsomal extracts. The NEFA content and phospholipid composition of mitochondria isolated from infected, athymic nude rats were not significantly different from uninfected, athymic rats. These findings suggest that uncoupling of liver mitochondria during infection with F. hepatica is the result of phospholipase activation mediated by the immune system of the host.

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Aberrant energy-linked reactions in mitochondria isolated from the livers of sheep infected with the liver fluke Fasciola hepatica

Cited by 7 publications

References 3 publications

Antioxidant potential of rat liver in experimental infection with Fasciola hepatica

Antioxidant potential of rat liver in experimental infection with Fasciola hepatica

Effects of experimental subclinical fasciolosis on the feeding behaviour of sheep

Aberrant mitochondrial respiration in the livers of rats infected with Fasciola hepatica: the role of elevated non-esterified fatty acids and altered phospholipid composition

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