: Gastric cancers GC with methylation of multiple CpG islands have a CpG island methylator phenotype CIMP and they can have different biological features. The aim of this study was to investigate the DNA methylation status of GCs and its association with their clinicopathological features. We evaluated the methylation status of four genes MINT1, MINT2, MINT25 and MINT31 in 105 primary GCs using bisul te-pyrosequencing analysis. We classi ed tumors as CIMPhigh CIMP-H , CIMP-low CIMP-L or CIMP-negative CIMP-N based on the methylation of MINT1, MINT2, MINT25, and MINT31. Overall, the prevalence of CIMP-H, CIMP-L and CIMP-N was 22% 23/105 , 52% 55/105 and 26% 27/105 , respectively. We observed a signi cant difference in tumor stage stages I-II vs. stages III-IV between CIMP-H and CIMP-N tumors P 0.0435 . No significant differences were observed in clinicopathological characteristics gender, age, location and tumor differentiation among the CIMP phenotypes. The prognoses of patients with a CIMP-H tumor is likely to be better than those with CIMP-L or CIMP-N tumors, but these differences are not statistically signi cant P 0.074 and P 0.200 . Our results suggest that CIMP may de ne a subgroup of GCs with distinct biological features.