Aberrant Behavior of Phosphofructokinase From Otosclerotic Stapedes: Biochemical Evidence and the Metabolic Consequences

Holdsworth, Charles E.; Endahl, Gerald L.; Soifer, Nathaniel; Richardson, K.E.

doi:10.1001/archotol.1973.00780020199012

Cited by 5 publications

(1 citation statement)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…14 Similarly, the effect of oxalate on LDH activity was determined by adding oxalate in a final concentration of 0.4 millimol to the standard LDH assay.…”

Section: Clinical Historymentioning

confidence: 99%

Comparative Biochemical Study of Otosclerosis and Osteogenesis Imperfecta

Holdsworth¹,

Endahl²,

Soifer³

et al. 1973

Archives of Otolaryngology - Head and Neck Surgery

Self Cite

View full text Add to dashboard Cite

“…14 Similarly, the effect of oxalate on LDH activity was determined by adding oxalate in a final concentration of 0.4 millimol to the standard LDH assay.…”

Section: Clinical Historymentioning

confidence: 99%

Comparative Biochemical Study of Otosclerosis and Osteogenesis Imperfecta

Holdsworth¹,

Endahl²,

Soifer³

et al. 1973

Archives of Otolaryngology - Head and Neck Surgery

Self Cite

View full text Add to dashboard Cite

A Contribution to the pathogenesis of otosclerosis

Mann¹,

Jonas

Riede

et al. 1980

Arch Otorhinolaryngol

View full text Add to dashboard Cite

In a histochemical and electron microscopical study the pathogenesis of otosclerosis was investigated. The morphological changes in the extra- and intracellular space indicated a complex metabolic disturbance of all tissue components. The observed chondrocytic chondrolysis stressed the role of the cartilage remnants in the otic capsule as an etiological factor. There was morphological evidence of a superimposed enzymatic defect.

show abstract

Study on normal and otosclerotic bone cell cultures: An advance in understanding the pathogenesis of otosclerosis

Maurizi¹,

Donti²,

Fanò³

et al. 1988

American Journal of Otolaryngology

View full text Add to dashboard Cite

The authors first reviewed the main theories concerning the pathogenesis of otoselerosis and studied the morphologic and functional characteristics of cell cultures derived from normal and otosclerotic bones. Light transmission and scanning electron microscopy did not permit definite identification of the cultured cells as predominantly osteoblasts, nor did these techniques show significant differences between cultured ceils derived from normal and pathologic bone. Functional tests of the cell cultures proved more interesting. First, the bony nature of the cultured cells was demonstrated by studying the intracellular 45Ca++ uptake after stimulation with calcitonin and dybutryl-cAMP. Second, cell cultures derived from otosclerotic bone behaved differently from those derived from normal bone. Their peak uptake of calcium appeared later, and post-stimulatory values were higher, suggesting that cells derived from otosclerotic bone store a greater quantity of 4SCa ++. Furthermore, after stimulation with calcitonin and propranolol, we observed an inhibition of the calcium uptake and decreased intracellular cAMP levels in normat bone cell cultures. In contrast, the cell cultures derived from otosclerotic bone exhibited an initial inhibition of calcium absorption followed by massive calcium penetration.The response of adenylate cyclase to the action of Mg + § Ca + +, and F-ions was evaluated in cultures derived from normal bone, otosclerotic bone, and normal skin fibroblasts. The resulting data show that activation due to Mg + § is much lower in cultured cells derived from otosclerotic bone than in those from either normal bone or skin fibroblasts. No significant differences were found after .Ca ++ inhibition in any of the cell cultures. Moreover, in cell cultures derived from normal bone, F-ions induced a strong activation that was lower than the levels observed in cultures of otosclerotic bone or in normal fibroblasts. We hypothesize that an alteration at the calcitonin receptor site is responsible for the difference in calcium uptake and cAMP levels observed in the cells derived from otosclerotic bone as compared to those cultured from normal cells.

show abstract

Aberrant Behavior of Phosphofructokinase From Otosclerotic Stapedes: Biochemical Evidence and the Metabolic Consequences

Cited by 5 publications

References 9 publications

Comparative Biochemical Study of Otosclerosis and Osteogenesis Imperfecta

Comparative Biochemical Study of Otosclerosis and Osteogenesis Imperfecta

A Contribution to the pathogenesis of otosclerosis

Study on normal and otosclerotic bone cell cultures: An advance in understanding the pathogenesis of otosclerosis

Contact Info

Product

Resources

About