Abelson antigen is expressed on hematopoietic spleen colony-forming cells from mice carrying the Av-2S virus sensitivity gene.

Self Cite

Lymphomas induced by the Abelson murine leukemia virus (A-MuLV) were examined for the expression of biochemical and biological markers associated with A-MuLV transformation before and after in vivo growth in genetically distinguishable host mice. Although all tumors and clonal lines derived from them initially expressed the A-MuLV-encoded gag fusion protein p160, they ceased synthesis of this molecule after several weeks of growth in vivo as ascites tumors. Transplanted clonal lines continued to express the alloantigenic marker H-2b and the isoenzyme marker Gpi-lb of the donor tumor cells, indicating that the cells were of donor and not host origin. Examination of cellular DNA obtained from p160-positive and derivative p160-negative lines indicated that p160-negative clones had lost A-MuLV-specific proviral sequences as detected by hybridization with several probes. Although the clonal lines no longer expressed p160, they retained their malignant phenotype and continued to express the Abelson antigen, a cell surface marker associated with A-MuLV lymphomagenesis. Continued expression of the A-MuLV genome was not required for maintenance of oncogenic potential under these conditions of in vivo tumor growth.

mentioning

confidence: 99%

Loss of viral gene expression and retention of tumorigenicity by Abelson lymphoma cells

Grunwald

Dale

Dudley

et al. 1982

Self Cite

“…ROTTER ET AL. Risser et al described an A-MuLV-related antigen that had a normal BALB/c counterpart in hematopoietic tissues of uninfected mice (16,17). We previously compared their data with data from our laboratory on the one known A-MuLV-encoded protein, P120, and its congeners and questioned whether their antigen is encoded by the A-MuLV genome (27).…”

mentioning

confidence: 99%

Abelson murine leukemia virus-induced tumors elicit antibodies against a host cell protein, P50

Rotter¹,

Witte²,

Coffman³

et al. 1980

166

“…However, endogenous murine leukemia virus expression is for the replication genes. The tissue specificity of endogenous AMV expression is similar to the expression ofAbelson antigen on murine hematopoietic cells (25).…”

Section: Np]mav-2 35smentioning

confidence: 88%

Expression of endogenous avian myeloblastosis virus information in different chicken cells

Chen¹

1980

Uninfected chicken cells were found to contain endogenous avian myeloblastosis virus (AMV)-specific information. Different tissues from chicken embryos and chickens expressed different amounts of the AMV-specific information. The endogenous AMV-related RNA was most abundant in bone marrow cells, which contained about 20 copies per cell. About 5 to 10 copies of AMV endogenous RNA per cell were found in embryonic yolk sac cells and bursa cells. The spleen, muscle, liver, and kidney cells of chickens and the fibroblasts of chicken embryos contained about two copies per cell. The amounts of AMV endogenous RNA in bone marrow, yolk sac, and bursa varied with age. From 19-day-old embryos to 2-week-old chickens, the bone marrow contained 20 copies of AMV RNA per cell. Bone marrow cells from 2-year-old chickens contained five copies per cell. Yolk sac cells of 10-day-old embryos and 1-day-old chickens were found to contain two copies per cell, whereas in 15-to 17-day-old embryos, these cells contained 5 to 10 copies. These results indicate that the level of endogenous AMV expression correlates with the development of granulopoiesis of the chicken hemopoietic system. The results of experiments on the thermostability of RNA-DNA hybrids indicated that the endogenous AMV RNA is closely related to viral AMV RNA. The expression of endogenous AMV information is independent of the activity of the chick helper factor. This endogenous AMV information is expressed as 20 to 21S RNA in both bone marrow and yolk sac cells.