Abdominal wall hernias—A local manifestation of systemically impaired quality of the extracellular matrix

Henriksen, Nadia A; Mortensen, Joachim Høg; Lorentzen, Lea; Ågren, Magnus S.; Bay‐Jensen, Anne‐Christine; Jørgensen, Lars N.; Karsdal, Morten A.

doi:10.1016/j.surg.2016.02.011

Cited by 17 publications

(9 citation statements)

References 38 publications

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“…Hernias have been suggested to be associated with a poor quality ECM, which systemically alters collagen turnover (36). ECM stability is directly associated with the levels of collagen synthesis and degradation (37).…”

Section: Discussionmentioning

confidence: 99%

Decreased collagen type�III synthesis in skin fibroblasts is�associated with parastomal hernia following colostomy

et al. 2019

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Parastomal hernia (PH) is a common complication following stoma formation. Abnormal collagen synthesis has been suggested to be involved in PH. The aim of the present study is to explore the effect and mechanism of the collagen synthesis on PH. Data from 157 patients with rectal cancer who received permanent colostomy were retrospectively collected and analyzed to identify the risk factors for PH. Primary culture of skin fibroblasts from patients with or without PH were performed. Cell viability, migration and invasion levels were detected by Cell Counting Kit-8, and wound healing and Transwell assays, respectively. Reverse transcription quantitative polymerase chain reaction and western blot analysis assays were performed to measure the gene and protein expression levels, respectively. The risk factors of sex, body mass index, aperture size and collagen expression were closely associated with the occurrence of PH. α1 (III) procollagen expression levels were significantly increased in patients with PH, while no marked difference in α1 (I) procollagen mRNA expression levels were observed in patients with or without PH. The viability and motility of fibroblasts from the patients with hernia were suppressed. The expression levels of matrix metalloproteinase (MMP)-2 and MMP-9 were decreased while the levels of collagen III and metalloproteinase inhibitor 1 (TIMP-1) were increased in the fibroblasts from the patients with PH. Silencing TIMP-1 expression promoted fibroblast migration and invasion and reversed the patterns of MMP-2, MMP-9 and collagen III expression in fibroblasts from the patients with PH. Decreased collagen III may inhibit the development of PH, potentially through decreases in TIMP-1 expression. Therefore, the results from the present study may provide a novel target for PH therapy.

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Section: Discussionmentioning

confidence: 99%

Decreased collagen type�III synthesis in skin fibroblasts is�associated with parastomal hernia following colostomy

et al. 2019

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“…The mean hernia size varied, and this could also have influenced the recurrence rate, as larger hernias usually have higher recurrence rates. Other patient factors such as smoking and healing may also have distorted the recurrence rate. Recurrences increase over time, and follow‐up must be long enough to assess the full effect of the repairs.…”

Section: Discussionmentioning

confidence: 99%

Systematic review and network meta-analysis of methods of mesh fixation during laparoscopic ventral hernia repair

Baker

Öberg

Andresen

et al. 2017

British Journal of Surgery

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“…PRO-C1/C1 M, PRO-C3/C3 M and PRO-C5/C5 M. 16,35 All assays were developed and produced by Nordic Bioscience A/S, Herlev, Denmark.…”

Section: Remodelling Of Types I Iii and V Collagenmentioning

confidence: 99%

“…To investigate the functional significance of the identified genetic variants in the collagen‐related genes, we aimed to measure the formation and degradation of the corresponding collagen subtypes, types I, III and V collagen, using highly specific biomarkers. The use of biomarkers of collagen remodelling have previously been investigated in a variety of conditions with potential implications for diagnostics and disease monitoring 16–18 . To our knowledge, collagen remodelling related to genetic variants in COL1A1 , COL3A1 , COL5A1 and COL5A2 has previously not been investigated in patients with unexplained bleeding symptoms.…”

Section: Introductionmentioning

confidence: 99%

“…The use of biomarkers of collagen remodelling have previously been investigated in a variety of conditions with potential implications for diagnostics and disease monitoring. [16][17][18] To our knowledge, collagen remodelling related to genetic variants in COL1A1, COL3A1, COL5A1 and COL5A2 has previously not been investigated in patients with unexplained bleeding symptoms.…”

Section: Introductionmentioning

confidence: 99%

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Collagen remodelling and plasma ascorbic acid levels in patients suspected of inherited bleeding disorders harbouring germline variants in collagen‐related genes

et al. 2020

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Introduction Variants in collagen‐related genes COL1A1, COL3A1, COL5A1 and COL5A2 are associated with Ehlers‐Danlos syndrome (EDS), a heterogeneous group of connective tissue disorders strongly associated with increased bleeding. Of patients with incompletely explained bleeding diathesis, a relatively high proportion were shown to harbour at least one heterozygous variant of unknown significance (VUS) in one of these genes, the vast majority without meeting the clinical criteria for EDS. Aim To investigate the functional consequences of the identified variants by assessing the formation and degradation of types I, III and V collagen, in addition to plasma levels of ascorbic acid (AA). Methods A total of 31 patients harbouring at least one heterozygous VUS in COL1A1, COL3A1, COL5A1 or COL5A2 and 20 healthy controls were assessed using monoclonal antibodies targeting neo‐epitopes specific for collagen formation and degradation. Plasma AA levels were measured in patients using high‐performance liquid chromatography. Results Serum levels of C5 M (degradation of type V collagen) were decreased in patients compared with healthy controls (p = .033). No significant differences were found in biomarkers for remodelling of types I and III collagen. A significant negative correlation between bleeding (ISTH‐BAT score) and plasma AA levels was shown (r = −.42; r2 = .17; p = .020). Suboptimal or marginally deficient AA status was found in 8/31 patients (26%). Conclusion Functional investigations of collagen remodelling were not able to identify any clear associations between the identified variants and increased bleeding. The negative correlation between plasma AA levels and ISTH‐BAT score motivates further investigations.

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Abdominal wall hernias—A local manifestation of systemically impaired quality of the extracellular matrix

Cited by 17 publications

References 38 publications

Decreased collagen type�III synthesis in skin fibroblasts is�associated with parastomal hernia following colostomy

Decreased collagen type�III synthesis in skin fibroblasts is�associated with parastomal hernia following colostomy

Systematic review and network meta-analysis of methods of mesh fixation during laparoscopic ventral hernia repair

Collagen remodelling and plasma ascorbic acid levels in patients suspected of inherited bleeding disorders harbouring germline variants in collagen‐related genes

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