Abdominal cysticercosis in a cynomolgus monkey

Tsubota, Kenjiro; Nakatsuji, Shunji; Matsumoto, Masahiro; Fujihira, Shiro; Yoshizawa, Katsuhiko; Okazaki, Yoshimasa; Murakami, Yûichi; Anagawa, Akiko; Oku, Yuzaburo; Oishi, Yuji

doi:10.1016/j.vetpar.2009.01.024

Cited by 12 publications

(7 citation statements)

References 5 publications

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“…The plot readily shows the high degree of nucleotide variation within and between genes amongst the aligned Taenia genomes for any given window of 300 bp (π ranges from 0.094 to 0.333), and the possibility that hitherto untested regions of mtDNAs may be of utility. GenBank holds relatively few mitochondrial markers for a few species of Taenia , used variously for population genetics and diagnostics, but include partial fragments of cox 1, cyt b [60,62], nad 1 [63], rrn S and atp 6 (unpublished data available on GenBank). Sliding window analysis (and computation of number of variable positions per unit length of gene), indicates that genes with high sequence variability included nad 6 (0.517), nad 5 (0.517), atp 6 (0.490), nad 3 (0.480) and nad 2 (0.479).…”

Section: Resultsmentioning

confidence: 99%

Complete mitochondrial genomes of Taenia multiceps, T. hydatigena and T. pisiformis: additional molecular markers for a tapeworm genus of human and animal health significance

et al. 2010

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BackgroundMitochondrial genomes provide a rich source of molecular variation of proven and widespread utility in molecular ecology, population genetics and evolutionary biology. The tapeworm genus Taenia includes a diversity of tapeworm parasites of significant human and veterinary importance. Here we add complete sequences of the mt genomes of T. multiceps, T. hydatigena and T. pisiformis, to a data set of 4 published mtDNAs in the same genus. Seven complete mt genomes of Taenia species are used to compare and contrast variation within and between genomes in the genus, to estimate a phylogeny for the genus, and to develop novel molecular markers as part of an extended mitochondrial toolkit.ResultsThe complete circular mtDNAs of T. multiceps, T. hydatigena and T. pisiformis were 13,693, 13,492 and 13,387 bp in size respectively, comprising the usual complement of flatworm genes. Start and stop codons of protein coding genes included those found commonly amongst other platyhelminth mt genomes, but the much rarer initiation codon GTT was inferred for the gene atp6 in T. pisiformis. Phylogenetic analysis of mtDNAs offered novel estimates of the interrelationships of Taenia. Sliding window analyses showed nad6, nad5, atp6, nad3 and nad2 are amongst the most variable of genes per unit length, with the highest peaks in nucleotide diversity found in nad5. New primer pairs capable of amplifying fragments of variable DNA in nad1, rrnS and nad5 genes were designed in silico and tested as possible alternatives to existing mitochondrial markers for Taenia.ConclusionsWith the availability of complete mtDNAs of 7 Taenia species, we have shown that analysis of amino acids provides a robust estimate of phylogeny for the genus that differs markedly from morphological estimates or those using partial genes; with implications for understanding the evolutionary radiation of important Taenia. Full alignment of the nucleotides of Taenia mtDNAs and sliding window analysis suggests numerous alternative gene regions are likely to capture greater nucleotide variation than those currently pursued as molecular markers. New PCR primers developed from a comparative mitogenomic analysis of Taenia species, extend the use of mitochondrial markers for molecular ecology, population genetics and diagnostics.

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Section: Resultsmentioning

confidence: 99%

Complete mitochondrial genomes of Taenia multiceps, T. hydatigena and T. pisiformis: additional molecular markers for a tapeworm genus of human and animal health significance

et al. 2010

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“…Altogether, to our knowledge, at least 10 different larval cestode infections have been reported in previous studies to affect members of more than 20 different lemur or monkey species (Table 3) [1, 2, 5, 6, 8, 9, 11–13, 17, 22–27, 29, 30, 34–36, 38, 39]. In most of these studies, mortality rates were high, showing that the control of these infections might be of critical importance in maintaining the well-being of NHPs living in parks.…”

Section: Discussionmentioning

confidence: 97%

Cestode infections in non-human primates suggest the existence of zoonotic cycles in the area surrounding the Strasbourg primatology center

et al. 2019

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Background: Several cases of infections due to Echinococcus multilocularis, Taenia martis and Taenia crassiceps were recently described in various species of captive non-human primates (NHPs) harbored in the Strasbourg Primate Center (SPC). Furthermore, one of the first cases of human cysticercosis due to T. martis was described in the Strasbourg region. These data suggest the existence of zoonotic cycles of tapeworm infections in the direct environment of the SPC. The aim of our study was to assess the prevalence of larval cestode infections among intermediate and definitive hosts in the close neighborhood of the center. We analyzed carnivore mammal fecal samples as well as rodent carcasses, collected inside or near the SPC, using PCR. Furthermore, we performed serology for Echinococcus spp. and Taenia spp. on NHP sera. Results: We found that 14.5% (95% CI [8.6; 20.4]) of 138 carnivore feces were positive for E. multilocularis-DNA, as well as 25% (95% CI [5.5; 57.2]) of 12 rodent carcasses, and 5.1% (95% CI [1.4; 8.7]) for T. martis or T. crassiceps. Of all NHPs tested, 10.1% (95% CI [3.8; 16.4]) were seropositive for Echinococcus spp. and 8.2% (95% CI [1.3; 15.1]) for Taenia spp. Conclusions: Our data support the existence of zoonotic cycles of larval cestode infections in the direct environment of the primatology center affecting NHPs harbored in the SPC, potentially threatening the human population living in this area. Since this zoonotic risk is borne by local wildlife, and given the severity of these infections, it seems necessary to put in place measures to protect captive NHPs, and further studies to better assess the risk to human populations.

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“…The T. hydatigena metacestode, commonly known as Cysticercus tenuicollis, uses a wide range of intermediate hosts, including ruminant and monogastric mammals (Abuladze, 1964;Tsubota et al, 2009). This metacestode has veterinary and economic importance due to its hepatic migration, especially in swine (Georgi, 1987;Trees et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

“…However, cysticerci of T. hydatigena have been found also in kidney, lung, brain, uterus, and others organs (Payan-Carreira et al, 2008;Tsubota et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

The taruca ( Hippocamelus antisensis ) and the red brocket deer ( Mazama americana ) as intermediate hosts of Taenia hydatigena in Peru, morphological and molecular evidence

Gómez-Puerta

Pacheco

Gonzales-Viera

et al. 2015

Veterinary Parasitology

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Abdominal cysticercosis in a cynomolgus monkey

Cited by 12 publications

References 5 publications

Complete mitochondrial genomes of Taenia multiceps, T. hydatigena and T. pisiformis: additional molecular markers for a tapeworm genus of human and animal health significance

Complete mitochondrial genomes of Taenia multiceps, T. hydatigena and T. pisiformis: additional molecular markers for a tapeworm genus of human and animal health significance

Cestode infections in non-human primates suggest the existence of zoonotic cycles in the area surrounding the Strasbourg primatology center

The taruca ( Hippocamelus antisensis ) and the red brocket deer ( Mazama americana ) as intermediate hosts of Taenia hydatigena in Peru, morphological and molecular evidence

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