2004
DOI: 10.1194/jlr.m300355-jlr200
|View full text |Cite
|
Sign up to set email alerts
|

ABCA1 and amphipathic apolipoproteins form high-affinity molecular complexes required for cholesterol efflux

Abstract: Apolipoproteins, such as apolipoprotein A-I (apoA-I), can stimulate cholesterol efflux from cells expressing the ATP binding cassette transporter A1 (ABCA1). The nature of the molecular interaction between these cholesterol acceptors and ABCA1 is controversial, and models suggesting a direct protein-protein interaction or indirect association have been proposed. To explore this issue, we performed competition binding and chemical cross-linking assays using six amphipathic plasma proteins and an 18 amino acid a… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3

Citation Types

10
103
0
4

Year Published

2004
2004
2014
2014

Publication Types

Select...
4
3

Relationship

0
7

Authors

Journals

citations
Cited by 135 publications
(119 citation statements)
references
References 20 publications
10
103
0
4
Order By: Relevance
“…Summary-Our results with apoA-I mutants support the two-step model for ABCA1-mediated lipid efflux proposed by Freeman, Zannis, and colleagues (24,25) while providing more insight into the lipidation process (see Fig. 8).…”
Section: Discussionsupporting
confidence: 62%
See 3 more Smart Citations
“…Summary-Our results with apoA-I mutants support the two-step model for ABCA1-mediated lipid efflux proposed by Freeman, Zannis, and colleagues (24,25) while providing more insight into the lipidation process (see Fig. 8).…”
Section: Discussionsupporting
confidence: 62%
“…The latter interaction is required for apoA-I to acquire and/or retain PL and FC to create nascent HDL particles. Recent evidence (24,25) indicates that apoA-I can form a high affinity complex with ABCA1 and that this is the first step in a two-step process of FC and PL efflux via ABCA1. The binding to ABCA1 is not very specific and apparently involves interactions of amphipathic helices with a hydrophobic site on the transporter (25).…”
Section: Discussionmentioning
confidence: 99%
See 2 more Smart Citations
“…We have previously elucidated the ABCA1 transporter's topology and characterized some of its interactions with apoA-I using this approach (11,12). In our ongoing effort to define the structure/function relationships that underlie the ABCA1 efflux mechanism, we focused on a truncation mutant that we had originally identified in a patient with Tangier disease (8).…”
mentioning
confidence: 99%