ABC transporters, neural stem cells and neurogenesis – a different perspective

Lin, Tingting; Islam, Omedul; Heese, Klaus

doi:10.1038/sj.cr.7310107

Cited by 85 publications

(62 citation statements)

References 178 publications

(285 reference statements)

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“…Note: due to the overlapping clusters of cells present in the SVZ (see Figure 2) and the difficulty in unambiguously identifying single cells, cell numbers were not quantified for this region. [15]. Furthermore, ABCA2 is a marker of neural progenitors in the adult mouse brain [16].…”

Section: Resultsmentioning

confidence: 99%

“…Our rationale for asking this question is based on previous studies showing that other ABC transporters including two that, like ABCA7, are also from the "Class-A" subfamily (i.e. ABCA2, ABCA3) appear to play a role in neurogenesis [15,16]. These studies along with the extensive literature highlighting the importance of adult neurogenesis in AD (see [17][18][19][20], for recent comprehensive reviews of this area) prompted our present study in which we have assessed cellular proliferation and neurogenesis in the dentate gyrus (DG) and subventricular zone (SVZ) of both WT and Abca7 − / − adult male mice.…”

Section: Introductionmentioning

confidence: 99%

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Abca7 deletion does not affect adult neurogenesis in the mouse

Karl

Garner

2016

Bioscience Reports

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SynopsisATP-binding cassette transporter A7 (ABCA7) is highly expressed in the brain. Recent genome-wide association studies (GWAS) have identified ABCA7 single nucleotide polymorphisms (SNPs) that increase Alzheimer's disease (AD) risk, however, the mechanisms by which ABCA7 may control AD risk remain to be fully elucidated. Based on previous research suggesting that certain ABC transporters may play a role in the regulation of neurogenesis, we conducted a study of cell proliferation and neurogenic potential using cellular bromodeoxyuridine (BrdU) incorporation and doublecortin (DCX) immunostaining in adult Abca7 deficient mice and wild-type-like (WT) littermates. In the present study counting of BrdU-positive and DCX-positive cells in an established adult neurogenesis site in the dentate gyrus (DG) indicated there were no significant differences when WT and Abca7 deficient mice were compared. We also measured the area occupied by immunohistochemical staining for BrdU and DCX in the DG and the subventricular zone (SVZ) of the same mice and this confirmed that ABCA7 does not play a significant role in the regulation of cell proliferation or neurogenesis in the adult mouse.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Abca7 deletion does not affect adult neurogenesis in the mouse

Karl

Garner

2016

Bioscience Reports

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“…Correspondingly, the NS and GL261 cells, besides showing the highest Mrp1 expression levels, were also the population with more BrdU+ cells, suggesting that multidrug transporters not only give resistance, but also allow and are needed for NSPC [66] and tumor cell [67] proliferation. Autophagy has been implicated in normal brain development [68,69] , and an abnormality in the autophagic process is related to malignant diseases.…”

Section: Protein Signatures With Gl261mentioning

confidence: 99%

Early Differentiating Mouse Astroglial Progenitors Share Common Protein Signatures with GL261 Glioma Cells

Brites¹

2016

JSRB

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Neural stem cells (NSC) biology is being applied to tumor research because these cells have been shown to share key properties with cancer cells. Gliomas represent the most common brain tumor, and neural stem/progenitor cells (NSPC) or early-differentiated cell type lineages may be on its origin. Here, we identified the developmental stage of the differentiation process of NSC into astrocytes that showed the highest number of tumorigenic similarities.NSPC were grown as neurospheres and astroglial differentiation was induced during 7 days in vitro (DIV). Cellular characterization was evaluated by specific neural markers at two developmental windows, i.e. NSPC/astrocyte progenitors from neurospheres until 3 DIV, and differentiating astrocytes thereafter. Predominance of immature Sox2-positive cells was verified in the first window and a prevalence of GFAP-positive cells in the second one. We then compared some tumor-related markers in GL261 glioma cells with such differentiating periods. The early progenitor cells (until 2 DIV) were those with the closest resemblances to the glioma cell line regarding BrdU incorporation, expression of microtubule-associated protein light chain 3 and of angiogenic factors (VEGF/VEGFR2), as well as S100B release. Our results suggest that early differentiating astroglial progenitors may be more susceptible to malignant transformation.

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“…ABC-transporters, particularly P-gp and BCRP, are transiently expressed at high levels in human neural stem/progenitor cells (hNSPCs) but are downregulated in differentiated hNSPCs, and in normal conditions, they are expressed in a highly regulated manner, with the highest expression in primitive cells and subsequent down-regulation following commitment to differentiation [74]. Furthermore, the constitutive deficiency of ABC-transporters leads to distinct impairments in neural stem/progenitor cells maturation and adult neurogenesis in vivo, indicating a functional role of ABC-transporters in stem cell maintenance and differentiation [75].…”

Section: P-gp As Stem Cell Marker Of Brain Malformation/tumor Refractmentioning

confidence: 99%