ABC transporter Pdr5 is required for cantharidin resistance in Saccharomyces cerevisiae

Swagatika, Swati; Tomar, Raghuvir Singh

doi:10.1016/j.bbrc.2021.03.074

Cited by 5 publications

(5 citation statements)

References 33 publications

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“…Cantharidin is a terpenoid that is produced by blister beetles and targets the remodeling processes of GPI‐proteins by affecting Cdc1 activity in the endoplasmic reticulum and impairs their sorting, which causes aggregation of GPI‐anchored proteins [79]. In yeast cells, the cantharidin‐resistant gene CRG1 and ABC transporter Pdr5 are required for cantharidin tolerance [80]. Therefore, we first checked the growth of flocculating cells upon cantharidin exposure.…”

Section: Resultsmentioning

confidence: 99%

Dissecting the role of mitogen‐activated protein kinase Hog1 in yeast flocculation

Kumawat,

Tomar

2024

The FEBS Journal

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Living organisms are frequently exposed to multiple biotic and abiotic stress forms during their lifetime. Organisms cope with stress conditions by regulating their gene expression programs. In response to different environmental stress conditions, yeast cells activate different tolerance mechanisms, many of which share common signaling pathways. Flocculation is one of the key mechanisms underlying yeast survival under unfavorable environmental conditions, and the Tup1‐Cyc8 corepressor complex is a major regulator of this process. Additionally, yeast cells can utilize different mitogen‐activated protein kinase (MAPK) pathways to modulate gene expression during stress conditions. Here, we show that the high osmolarity glycerol (HOG) MAPK pathway is involved in the regulation of yeast flocculation. We observed that the HOG MAPK pathway was constitutively activated in flocculating cells, and found that the interaction between phosphorylated Hog1 and the FLO genes promoter region increased significantly upon sodium chloride exposure. We found that treatment of cells with cantharidin decreased Hog1 phosphorylation, causing a sharp reduction in the expression of FLO genes and the flocculation phenotype. Similarly, deletion of HOG1 in yeast cells reduced flocculation. Altogether, our results suggest a role for HOG MAPK signaling in the regulation of FLO genes and yeast flocculation.

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Section: Resultsmentioning

confidence: 99%

Dissecting the role of mitogen‐activated protein kinase Hog1 in yeast flocculation

Kumawat,

Tomar

2024

The FEBS Journal

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“…The antimicrobial activity of the polyamines was determined using a Gram-negative bacteria (Escherichia coli JM101), a Gram-positive bacteria (Staphylococcus aureus BH1), and two strains of the yeast Saccharomyces cerevisiae (laboratory strain BY4741 carrying the YEplac195 plasmid or its derived one YEplac195-PDR5, named PDR5 from now on). This plasmid allows for the overexpression of the gene encoding Pdr5, an ATP-binding cassette (ABC) transporter, which can export toxic substances out of the cell (see [36] and references therein). For comparison purposes, commercial antibacterial (doxycycline, gentamicin, ciprofloxacin, and ampicillin) and antifungal (fluconazole, cycloheximide, and amphotericin B) agents were also included as control in our analyses.…”

Section: Determination Of the Antimicrobial Activitymentioning

confidence: 99%

Antimicrobial Properties of New Polyamines Conjugated with Oxygen-Containing Aromatic Functional Groups

Inclán,

Torres Hernández,

Martínez Serra

et al. 2023

Molecules

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Antibiotic resistance is now a first-order health problem, which makes the development of new families of antimicrobials imperative. These compounds should ideally be inexpensive, readily available, highly active, and non-toxic. Here, we present the results of our investigation regarding the antimicrobial activity of a series of natural and synthetic polyamines with different architectures (linear, tripodal, and macrocyclic) and their derivatives with the oxygen-containing aromatic functional groups 1,3-benzodioxol, ortho/para phenol, or 2,3-dihydrobenzofuran. The new compounds were prepared through an inexpensive process, and their activity was tested against selected strains of yeast, as well as Gram-positive and Gram-negative bacteria. In all cases, the conjugated derivatives showed antimicrobial activity higher than the unsubstituted polyamines. Several factors, such as the overall charge at physiological pH, lipophilicity, and the topology of the polyamine scaffold were relevant to their activity. The nature of the lipophilic moiety was also a determinant of human cell toxicity. The lead compounds were found to be bactericidal and fungistatic, and they were synergic with the commercial antifungals fluconazole, cycloheximide, and amphotericin B against the yeast strains tested.

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“…Cantharidin is a terpenoid that is produced by blister beetles and targets the remodeling processes of GPI-proteins by affecting the Cdc1 activity in ER and impair their sorting which causes aggregation of GPI-anchored proteins [74]. In yeast cells, the cantharidin-resistant gene CRG1 and ABC transporter Pdr5 are required for cantharidin tolerance [75]. Therefore, we rst checked the growth of occulating cells upon cantharidin exposure.…”

Section: Flocculating Cells Show Hypersensitivity To Gpi-anchored Pro...mentioning

confidence: 99%

Dissecting the role of HOG mitogen-activated protein kinase signaling in the regulation of Yeast Flocculation

Kumawat

Tomar

2023

Preprint

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The living organisms are frequently exposed to multiple biotic and abiotic stress forms during their lifetime. Organisms cope with stress conditions by regulating their gene expression programs. In response to different environmental stress conditions, yeast cells activate different tolerance mechanisms. Many of these mechanisms share common signaling pathways. Flocculation of yeast is one of the key mechanisms to survive under unfavorable environmental conditions. The Tup1-Cyc8 corepressor complex is a major regulator of yeast flocculation. In addition, yeast cells also utilize different MAPK pathways to regulate the expression of genes during stress conditions. Here we present the pieces of evidence that the HOG MAPK pathway is involved in the regulation of yeast flocculation phenotype. We observed constitutive activation of the HOG MAPK pathway in flocculating cells. The binding of phosphorylated Hog1 at the FLO genes promoter increases significantly upon sodium chloride exposure. We found that the treatment of cells with cantharidin reduces the Hog1 phosphorylation, causing a sharp reduction in the expression of FLO genes and flocculation phenotype. Similarly, deletion of the HOG1 in flocculating cells, resulted in the reduction of flocculation. Altogether our results suggest the involvement of HOG MAPK in the regulation of FLO genes and flocculation of yeast.

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ABC transporter Pdr5 is required for cantharidin resistance in Saccharomyces cerevisiae

Cited by 5 publications

References 33 publications

Dissecting the role of mitogen‐activated protein kinase Hog1 in yeast flocculation

Dissecting the role of mitogen‐activated protein kinase Hog1 in yeast flocculation

Antimicrobial Properties of New Polyamines Conjugated with Oxygen-Containing Aromatic Functional Groups

Dissecting the role of HOG mitogen-activated protein kinase signaling in the regulation of Yeast Flocculation

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