Abalone Haliotis discus hannai Intestine Digests with Different Molecule Weights Inhibit MMP-2 and MMP-9 Expression in Human Fibrosarcoma Cells

Abstract

The abalone Haliotis discus hannai, is one of the economically important species in the fisheries industry. Abalone intestines are one of the by-products of its processing. To investigate its bioactive potential, abalone intestine was digested using an in vitro gastrointestinal (GI) digestion system containing pepsin, trypsin, and α-chymotrypsin. The abalone intestine G1 digests (AIGIDs) produced by the GI digestion system were fractionated into AIGID I (> 100 kDa), AIGID II (10-100 kDa), and AIGID III (1-10 kDa) using an ultrafiltration membrane system. Of the three digests, AIGID II and AIGID III exhibited inhibitory effects against matrix metalloproteinase-2 and -9 (MMP-2, MMP-9) in HT1080 human fibrosarcoma cells. Both fractions potently inhibited gelatine digestion by MMP-2 and MMP-9 treated with phorbol 12-myristate 13-acetate (PMA) and migration of HT1080 cells in dose dependently. Furthermore, AIGID II and III attenuated expression of p65, a component of nuclear transcription factor kappa B. These results indicate that of the abalone intestine digests inhibit MMP-2 and MMP-9. Thus, the AIGIDs or their active components may have preventive and therapeutic potential for diseases associated with MMP-2 and MMP-9 activation in fibrosarcoma cells.

Inhibitory effect of the carnosine-gallic acid synthetic peptide on MMP-2 and MMP-9 in human fibrosarcoma HT1080 cells

“…TIMPs are secretive glycoproteins that inhibit MMP activity, thereby attenuating ECM degradation , and uPA and uPAR facilitate tumor cell invasion . Development of non‐toxic natural MMP inhibitors from marine resources is of great interest among researchers, and several MMP inhibitors have been identified from marine sources such as abalone , chitosan, chitin , and glucosamine . Thus, the RT–PCR analysis revealed that MMP‐2, MMP‐9, uPA, and uPAR mRNA levels following treatment with CGP were lower than those of carnosine.…”

Indole‑6‑carboxaldehyde isolated from Sargassum thunbergii inhibits the expression and secretion of matrix metalloproteinase‑9

“…The MAPK and NF-κ B pathways are generally recognized for their role in the regulation of various physiological processes, including cell proliferation, apoptosis and invasion (3,7,52). According to previous studies, MAPK and NF-κB pathways are known to promote MMP-9 expression in PMA-stimulated HT1080 cells (42,53).…”

“…The matrix metalloproteinases (MMPs) are important members of the zinc-dependent endopeptidase family and have been classified into eight subgroups, which include collagenases, matrilysin, metalloelastase, gelatinases, enamelysin and stromelysins (2). These enzymes target several components of the ECM, such as collagen, elastin and fibronectin; thus, they serve a crucial role in its degradation (3). In addition, MMPs are involved in a variety of physiological and pathological processes, including angiogenesis, tissue remodeling, and tumor invasion and metastasis (4)(5)(6).…”

A Novel Peptide from Abalone (Haliotis discus hannai) to Suppress Metastasis and Vasculogenic Mimicry of Tumor Cells and Enhance Anti-Tumor Effect In Vitro

“…More and more studies have found that abalone consists of many vital moieties like good protein, fatty acid and polysaccharides, which not only provides the basic nutrition for human health, but possesses the ability for anti-microbial, anti-oxidant, anti-thrombotic, anti-inflammatory and anti-cancer [14,15,16,17,18] uses. Qian [19] found that two peptides from abalone can suppress MMP-2 and MMP-9 expressions to inhibit the migration of HT1080 cells. A novel antimicrobial peptide, Ranatuerin-2PLx, showed inhibitory potential in the proliferation of cancer cells [2].…”