ABA Represses the Expression of Cell Cycle Genes and May Modulate the Development of Endodormancy in Grapevine Buds

Vergara, Ricardo; Noriega, Ximena; Aravena, Karla; Prieto, Humberto; Pérez, Francisco J.

doi:10.3389/fpls.2017.00812

Cited by 59 publications

(33 citation statements)

References 42 publications

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“…In this model, a single intravitreal injection of ABA significantly inhibited aberrant growth of blood vessels without inducing retinal stress, as demonstrated by unaltered GFAP expression. These effects are consistent with the function of ABA in seed dormancy during which a negative correlation between the tissue content of ABA and transcript levels of cell cycle and growth factor genes was found (Vergara et al, 2017). Given that cells are more sensitive to death stimuli when they are actively cycling, the effect of ABA culminates in desensitization of cells to mitogenic stimuli.…”

Section: Discussionsupporting

confidence: 80%

Abscisic acid – an anti-angiogenic phytohormone that modulates the phenotypical plasticity of endothelial cells and macrophages

Chaqour

Lee

Ravichandra

et al. 2018

Journal of Cell Science

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Abscisic acid (ABA) has shown anti-inflammatory and immunoregulatory properties in preclinical models of diabetes and inflammation. Herein, we studied the effects of ABA on angiogenesis, a strictly controlled process that, when dysregulated, leads to severe angiogenic disorders including vascular overgrowth, exudation, cellular inflammation and organ dysfunction. By using a 3D sprouting assay, we show that ABA effectively inhibits migration, growth and expansion of endothelial tubes without affecting cell viability. Analyses of the retinal vasculature in developing normoxic and hyperoxic mice challenged by oxygen toxicity reveal that exogenously administered ABA stunts the development and regeneration of blood vessels. In these models, ABA downregulates endothelial cell (EC)-specific growth and migratory genes, interferes with tip and stalk cell specification, and hinders the function of filopodial protrusions required for precise guidance of vascular sprouts. In addition, ABA skews macrophage polarization towards the M1 phenotype characterized by anti-angiogenic marker expression. In accordance with this, ABA treatment accelerates macrophage-induced programmed regression of fetal blood vessels. These findings reveal protective functions of ABA against neovascular growth through modulation of EC and macrophage plasticity, suggesting the potential utility of ABA as a treatment in vasoproliferative diseases.

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Section: Discussionsupporting

confidence: 80%

Abscisic acid – an anti-angiogenic phytohormone that modulates the phenotypical plasticity of endothelial cells and macrophages

Chaqour

Lee

Ravichandra

et al. 2018

Journal of Cell Science

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“…These results provide further support for the proposition that endogenous ABA inhibits grapevine bud dormancy release and that its degradation removes such repression and enhances bud break. In agreement, it was recently shown that ABA inhibits the expression of several cell cycle genes within latent grapevine buds (Vergara, Noriega, Aravena, Prieto, & Pérez, ).…”

Section: Discussionsupporting

confidence: 65%

“…In agreement, regulation of bud activity via modulation of ABA metabolism and/or signalling gained recent significant support that collectively suggests ABA‐mediated repression of growth resumption of both apical and lateral buds during para and endodormancy of annuals and woody perennials. Cell cycle arrest, plasmodesmata closure, regulation of the activities of callose synthase and glucanases, and regulation of movement/availability of FT to the bud meristem are among the proposed targets regulated by ABA, directly or indirectly (Arend et al, ; Tarancón et al, ; Tylewicz et al, ; Vergara et al, ; Yao & Finlayson, ). In light of the central role of ABA in regulation of seed germination, the assumption that conserved primary regulatory mechanism exists that control activity of the meristems may be supported.…”

Section: Resultsmentioning

confidence: 99%

Abscisic acid catabolism enhances dormancy release of grapevine buds

Zheng

Acheampong

Shi

et al. 2018

Plant Cell & Environment

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The molecular mechanism regulating dormancy release in grapevine buds is as yet unclear. It was formerly proposed that dormancy is maintained by abscisic acid (ABA)-mediated repression of bud-meristem activity and that removal of this repression triggers dormancy release. It was also proposed that such removal of repression may be achieved via natural or artificial up-regulation of VvA8H-CYP707A4, which encodes ABA 8'-hydroxylase, and is the most highly expressed paralog in grapevine buds. The current study further examines these assumptions, and its experiments reveal that (a) hypoxia and ethylene, stimuli of bud dormancy release, enhance expression of VvA8H-CYP707A4 within grape buds, (b) the VvA8H-CYP707A4 protein accumulates during the natural transition to the dormancy release stage, and (c) transgenic vines overexpressing VvA8H-CYP707A4 exhibit increased ABA catabolism and significant enhancement of bud break in controlled and natural environments and longer basal summer laterals. The results suggest that VvA8H-CYP707A4 functions as an ABA degrading enzyme, and are consistent with a model in which the VvA8H-CYP707A4 level in the bud is up-regulated by natural and artificial bud break stimuli, which leads to increased ABA degradation capacity, removal of endogenous ABA-mediated repression, and enhanced regrowth. Interestingly, it also hints at sharing of regulatory steps between latent and lateral bud outgrowth.

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“…ABA suppresses cell cycle progression through suppression of CYC and CDK genes and activation of CDK inhibitors (Meguro and Sato, ; Vergara et al ., ). In addition to the known cell cycle genes, we identified that EL2 , a plant‐specific cell cycle inhibitor of cell cycle progression, and its close paralogue, EL2like , are involved in SL‐mediated axillary bud dormancy.…”

Section: Discussionmentioning

confidence: 97%

Developmental analysis of the early steps in strigolactone‐mediated axillary bud dormancy in rice

et al. 2019

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SummaryBy contrast with rapid progress in understanding the mechanisms of biosynthesis and signaling of strigolactone (SL), mechanisms by which SL inhibits axillary bud outgrowth are less well understood. We established a rice (Oryza sativa L.) hydroponic culture system to observe axillary buds at the critical point when the buds enter the dormant state. In situ hybridization analysis indicated that cell division stops in the leaf primordia of the buds entering dormancy. We compared transcriptomes in the axillary buds isolated by laser capture microdissection before and after entering the dormant state and identified genes that are specifically upregulated or downregulated in dormant buds respectively, in SL‐mediated axillary bud dormancy. Typically, cell cycle genes and ribosomal genes are included among the active genes while abscisic acid (ABA)‐inducible genes are among the dormant genes. Application of ABA to the hydroponic culture suppressed the growth of axillary buds of SL mutants to the same level as wild‐type (WT) buds. Tiller number was decreased in the transgenic lines overexpressing OsNCED1, the gene that encodes ABA biosynthesis enzyme. These results indicated that the main site of SL function is the leaf primordia in the axillary bud and that ABA is involved in SL‐mediated axillary bud dormancy.

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ABA Represses the Expression of Cell Cycle Genes and May Modulate the Development of Endodormancy in Grapevine Buds

Cited by 59 publications

References 42 publications

Abscisic acid – an anti-angiogenic phytohormone that modulates the phenotypical plasticity of endothelial cells and macrophages

Abscisic acid – an anti-angiogenic phytohormone that modulates the phenotypical plasticity of endothelial cells and macrophages

Abscisic acid catabolism enhances dormancy release of grapevine buds

Developmental analysis of the early steps in strigolactone‐mediated axillary bud dormancy in rice

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