ABA-Regulated G Protein Signaling in <i>Arabidopsis</i> Guard Cells: A Proteomic Perspective

Zhao, Zhixin; Stanley, Bruce A.; Zhang, Wei; Assmann, Sarah M.

doi:10.1021/pr901011h

Cited by 77 publications

(78 citation statements)

References 71 publications

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“…Differentially expressed proteins were identified as those with normalized total intensity ratios (40 1C:46 1C) 41.2 or o0.8 combined with a Rank Product P-value p0.05 (except where noted), similar to methods used in other studies (Williamson et al, 2008;Dobbin et al, 2010;Soares et al, 2010;Zhao et al, 2010;Han et al, 2011;Jain et al, 2011;Muthukrishnan et al, 2011). Rank Product, commonly used in microarray experiments, is a non-parametric statistical method based on ranks of fold changes (Breitling et al, 2004).…”

Section: Proteome-based Functional Analysesmentioning

confidence: 99%

Elevated temperature alters proteomic responses of individual organisms within a biofilm community

et al. 2014

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Microbial communities that underpin global biogeochemical cycles will likely be influenced by elevated temperature associated with environmental change. Here, we test an approach to measure how elevated temperature impacts the physiology of individual microbial groups in a community context, using a model microbial-based ecosystem. The study is the first application of tandem mass tag (TMT)-based proteomics to a microbial community. We accurately, precisely and reproducibly quantified thousands of proteins in biofilms growing at 40, 43 and 46 1C. Elevated temperature led to upregulation of proteins involved in amino-acid metabolism at the level of individual organisms and the entire community. Proteins from related organisms differed in their relative abundance and functional responses to temperature. Elevated temperature repressed carbon fixation proteins from two Leptospirillum genotypes, whereas carbon fixation proteins were significantly upregulated at higher temperature by a third member of this genus. Leptospirillum group III bacteria may have been subject to viral stress at elevated temperature, which could lead to greater carbon turnover in the microbial food web through the release of viral lysate. Overall, these findings highlight the utility of proteomics-enabled community-based physiology studies, and provide a methodological framework for possible extension to additional mixed culture and environmental sample analyses.

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Section: Proteome-based Functional Analysesmentioning

confidence: 99%

Elevated temperature alters proteomic responses of individual organisms within a biofilm community

et al. 2014

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“…Production of reactive oxygen species (ROS) in response to the air pollutant ozone is also impaired in a mutant lacking the Ga subunit (Joo et al, 2005). The heterotrimeric G proteins also participate in ROS metabolism in plant cells (Wei et al, 2008;Zhao et al, 2010). During stomatal movement, G proteins mediate H 2 O 2 production induced by ABA (Zhang et al, 2011), ExtCaM (Chen et al, 2004;Li et al, 2009), and extracellular ATP (Hao et al, 2012) as well as NO production induced by ExtCaM in guard cells ).…”

mentioning

confidence: 99%

Role and Interrelationship of Gα Protein, Hydrogen Peroxide, and Nitric Oxide in Ultraviolet B-Induced Stomatal Closure in Arabidopsis Leaves

Zhang

et al. 2013

Plant Physiology

123

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Heterotrimeric G proteins have been shown to transmit ultraviolet B (UV-B) signals in mammalian cells, but whether they also transmit UV-B signals in plant cells is not clear. In this paper, we report that 0.5 W m 22 UV-B induces stomatal closure in Arabidopsis (Arabidopsis thaliana) by eliciting a cascade of intracellular signaling events including Ga protein, hydrogen peroxide (H 2 O 2 ), and nitric oxide (NO). UV-B triggered a significant increase in H 2 O 2 or NO levels associated with stomatal closure in the wild type, but these effects were abolished in the single and double mutants of AtrbohD and AtrbohF or in the Nia1 mutants, respectively. Furthermore, we found that UV-B-mediated H 2 O 2 and NO generation are regulated by GPA1, the Ga-subunit of heterotrimeric G proteins. UV-B-dependent H 2 O 2 and NO accumulation were nullified in gpa1 knockout mutants but enhanced by overexpression of a constitutively active form of GPA1 (cGa). In addition, exogenously applied H 2 O 2 or NO rescued the defect in UV-B-mediated stomatal closure in gpa1 mutants, whereas cGa AtrbohD/AtrbohF and cGa nia1 constructs exhibited a similar response to AtrbohD/ AtrbohF and Nia1, respectively. Finally, we demonstrated that Ga activation of NO production depends on H 2 O 2 . The mutants of AtrbohD and AtrbohF had impaired NO generation in response to UV-B, but UV-B-induced H 2 O 2 accumulation was not impaired in Nia1. Moreover, exogenously applied NO rescued the defect in UV-B-mediated stomatal closure in the mutants of AtrbohD and AtrbohF. These findings establish a signaling pathway leading to UV-B-induced stomatal closure that involves GPA1-dependent activation of H 2 O 2 production and subsequent Nia1-dependent NO accumulation.

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“…However, a recent study has proposed the flowering time control protein A (FCA) and the chloroplastic magnesium protoporphyrin-IX chelatase H subunit (CHLH) as candidates for ABA receptors, both of which have been shown to bind ABA in vitro [196]. Two genes have been found to play a crucial role in the prevention of stomata opening -GPA1 (G PROTEIN ALPHA SUBUNIT 1) and PLDα1 (PHOSPHOLIPASE D AL-PHA 1) [197]. PLD-produced phosphatidic acid has been also shown to play an important role in the plant response to drought stress [198].…”

Section: + Rosmentioning

confidence: 99%

Programmed Cell Death as a Response to High Light, UV and Drought Stress in Plants

Wituszyńska¹,

Karpiński²

2013

Abiotic Stress - Plant Responses and Applications in Agriculture

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ABA-Regulated G Protein Signaling in Arabidopsis Guard Cells: A Proteomic Perspective

Cited by 77 publications

References 71 publications

Elevated temperature alters proteomic responses of individual organisms within a biofilm community

Elevated temperature alters proteomic responses of individual organisms within a biofilm community

Role and Interrelationship of Gα Protein, Hydrogen Peroxide, and Nitric Oxide in Ultraviolet B-Induced Stomatal Closure in Arabidopsis Leaves

Programmed Cell Death as a Response to High Light, UV and Drought Stress in Plants

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