ABA promotes starch synthesis and storage metabolism in dormant grapevine buds

Rubio, Sebastián Figueroa; Noriega, Ximena; Pérez, Francisco J.

doi:10.1016/j.jplph.2019.01.004

Cited by 39 publications

(36 citation statements)

References 40 publications

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“…Studies have shown that ABA inhibits AA activity during dormancy [32]. Rubio et al [33] suggested that ABA content was positively correlated with starch content during dormancy in grape buds. In our study, ABA-induced the expression of VvSS1 and VvSS3 and inhibited the expression of VvSUSs , VvSPS , and VvINVs .…”

Section: Discussionmentioning

confidence: 99%

Hydrogen cyanamide induces grape bud endodormancy release through carbohydrate metabolism and plant hormone signaling

et al. 2019

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BackgroundGrape buds exhibit non-uniform, or delayed, break in early spring in subtropical regions because the accumulation of chilling is insufficient. Hydrogen cyanamide (H2CN2, HC) can partially replace chilling to effectively promote bud sprouting and is used widely in warm winter areas. However, the exact underlying mechanism of grape bud release from endodormancy induced by HC remains elusive.ResultsIn this study, the transcriptome of grape winter buds under in vitro conditions following HC and water treatment (control) was analyzed using RNA-seq technology. A total of 6772 differentially expressed genes (DEGs) were identified. Furthermore, the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that starch and sucrose metabolism and plant hormone signaling transduction were most enriched out of the 50 total pathways. HC treatment induced the upregulated expression of sucrose synthase (SUS), sucrose phosphate synthase (SPS), α-amylase (AM), and β-amylase (BM) and downregulated expression of sucrose invertase (INV), hexokinase (HK), fructokinase (FK), soluble starch synthase (SS), and granule-bound starch synthase (GBSS). Hence, the starch concentration in the HC-treated group was significantly lower than that in control, whereas soluble sugar content in the HC-treated group increased quickly and was higher than that in control between 0 and 8 d. The concentration of indoleacetic acid (IAA) and zeatin (ZT) increased, whereas that of abscisic acid (ABA) and gibberellin (GA) decreased in HC treated group, which coincided with the expression level of genes involved in above hormone signals. The content of hydrogen peroxide (H2O2) and enzyme activity of superoxide dismutase (SOD) and peroxidase (POD) were increased in grape buds with HC treatment, whereas catalase (CAT) activity was decreased. HC treatment increased the expression of POD, SOD, primary amine oxidase (PAO), polyamine oxidase (PAOX), and glutathione peroxidase (GSH-Px).ConclusionBased on these results, it is possible to propose a mechanistic model that underlies the regulation of endodormancy release in grapevine buds by exogenous HC application.

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Section: Discussionmentioning

confidence: 99%

Hydrogen cyanamide induces grape bud endodormancy release through carbohydrate metabolism and plant hormone signaling

et al. 2019

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“…The antagonism between ABA and GA/CK, which is considered to be the key factor of dormancy, has also been revealed in plants with typical reproductive organs such as Solanum tuberosum L. [8], Zantedeschia [9] and Gladiolus hybridus [10]. ABA controls the dormancy and germination processes by regulating the expression of starch and sucrose metabolism genes such as AtAMY3, AtBAM1, VvSSs1, VvSSs3 and VvINV in Arabidopsis thaliana [11] and grape [12]. In Arabidopsis thaliana, AtGASA6, a gene involved in gibberellin biosynthesis, is a key factor involved in gibberellin, abscisic acid and carbohydrate metabolic pathways and interacts with RGL2 and AtEXPA1 to regulate seed germination [13].…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Transcriptome and targeted metabolome reveal the regulation network of Lilium davidii var. unicolor during dormancy release

Fan

Yang

et al. 2020

Preprint

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Background：Regulation and control of bulb dormancy are critical for ensuring annual production and high-level cultivation of lily. Application of low temperatures is the most effective method for breaking lily bulb dormancy, but the molecular regulatory mechanism underlying this response is unclear. Results：Herein, targeted metabolome and transcriptome analyses were performed with buds of Lilium davidii var. unicolor bulbs stored for 0, 50 and 100 days at 4°C. Dormancy release mainly depended on the accumulation of GA4 and GA7, which are synthesized by the "non13-hydroxylation" pathway, rather than GA3, and ABA was degraded in the process. The contents of nonbioactive GA9, GA15 and GA24, the precursors of GA4 synthesis, increased with bulb dormancy release. Altogether, 113,252 unique transcripts were de novo assembled through high-throughput transcriptome sequences, and 639 genes were continuously differentially expressed. Enrichment analysis showed that the carbohydrate metabolism and hormone metabolism pathways play major roles in dormancy release. Energy sources during carbohydrate metabolism mainly depend on glycolysis and the PPP pathway rather than on the TCA cycle. Association analysis of the transcriptome and target metabolome showed that genes related to ABA, GA, starch and sucrose metabolism and some TF families, such as MYB, WRKY, NAC and TCP, involved in dormancy release were highly correlated with the target metabolome. Coexpression analysis further confirmed that ABI5, PYL8, PYL4 and PP2C, vital ABA signaling elements, regulated GA3ox and GA20ox in the GA4 biosynthesis pathway. The TFs WRKY32, WRKY71, MYB, DAM14, NAC8, ICE1, bHLH93 and TCP15 also participated in the ABA/GA4 regulatory network, and ICE1 may be the key factor linking temperature signals and hormone metabolism. Conclusions：These results will help to reveal the bulb dormancy molecular mechanism and to develop new strategies for high-quality bulb production.

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“…There was also reasonable agreement with the seasonal dynamics reported for cv. Thompson Seedless (33°34’S; Parada et al, 2016; Rubio et al, 2016; Rubio et al, 2019), cv. Chardonnay and cv.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, the depth of dormancy varies widely. Measured as BB 50 , Rubio et al (2019 and references therein) showed a range in peak dormancy for cv. Thompson Seedless of ca .…”

Section: Introductionmentioning

confidence: 93%

The seasonal dynamics of bud dormancy in grapevine suggest a regulated checkpoint prior to acclimation

Velappan

Considine

et al. 2021

Preprint

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Grapevine (Vitis vinifera L.) displays wide plasticity to climate and seasonality, ranging from strongly deciduous to evergreen. Understanding the physiology of decisions to grow or quiesce is critical for improved crop management, prediction, and the adaptability of production to alternative climate scenarios. The perenniating bud (N+2) is a major economic unit and focus of study. Here we investigated the physiology and transcriptome of cv. Merlot buds grown in a temperate maritime climate from summer to spring in two consecutive years. The changes in bud respiration, hydration and internal tissue oxygen data were consistent with the transcriptome data. ABA-responsive gene processes prevailed upon the transition to a deep metabolic and cellular quiescence in the bud during autumn. Light, together with hypoxia and redox signalling presided over the resumption of nuclear and cellular growth in the transition to spring. Comparisons with transcriptome data from bud burst studies revealed a number of regulatory candidates for the orderly resumption of growth in spring, including components that may integrate light and temperature signalling. Importantly however, the bud burst forcing data, which is widely used as a measure of bud dormancy, were not consistent with the physiological and transcription data. We hypothesise the existence of a physiological checkpoint following bud set in summer, which if not met results in extreme quiescence. Collectively this is the most integrated developmental dataset of the latent bud of cultivated grapevine, and establishes a platform for systems approaches to study seasonal plasticity.

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ABA promotes starch synthesis and storage metabolism in dormant grapevine buds

Cited by 39 publications

References 40 publications

Hydrogen cyanamide induces grape bud endodormancy release through carbohydrate metabolism and plant hormone signaling

Hydrogen cyanamide induces grape bud endodormancy release through carbohydrate metabolism and plant hormone signaling

WITHDRAWN: Transcriptome and targeted metabolome reveal the regulation network of Lilium davidii var. unicolor during dormancy release

The seasonal dynamics of bud dormancy in grapevine suggest a regulated checkpoint prior to acclimation

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