ABA Levels and Sensitivity in Developing Wheat Embryos of Sprouting Resistant and Susceptible Cultivars

Walker-Simmons, M. K.

doi:10.1104/pp.84.1.61

Cited by 459 publications

(340 citation statements)

References 22 publications

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“…3 and 4). The enhanced expression of a β-hydroxylase gene in the embryo tissue may provide the required intermediate for synthesis of abscisic acid (ABA), which increases towards maturation of wheat grains (Walker-Simmons, 1987). Further studies are required to understand the spatial expression of carotenogenic genes and homeologs in different sections of wheat grains and how they contribute to β-carotene/β-carotene-derived xanthophyll accumulation in these tissues.…”

Section: The Predominant Expression Of Hyd1 Particularly Hyd-b1 In mentioning

confidence: 99%

Cloning and comparative analysis of carotenoid β-hydroxylase genes provides new insights into carotenoid metabolism in tetraploid (Triticum turgidum ssp. durum) and hexaploid (Triticum aestivum) wheat grains

Qin

Zhang²,

Dubcovsky³

et al. 2012

Plant Mol Biol

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Carotenoid β-hydroxylases attach hydroxyl groups to the β-ionone rings (β-rings) of carotenoid substrates, resulting in modified structures and functions of carotenoid molecules. We cloned and characterized two genes (each with three homeologs), HYD1 and HYD2, which encode β-hydroxylases in wheat. The results from bioinformatic and nested degenerate PCR analyses collectively suggest that HYD1 and HYD2 may represent the entire complement of non-heme diiron β-hydroxylases in wheat. The homeologs of wheat HYDs exhibited major β-ring and minor ε-ring hydroxylation activities in carotenoid-accumulating E. coli strains. Distinct expression patterns were observed for different HYD genes and homeologs in vegetative tissues and developing grains of tetraploid and hexaploid wheat, suggesting their functional divergence and differential regulatory control in tissue-, grain development-, and ploidy-specific manners. An intriguing observation was that the expression of HYD1, particularly HYD-B1, reached highest levels at the last stage of tetraploid and hexaploid grain development, suggesting that carotenoids (at least xanthophylls) were still actively synthesized in mature grains. This result challenges the common perception that carotenoids are simply being turned over during wheat grain development after their initial biosynthesis at the early grain development stages. Overall, this improved understanding of carotenoid biosynthetic gene expression and carotenoid metabolism in wheat grains will contribute to the improvement of the nutritional value of wheat grains for human consumption.

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Section: The Predominant Expression Of Hyd1 Particularly Hyd-b1 In mentioning

confidence: 99%

Cloning and comparative analysis of carotenoid β-hydroxylase genes provides new insights into carotenoid metabolism in tetraploid (Triticum turgidum ssp. durum) and hexaploid (Triticum aestivum) wheat grains

Qin

Zhang²,

Dubcovsky³

et al. 2012

Plant Mol Biol

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“…The elutants were concentrated to dryness by vacuum-evaporation and re-suspended in tris-buffered saline before enzyme-linked immunosorbent assay (ELISA). ABA content was quantified by ELISA [28]. The ABA immunoassay detection kit was purchased from the Nanjing Agriculture University.…”

Section: Aba Determinationmentioning

confidence: 99%

Upland rice and lowland rice exhibited different PIP expression under water deficit and ABA treatment

et al. 2006

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Aquaporins play a significant role in plant water relations. To further understand the aquaporin function in plants under water stress, the expression of a subgroup of aquaporins, plasma membrane intrinsic proteins (PIPs), was studied at both the protein and mRNA level in upland rice (Oryza sativa L. cv. Zhonghan 3) and lowland rice (Oryza sativa L. cv. Xiushui 63) when they were water stressed by treatment with 20% polyethylene glycol (PEG). Plants responded differently to 20% PEG treatment. Leaf water content of upland rice leaves was reduced rapidly. PIP protein level increased markedly in roots of both types, but only in leaves of upland rice after 10 h of PEG treatment. At the mRNA level, OsPIP1;2, Os-PIP1;3, OsPIP2;1 and OsPIP2;5 in roots as well as OsPIP1;2 and OsPIP1;3 in leaves were significantly up-regulated in upland rice, whereas the corresponding genes remained unchanged or down-regulated in lowland rice. Meanwhile, we observed a significant increase in the endogenous abscisic acid (ABA) level in upland rice but not in lowland rice under water deficit. Treatment with 60 µM ABA enhanced the expression of OsPIP1;2, OsPIP2;5 and OsPIP2;6 in roots and OsPIP1;2, OsPIP2;4 and OsPIP2;6 in leaves of upland rice. The responsiveness of PIP genes to water stress and ABA were different, implying that the regulation of PIP genes involves both ABA-dependent and ABA-independent signaling pathways during water deficit.

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“…Xylem sap [ABA] was measured by an indirect ELISA (Walker-Simmons, 1987) using monoclonal antibody to ABA (Agdia, Elkhart, IN, USA) and (p) ABA as a standard, as described previously (Duan et al, 1996). Validation of the ELISA assay for use with unpurified xylem exudate was confirmed by dilution and spike recovery tests for each species for nonspecific interference (Walker-Simmons & Abrams, 1991).…”

Section: Plant Measurementsmentioning

confidence: 99%

Correlations of stomatal conductance with hydraulic and chemical factors in several deciduous tree species in a natural habitat

et al. 2000

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Recent research in whole-plant stomatal physiology, conducted largely with potted plants in controlled environments, suggests that stomatal conductance (g s ) might be more closely linked to plant chemical variables than to hydraulic variables. To test this in a field situation, seasonal g s was examined in relation to a number of plant and environmental variables in 11 temperate, deciduous forest tree species. Stomatal conductance was generally better correlated with environmental variables (air temperature, vapor pressure deficit, PPFD) than with plant variables, and slightly better correlated with plant hydraulic variables (shoot water and osmotic potentials) than with plant chemical variables (xylem sap ABA concentration, xylem sap pH). We examined a model, developed previously for maize, which describes regulation of g s by xylem sap ABA concentration with leaf water status acting to modify stomatal sensitivity to the ABA signal. This model explained slightly more variation in seasonal g s in the forest trees than did single plant variables but not more variation than most single environmental variables. Response surface models, especially those incorporating environmental variables, were more consistently successful at explaining g s across species.

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ABA Levels and Sensitivity in Developing Wheat Embryos of Sprouting Resistant and Susceptible Cultivars

Cited by 459 publications

References 22 publications

Cloning and comparative analysis of carotenoid β-hydroxylase genes provides new insights into carotenoid metabolism in tetraploid (Triticum turgidum ssp. durum) and hexaploid (Triticum aestivum) wheat grains

Cloning and comparative analysis of carotenoid β-hydroxylase genes provides new insights into carotenoid metabolism in tetraploid (Triticum turgidum ssp. durum) and hexaploid (Triticum aestivum) wheat grains

Upland rice and lowland rice exhibited different PIP expression under water deficit and ABA treatment

Correlations of stomatal conductance with hydraulic and chemical factors in several deciduous tree species in a natural habitat

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