ABA homeostasis and long-distance translocation are redundantly regulated by ABCG ABA importers

Drought is one of the major constraints to rain-fed agricultural production, especially under climate change conditions. Plants evolved an array of adaptive strategies that perceive stress stimuli and respond to these stress signals through specific mechanisms. Abscisic acid (ABA) is a premier signal for plants to respond to drought and plays a critical role in plant growth and development. ABA triggers a variety of physiological processes such as stomatal closure, root system modulation, organizing soil microbial communities, activation of transcriptional and post-transcriptional gene expression, and metabolic alterations. Thus, understanding the mechanisms of ABA-mediated drought responses in plants is critical for ensuring crop yield and global food security. In this review, we highlighted how plants adjust ABA perception, transcriptional levels of ABA- and drought-related genes, and regulation of metabolic pathways to alter drought stress responses at both cellular and the whole plant level. Understanding the synergetic role of drought and ABA will strengthen our knowledge to develop stress-resilient crops through integrated advanced biotechnology approaches. This review will elaborate on ABA-mediated drought responses at genetic, biochemical, and molecular levels in plants, which is critical for advancement in stress biology research.

show abstract

“…Stomatal conductance, and increased water use efficiency [125] AtPDR12/ABCG40 ABA uptake transporter [32] AIT1/NRT1.2…”

Section: Atabcg17-18mentioning

confidence: 99%

Mechanisms of Abscisic Acid-Mediated Drought Stress Responses in Plants

Aslam

Waseem

Jakada

et al. 2022

IJMS

134

View full text Add to dashboard Cite

show abstract

“…The half-size ABC transporters STR1 and STR2 are indispensable for mycorrhizal arbuscule formation in rice [ 64 ]. Increasing reports reveal that ABCG transporters perform important physiological functions in plant drought stress response by regulating stomatal closure, such as OsABCG5/RCN1 [ 50 ], AtABCG17 and AtABCG18 [ 67 ], AtABCG21 and AtABCG22 [ 39 , 68 ], AtABCG25 [ 69 ], and AtABCG40 [ 62 ], while OsABCG9 plays a critical role in the transportation of epicuticular wax and is essential for rice drought response [ 53 ]. Overexpression of the AtABCG19 transporter confers kanamycin resistance to transgenic plants, which may be related to zinc homeostasis in plants [ 51 , 52 ].…”

Section: Multiple Functions Of Abcg Transporters In Arabido...mentioning

confidence: 99%

ATP-Binding Cassette G Transporters and Their Multiple Roles Especially for Male Fertility in Arabidopsis, Rice and Maize

Shu

Fang

et al. 2022

IJMS

View full text Add to dashboard Cite

ATP-binding cassette subfamily G (ABCG) transporters are extensive in plants and play essential roles in various processes influencing plant fitness, but the research progress varies greatly among Arabidopsis, rice and maize. In this review, we present a consolidated nomenclature and characterization of the whole 51 ABCG transporters in maize, perform a phylogenetic analysis and classification of the ABCG subfamily members in maize, and summarize the latest research advances in ABCG transporters for these three plant species. ABCG transporters are involved in diverse processes in Arabidopsis and rice, such as anther and pollen development, vegetative and female organ development, abiotic and biotic stress response, and phytohormone transport, which provide useful clues for the functional investigation of ABCG transporters in maize. Finally, we discuss the current challenges and future perspectives for the identification and mechanism analysis of substrates for plant ABCG transporters. This review provides a basic framework for functional research and the potential application of ABCG transporters in multiple plants, including maize.

show abstract

“…In addition, translocation of ABA among cells and tissues are essential for activation of the ABA signaling pathway in systemic stress responses [15,16]. Due to the weak-acid nature of ABA and its declined diffusion which resulted from cytosolic alkalization under stressed conditions [17], the translocation of ABA mainly relies on active transporters from the ATP-binding cassette (ABCG) family [18][19][20][21][22], NRT1/PTR (NPF) [23], multidrug and toxic compound extrusion (MATE)-type/DTX family [24], and AWPM-19 family [25].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Organellar-Specific ABA Responses during Environmental Stresses in Tobacco Cells and Arabidopsis Plants

Wang

Zhou²,

Liang³

2022

Cells

View full text Add to dashboard Cite

Abscisic acid (ABA) is a critical phytohormone involved in multifaceted processes in plant metabolism and growth under both stressed and nonstressed conditions. Its accumulation in various tissues and cells has long been established as a biomarker for plant stress responses. To date, a comprehensive understanding of ABA distribution and dynamics at subcellular resolution in response to environmental cues is still lacking. Here, we modified the previously developed ABA sensor ABAleon2.1_Tao3 (Tao3) and targeted it to different organelles including the endoplasmic reticulum (ER), chloroplast/plastid, and nucleus through the addition of corresponding signal peptides. Together with the cytosolic Tao3, we show distinct ABA distribution patterns in different tobacco cells with the chloroplast showing a lower level of ABA in both cell types. In a tobacco mesophyll cell, organellar ABA displayed specific alterations depending on osmotic stimulus, with ABA levels being generally enhanced under a lower and higher concentration of salt and mannitol treatment, respectively. In Arabidopsis roots, cells from both the meristem and elongation zone accumulated ABA considerably in the cytoplasm upon mannitol treatment, while the plastid and nuclear ABA was generally reduced dependent upon specific cell types. In Arabidopsis leaf tissue, subcellular ABA seemed to be less responsive when stressed, with notable increases of ER ABA in epidermal cells and a reduction of nuclear ABA in guard cells. Together, our results present a detailed characterization of stimulus-dependent cell type-specific organellar ABA responses in tobacco and Arabidopsis plants, supporting a highly coordinated regulatory network for mediating subcellular ABA homeostasis during plant adaptation processes.

show abstract

ABA homeostasis and long-distance translocation are redundantly regulated by ABCG ABA importers

Abstract: ABCG17 and ABCG18 ABA importers redundantly regulate ABA homeostasis and long-distance translocation.

Cited by 42 publications

References 84 publications

Mechanisms of Abscisic Acid-Mediated Drought Stress Responses in Plants

Mechanisms of Abscisic Acid-Mediated Drought Stress Responses in Plants

ATP-Binding Cassette G Transporters and Their Multiple Roles Especially for Male Fertility in Arabidopsis, Rice and Maize

Characterization of Organellar-Specific ABA Responses during Environmental Stresses in Tobacco Cells and Arabidopsis Plants

Contact Info

Product

Resources

About