ABA alleviated soybean seedling stress exposed to UV-C radiation

Abstract

In order to understand the roles of abscisis acid in soybean exposed to UV-C radiation, soybean seedlings were exposed to UV-C radiation and ABA was applied. The chlorophyll content, active oxygen metabolism and flavonoids content of soybean seedlings were investigated. The present study revealed that UV-C radiation significantly attenuated the chlorophyll content and suppressed PSII activity. It was observed that the contents of H 2 O 2 , malondialdehyde and O 2 were markedly increased, and the activities of antioxidant enzymes such as superoxide dismutase, peroxidase and catalase were enhanced, so were the flavonoids content, whereas the seedling height and biomass were significantly reduced. ABA application improved the activities of superoxide dismutase, peroxidase, catalase and the contents of flavonoids, decreased the contents of H 2 O 2 , MDA and O 2-, and also increased the chlorophyll content and enhanced PSII performance, thus enhancing biomass accumulation. The results suggested that ABA alleviated UV-C damage to soybean seedlings by reducing ROS generation and enhancing antioxidase activity. The isoflavones daidzin and genistein were significantly responsive to UV-C.

Regulation of ROS Metabolism in Plants under Environmental Stress: A Review of Recent Experimental Evidence

“…In contrast, genotype C24 showed increased POD, APX, and GPX activities exposed to UV-B radiation (3.9 kJ m −2 , 4 d). UV-C radiation-induced enhancement of SOD and POD activities was also reported in G. max L. [ 130 ]. Seedlings of G. max L. grown under UV-C light with 0.284 mW cm −2 intensity (20 min d −1 ) resulted in enhanced activity of CAT, SOD, and POD that was further upregulated with exogenous application of AsA (10 mg L −1 ) [ 130 ].…”

“…Tripathi et al [ 129 ] observed increased contents of O 2 •− , H 2 O 2 , MDA, and higher EL in T. aestivum L. seedlings exposed to two levels of UV-B radiation: ambient (8.6 kJ m −2 d −1 ) and enhanced (ambient + 2.8 kJ m −2 d −1 ). G. max L. plants grown under UV-C light with 0.284 mW cm −2 intensity (20 min d −1 ) resulted in remarkably higher O 2 •− , H 2 O 2, and MDA contents [ 130 ]. Four hours of UV-B radiation causes higher levels of EL, MDA, and O 2 •− contents in Morus alba seedlings under dark conditions [ 131 ].…”