AB0120 Adiponectin could be a mediator of the presence of p gingivalis in patients with early rheumatoid arthritis

Abstract

BackgroundRheumatoid arthritis (RA) is a systemic chronic inflammatory disease characterised by joint destruction, deformity, lower functional status and decrease in life expectancy. The Porphyromonas gingivalis (P. gingivalis) has been reported to be significantly associated with RA. The adipokines are suggested to be a common link between periodontitis and RAObjectivesTo determine the association between serum adipokines levels and presence of P gingivalis in patients with early rheumatoid arthritis (eRA) compared to healthy individualsMethodsA cross sectional study was conducted. Patients with the diagnosis of (eRA) according to the ACR/EULAR 2010 criteria were studied in Bogota-Colombia. A complete medical history related to RA was obtained. Adiponectin levels measured by Luminex technology (MILLIPLEXMAP ), IL6 by chemiluminescence (Immulite 1000, Siemens) and leptin quantification by ELISA (Diasource, ), high-sensitivity CRP (hs-CRP) (Immulite 1000, Siemmens) and ESR (Test 1 THL Ali FAX), and the detection of periodontopathic bacteria was carried out by qPCR from subgingival plaque samples. Cancer, autoimmune disease, infection, antibiotic use, diabetes, orthodontics treatment were exclusion criteria. An association analysis was made to evaluate the relationship between adipokines levels and periodontal, rheumatologic conditions using X2 test, Mann Whitney test and logistic regression model was performed to confirm this associations. All the results were performed with a level of significance of 95%ResultsA total of 51 patients with eRA and 51 healthy individuals were matched by age and gender. The mean age in RA patients was 48,54±10,93 years, 80,39% were female, between RA patients, 17.64% were in high activity and 27.45% in remission by DAS 28-ESR. 45.1% had APCA>20 UE. 37.25% were overweight and 13.72% were obese. 82.35% of patients had treatment, of which 80.39% received treatment with conventional disease modifying therapy, being methotrexate the most frequent one in 86.8%. In turn, 64.7% and 51% had high leptin and adiponectin levels respectively. An association of high adiponectin levels was found with Body Mass Index-BMI <25 (p=0.017). The presence of P gingivalis in RA patients was 78.4% and in healthy individuals was 45,09% p=0001. The adiponectin levels>47755 pg/ml were found associated with the absence of P gingivalis (OR=0.078 95% CI 0.01–0.62) adjusted an ever smoking history of cigarette smoking, Body Mass Index>30 and high level of leptin and IL6.ConclusionsThe adiponectin plays an anti-inflammatory role in the pathophysiology of several chronic inflammatory diseases and it has been reported to inhibit LPS-induced NFκB nuclear translocation and to affect other LPS-triggered pathways. To promote the weight control in eRA could be to prevent the oral infectionReference[1] Le Sage F, et al. Mol Cell Endocrinol2017;446:102–110.Disclosure of InterestNone declared

High Levels of Leptin and Adipsin Are Associated with Clinical Activity in Early Rheumatoid Arthritis Patients with Overweight and Periodontal Infection

“…The levels of adipsin should also rise, being also produced by monocytes in response to infection, promoting the activation of complement, and favoring the adipsin-dependent pathway to recruit more neutrophils [ 31 , 32 ]. Previous analyses have shown a relationship between the high levels of adiponectin and the absence of P. gingivalis [ 33 ]. However, this relationship was not found in the current study.…”