Ab0003 interleukin 17a Rs2275913 Gene Polymorphism Is Related to Severity and Disease Activity of Rheumatoid Arthritis and Increased Serum Il17a Levels in Egyptian Patients.

Abstract

BackgroundInterleukin 17A (IL17A), one of the IL-17 family, performs a critical role in the inflamed synovial tissue of Rheumatoid Arthritis (RA) patients. Several studies have been performed to investigate the role of IL 17A rs2275913 polymorphism in RA pathogenesis, however, data about its role in RA severity and activity is inconsistent.ObjectivesWe aimed to investigate the influence of IL17A rs2275913 polymorphism on IL17A serum levels in Egyptian RA patients and disease severity and activity.MethodsThe study included 100 healthy subjects and 100 RA patients. Gene polymorphism rs2275913 was measured by Taqman genotyping assay in both patients and healthy control groups. IL17A serum levels and clinical and laboratory parameters were measured using the enzyme-linked immune sorbent assay (ELISA) technique.ResultsRA patients showed higher IL 17A serum levels than the control group (121.9 ± 24.3 ng/dl vs 20.4 ± 2.6 ng/dl, P < 0.001). RA patients showed a higher frequency of rs2275913 G allele than healthy subjects (P = 0.01). Patients carrying GG genotype showed higher values of all disease severity parameters including Rheumatoid Factor (52.79 ± 16.65 IU/ml, P<0.001) and Anti-Cyclic Citrullinated Peptide Antibody (36.6 ± 14.86 U/ml, P < 0.001). The high-risk GG genotype carriers had higher IL17A serum levels than the GA and AA genotypes carriers (129.74 ± 23.03 ng/dl vs 107.49 ± 19.85 ng/dl, P <0.001).ConclusionThe major allele of IL17A rs2275913 polymorphism was associated with higher IL17A serum levels, and greater RA severity. It is thus very likely that the rs2275913 polymorphism of IL17A gene is associated with an increased risk of RA, as well as with higher severity in Egyptian RA patients.References[1]Taams, L. S. (2020). Interleukin-17 in rheumatoid arthritis: Trials and tribulations.Journal of Experimental Medicine,217(3).[2]Shao, M., Xu, W., Yang, H., Chen, Y., Gao, X., Xu, S.,... & Pan, F. (2021). Interleukin-17 Gene Polymorphism (Rs2275913 G/A, Rs763780 C/T) in Rheumatoid arthritis： Meta-analysis Based on Ethnicity.Immunological Investigations,50(6), 685-699.Acknowledgements:NIL.Disclosure of InterestsNone Declared.