AB Initio Structural Analysis on Envelope (E) Glycoprotein of Dengue Virus (DENV) Type 1-4

Abstract

Any serotypes of dengue virus (DENV), which are DENV1-4, may lead to dengue infections.DENV spread via mosquito vector, primarily Aedes aegypti and Aedes albopictus mosquitoes.DENV is one of the most prevalent viruses, which is causing dengue fever (DF), dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS) that may be fatal. determined. An effective vaccine against DENV infections should be a tetravalent vaccine that can counter-attack all four serotypes. Therefore, E glycoproteins of all four serotypes was chosen to be analyzed. Conserved regions were found in 100 sequences of each DENV serotype.Multiple sequence alignments was done to obtain consensus sequence. Multiple bioinformatics tools were used for prediction of B-cell and T-cell epitopes based on antigenicity, hydrophilicity, beta-turn, flexibility and surface accessibility. Secondary and tertiary structures of E glycoprotein for each serotypes were predicted and aligned. Complete analysis showed that predicted epitopes were located within conserved regions, and DENV2 showed the highest percentage of mutation rates and had the highest similarity to DENV1, DENV3 and DENV4.