Ab initio conformational analysis of marinobufagenin molecule and molecular targets of the action of cardiotonic steroids

Rogachevskii, I. V.; Shelykh, T. N.; Подзорова, С. А.; Крылов, Б. В.; Plakhova, Vera B.

doi:10.1134/s1070428015110172

Cited by 1 publication

(1 citation statement)

References 14 publications

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“…Bufalin-3-suberoyl-l-arginine(E): 1 Table S1. Those data were in agreement with earlier published data for Bufalin-3-suberoyl-l-arginine [27,41].…”

Section: Ta B L E 2 Thesupporting

confidence: 93%

An efficient strategy based on liquid‐liquid extraction and pH‐zone‐refining counter‐current chromatography for selective enrichment, separation, and purification of alkaloids and organic Acids from natural products

et al. 2020

J of Separation Science

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This study presents an efficient strategy based on liquid‐liquid extraction and pH‐zone‐refining counter‐current chromatography for selective enrichment, separation, and purification of alkaloids and organic acids from natural products. First, an acid or base modified two‐phase solvent system with maximum or minimum partition coefficient was developed for the liquid‐liquid extraction of the crude extract. As a result, alkaloids or organic acids could be selectively enriched in the upper or lower phase. Then pH‐zone‐refining counter‐current chromatography was employed to separate and purify the selectively enriched alkaloids or organic acids efficiently. The selective enrichment and separation of five bufadienolide from toad venom of Bufo marinus was used as an example to show the advantage of this strategy. As a result, 759 mg of selectively enriched bufadienolide was obtained from 2 g of crude extract and the total content of five targets was increased from 14.64 to 83%. A total of 31 mg of marinobufagin‐3‐adipoyl‐l‐arginine, 42 mg of telocinobufagin‐3‐pimeloyl‐l‐arginine, 51 mg of telocinobufagin‐3‐suberoyl‐l‐arginine, 132 mg of marinobufagin‐3‐suberoyl‐l‐arginine, and 57 mg of bufalin‐3‐suberoyl‐l‐arginine were all simultaneously separated from 500 mg of selectively enriched sample, with the purity of 92.4, 97.5, 90.3, 92.1, and 92.8%, respectively.

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“…Bufalin-3-suberoyl-l-arginine(E): 1 Table S1. Those data were in agreement with earlier published data for Bufalin-3-suberoyl-l-arginine [27,41].…”

Section: Ta B L E 2 Thesupporting

confidence: 93%

An efficient strategy based on liquid‐liquid extraction and pH‐zone‐refining counter‐current chromatography for selective enrichment, separation, and purification of alkaloids and organic Acids from natural products

et al. 2020

J of Separation Science

View full text Add to dashboard Cite

show abstract

Ab initio conformational analysis of marinobufagenin molecule and molecular targets of the action of cardiotonic steroids

Cited by 1 publication

References 14 publications

An efficient strategy based on liquid‐liquid extraction and pH‐zone‐refining counter‐current chromatography for selective enrichment, separation, and purification of alkaloids and organic Acids from natural products

An efficient strategy based on liquid‐liquid extraction and pH‐zone‐refining counter‐current chromatography for selective enrichment, separation, and purification of alkaloids and organic Acids from natural products

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