AA-Dutp-Cy3 a Novel Fluorescent Labeling Agent for Nucleotides

Abstract

A bioassay was developed to provide a process for the synthesis of cyanine dye labeled base modified nucleoside triphosphates analogues as a novel labeling agent for biomolecules. It is also to provide a low cost process and more specifically relates to a process for the synthesis of cyanine fluorophore -base modified nucleoside triphosphates analogues as well as for efficient and robust labeling of RNA and cDNA as hybridization probe in microarray detection and analysis.

Engineering of functional nanosnowflakes from gold nanocarriers capped with amino‐modified DNA oligonucleotides

“…(5-aminoallyl-2 0 -deoxyuridine 5 0 -triphosphate) might be incorporated into the oligonucleotide ligands via conventional polymerase chain reaction (PCR) technique. It is commonly used in the post-labeling techniques of nucleic acids by fluorescence detection (Chaudhuri, 2014;Tjong et al, 2013) but the benefit of this unnatural base in the proposed design is that it might provide nuclease resistance, and the positive charged amino group are forming electrostatic interaction with the negatively charged phosphate groups from the sugar-phosphate backbone. The resulted structural assembly contained amine-modified oligonucleotides attached on gold nanocarrier surface by the formed Au-sulfur bonds are stable at relevant pH and physiological ionic strength of solution (Badia et al, 1997;Reimers et al, 2016).…”