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Abstract

Trypanosoma evansi positive blood samples were collected from the dogs, multiplied in the wistar rats. Whole cell lysate antigen of Trypanosoma evansi parasites was prepared for further study by using the host cell free Trypanosoma evansi parasites. Study revealed a total of 13 polypeptide bands with relative molecular weight ranging from 25 to 82 kDa, when resolved in 10% sodium dodecyl sulphate polyacrylamide gel ectrophoresis and stained with Coomassie brilliant blue. Four major polypeptides of relative molecular weight of 33, 36, 41, 43 kDa and remaining nine polypeptides were minor appeared at molecular weight of 25, 28, 38, 59, 66, 70, 72, 80, 82 kDa. There is no variation in the antigenic profile of all the samples which were collected from the Rayalaseema region of Andhra Pradesh.