A549 cells adapted to high nitric oxide show reduced surface CEACAM expression and altered adhesion and migration properties

Abstract

The migration and adhesion properties of tumors affect their metastatic rate. In the present study, we investigated carcinoembryonic antigen-related cell adhesion molecule (CEACAM) 1, 5, and 6 expression in high nitric oxide (HNO)-adapted lung cancer cells compared to parent cells. We observed high transcript levels of CEACAM 1 (4S, 4L), CEACAM 5, and CEACAM 6 in HNO cells compared to parent cells. However, the surface expression was low in HNO cells. Interestingly, the intracellular protein levels were high for these three CEACAMs. We confirmed these results with immunohistochemical experiments. Further, the adhesion and migration assays showed reduced clumping in HNO-adapted A549 (A549-HNO) cells and faster migration rates, respectively. These results document the altered adhesion and migration properties of cells adapted to HNO. Further, our studies also indicate a dynamic regulation of CEACAM protein expression and surface transport in HNO cells.

Characterization of a member of the CEACAM protein family as a novel marker of proton pump-rich ionocytes on the zebrafish epidermis

“…In the tumor microenvironment, these properties are thought to shape the communication between cancer, stromal and immune cells, thereby influencing numerous cellular processes during cancer progression such as cell proliferation, motility, angiogenesis, metastasis or immune responses [2,16]. In both normal and cancer tissues, intercellular contacts mediated by epithelial members of the CEACAM family have also been implicated in apoptosis [24], cell migration [25,26], vascular remodeling [27] and morphogenesis. Interestingly, a number of CEACAMs present in the digestive tract are also used as anchoring points by several intestinal pathogens including pathogenic Escherichia coli [28,29], Moraxella catarrhalis [30,31], Haemophilus influenzae [31], Helicobacter pylori [32] and several neisserial strains [33].…”

Therapeutic Effect of pHLIP-mediated CEACAM6 Gene Silencing in Lung Adenocarcinoma

“…In NSCLC, lung adenocarcinomas express higher levels of CEACAM6 protein than other histologic subtypes. Although other CEACAM family proteins, such as CEACAM1 and CEACAM5, are known to be dysregulated in lung adenocarcinoma as well, CEACAM6 is more highly expressed than CEACAM5 and is associated with poor clinical outcomes among lung adenocarcinoma patients 3,7,13–15,18,19 . These data support a critical role of CEACAM6 in lung adenocarcinoma and suggest that it is an attractive therapeutic target.…”