Abstract:Currently used elaborate technologies for the detection of bacteria can be improved in regard to their time consumption, labor intensity, accuracy and reproducibility. Well-known electrical measurement methods might connect highly sensitive sensing systems with biological requirements. The development of modified sensor surfaces with self-assembled monolayers (SAMs) from functionalized porphyrin for bacteria trapping can lead to a highly sensitive sensor for bacteria detection. Different A2BC-type porphyrin st… Show more
“…Also the ability of the porphyrin molecule (Fig. 1 ) to link bacteria cells on the electrode’s surface was verified by fluorescence lifetime imaging microscopy (FLIM) 17 . This work focuses on the detection of bacteria by the developed EGFET.…”
Section: Resultsmentioning
confidence: 93%
“…Gold surfaces were functionalized with a modified porphyrin, shown in Fig. 1 , by an acid-promoted method, as it is described by Neumann et al 17 , 21 . Figure 1 Modified porphyrin for functionalization purposes.…”
Section: Experimental Methods and Materialsmentioning
confidence: 99%
“…Previous publications modified electrode surfaces with different bioreceptors as antibodies, aptamers, or bacteriophages 14 – 16 . As alternative, bio-inspired porphyrins are of great interest due to their biological compatibility, their fluorescence in the visible range, their chemical stability under ambient condition, and their simple chemical accessibility 17 . So far, sensor systems have been functionalized by porphyrins to detect for example magnesium (II) ions, histidine, or DNA 18 – 20 .…”
Traditional sensing technologies have drawbacks as they are time-consuming, cost-intensive, and do not attain the required accuracy and reproducibility. Therefore, new methods of measurements are necessary to improve the detection of bacteria. Well-established electrical measurement methods can connect high sensitive sensing systems with biological requirements. One approach is to functionalize an extended-gate field-effect transistor’s (EGFET) sensing area with modified porphyrins containing two different linkers. One linker connects the electrode surface with the porphyrin. The other linker bonds bacteria on the functional layer through a specific peptide chain. The negative charge on the surface of the cells regulates the surface potential which has an impact on the electrical behavior of the EGFET. The attendance of attached bacteria on the functionalized sensing area could successfully be detected.
“…Also the ability of the porphyrin molecule (Fig. 1 ) to link bacteria cells on the electrode’s surface was verified by fluorescence lifetime imaging microscopy (FLIM) 17 . This work focuses on the detection of bacteria by the developed EGFET.…”
Section: Resultsmentioning
confidence: 93%
“…Gold surfaces were functionalized with a modified porphyrin, shown in Fig. 1 , by an acid-promoted method, as it is described by Neumann et al 17 , 21 . Figure 1 Modified porphyrin for functionalization purposes.…”
Section: Experimental Methods and Materialsmentioning
confidence: 99%
“…Previous publications modified electrode surfaces with different bioreceptors as antibodies, aptamers, or bacteriophages 14 – 16 . As alternative, bio-inspired porphyrins are of great interest due to their biological compatibility, their fluorescence in the visible range, their chemical stability under ambient condition, and their simple chemical accessibility 17 . So far, sensor systems have been functionalized by porphyrins to detect for example magnesium (II) ions, histidine, or DNA 18 – 20 .…”
Traditional sensing technologies have drawbacks as they are time-consuming, cost-intensive, and do not attain the required accuracy and reproducibility. Therefore, new methods of measurements are necessary to improve the detection of bacteria. Well-established electrical measurement methods can connect high sensitive sensing systems with biological requirements. One approach is to functionalize an extended-gate field-effect transistor’s (EGFET) sensing area with modified porphyrins containing two different linkers. One linker connects the electrode surface with the porphyrin. The other linker bonds bacteria on the functional layer through a specific peptide chain. The negative charge on the surface of the cells regulates the surface potential which has an impact on the electrical behavior of the EGFET. The attendance of attached bacteria on the functionalized sensing area could successfully be detected.
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