A20 Inhibits LPS-Induced Inflammation by Regulating TRAF6 Polyubiquitination in Rainbow Trout

Jang, Ju Hye; Kim, Hyun; Jung, In Young; Cho, Ju Hyun

doi:10.3390/ijms22189801

Cited by 9 publications

(3 citation statements)

References 54 publications

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“…The supernatants containing secreted proteins were collected and filter-sterilized using a 0.22 µm filter. Samples were separated by SDS-PAGE using the PeptiGel TM I Peptide PAGE Analysis Kit (ELPIS-BIOTECH, Daejeon, Republic of Korea), and the immunoblotting assay was conducted as previously described [36]. The expressed AMPs were detected by incubation with a 6 × His-tag monoclonal antibody (Invitrogen) and a goat anti-mouse IgG (H + L) HRP-conjugated antibody (GenDEPOT, Katy, TX, USA).…”

Section: Characterization Of Amp Secretion Module For the Inducible P...mentioning

confidence: 99%

Novel Genetically Engineered Probiotics for Targeted Elimination of Pseudomonas aeruginosa in Intestinal Colonization

Kim,

Jang,

Jung

et al. 2023

Biomedicines

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The intestinal carriage rates of Pseudomonas aeruginosa are notably elevated in immunosuppressed individuals and hospitalized patients, increasing the risk of infection and antibiotic-associated diarrhea. A potential solution to this issue lies in autonomous antibacterial therapy, remaining inactive until a pathogen is detected, and releasing antibacterial compounds on demand to eliminate the pathogen. This study focuses on the development of genetically engineered probiotics capable of detecting and eradicating P. aeruginosa by producing and secreting PA2-GNU7, a P. aeruginosa-selective antimicrobial peptide (AMP), triggered by the presence of P. aeruginosa quorum-sensing molecule N-(3-oxododecanoyl)-L-homoserine lactone (3OC12HSL). To achieve this goal, plasmid-based systems were constructed to produce AMPs in response to 3OC12HSL and secrete them into the extracellular medium using either the microcin V secretion system or YebF as a carrier protein. Following the transfer of these plasmid-based systems to Escherichia coli Nissle 1917 (EcN), we successfully demonstrated the ability of the engineered EcN to express and secrete PA2-GNU7, leading to the inhibition of P. aeruginosa growth in vitro. In addition, in a mouse model of intestinal P. aeruginosa colonization, the administration of engineered EcN resulted in reduced levels of P. aeruginosa in both the feces and the colon. These findings suggest that engineered EcN holds promise as a potential option for combating intestinal P. aeruginosa colonization, thus mitigating the risk of future endogenous infections in vulnerable patients.

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Section: Characterization Of Amp Secretion Module For the Inducible P...mentioning

confidence: 99%

Novel Genetically Engineered Probiotics for Targeted Elimination of Pseudomonas aeruginosa in Intestinal Colonization

Kim,

Jang,

Jung

et al. 2023

Biomedicines

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“…The A20/AN1 zinc-finger protein's main role is to regulate the immune response. It also acts as a de-ubiquitinating enzyme, and is involved in controlling fundamental cellular activities [72][73][74]. Pentraxin, which functions as a soluble patternrecognition molecule, was also identified.…”

Section: Cellular Immunological Processesmentioning

confidence: 99%

Qualitative Shotgun Proteomics Strategy for Protein Expression Profiling of Fish Otoliths

Rideout,

Youssef,

Adamack

et al. 2023

BioChem

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Despite decades of research on fish otoliths and their capacity to serve as biochronological recorders, much remains unknown about their protein composition, the mechanisms by which proteins are incorporated into the otolith matrix, or the potential for using otolith proteins to provide insight into aspects of fish life history. We examined the protein composition of Atlantic cod (Gadus morhua) otoliths using a state-of-the-art shotgun proteomics approach with liquid chromatography coupled to an electrospray ionization-orbitrap tandem mass spectrometer. In addition to previously known otolith matrix proteins, we discovered over 2000 proteins not previously identified in cod otoliths and more than 1500 proteins not previously identified in any fish otoliths. These included three novel proteins (Somatolactin, F-actin-capping protein subunit beta, Annexin) primarily involved in binding calcium ions and likely mediating crystal nucleation. However, most of the otolith proteins were not necessarily related to otolith formation but rather to other aspects of fish physiology. For example, we identified sex-related biomarkers for males (SPATA6 protein) and females (Vitellogenin-2-like protein). We highlight some noteworthy classes of proteins having diverse functions; however, the primary goal here is not to discuss each protein separately. The number and diverse roles of the proteins discovered in the otoliths suggest that proteomics could reveal critical life history information from archived otolith collections that could be invaluable for understanding aspects of fish biology and population ecology. This proof-of-concept methodology paper provides a novel methodology whereby otolith proteomics can be further explored.

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“…IRAK-M is another IRAK inhibitor, which belongs to the IRAK kinase family, but cannot induce NF-κB activation [ 82 ]. In addition to the inhibitors targeting IRAKs, proteins binding to TRAF6 [ 83 ], namely A20 [ 84 ] (also called TNF- α induced protein 3, TNFAIP3), NLR family member X1 (NLRX1) [ 85 , 86 ], and the cylindromatosis protein (CYLD), have also been shown to be negative regulators of TLR2 signaling [ 87 , 88 ]. Furthermore, A20 and NLRX1 can also block the activation of the IKK complex [ 89 ].…”

Section: Regulation Of Tlr2 Signalingmentioning

confidence: 99%

The Role of TLR2 in Infectious Diseases Caused by Mycobacteria: From Cell Biology to Therapeutic Target

Spaink

2022

Biology

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Innate immunity is considered the first line of defense against microbial invasion, and its dysregulation can increase the susceptibility of hosts to infections by invading pathogens. Host cells rely on pattern recognition receptors (PRRs) to recognize invading pathogens and initiate protective innate immune responses. Toll-like receptor 2 (TLR2) is believed to be among the most important Toll-like receptors for defense against mycobacterial infection. TLR2 has been reported to have very broad functions in infectious diseases and also in other diseases, such as chronic and acute inflammatory diseases, cancers, and even metabolic disorders. However, TLR2 has an unclear dual role in both the activation and suppression of innate immune responses. Moreover, in some studies, the function of TLR2 was shown to be controversial, and therefore its role in several diseases is still inconclusive. Therefore, although TLR2 has been shown to have an important function in innate immunity, its usefulness as a therapeutic target in clinical application is still uncertain. In this literature review, we summarize the knowledge of the functions of TLR2 in host–mycobacterial interactions, discuss controversial results, and suggest possibilities for future research.

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A20 Inhibits LPS-Induced Inflammation by Regulating TRAF6 Polyubiquitination in Rainbow Trout

Cited by 9 publications

References 54 publications

Novel Genetically Engineered Probiotics for Targeted Elimination of Pseudomonas aeruginosa in Intestinal Colonization

Novel Genetically Engineered Probiotics for Targeted Elimination of Pseudomonas aeruginosa in Intestinal Colonization

Qualitative Shotgun Proteomics Strategy for Protein Expression Profiling of Fish Otoliths

The Role of TLR2 in Infectious Diseases Caused by Mycobacteria: From Cell Biology to Therapeutic Target

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