A β‐mannanase from Paenibacillus sp.: Optimization of production and its possible prebiotic potential

Abstract

A thermotolerant bacterium Paenibacillus thiaminolyticus with an ability to produce extracellular β-mannanase was isolated from a soil sample. Bacterium produced 45 U/mL β-mannanase at 50 °C. The culture conditions for high-level production of β-mannanase were optimized. Optimized MS medium [wheat bran 2% (w/v), ammonium sulfate 0.3% (w/v), yeast extract, and peptone (0.025% each) pH 6.5] was inoculated with 2% of 16 H old culture. The culture was incubated at 50 °C for 48 H resulting in 24-folds higher β-mannanase production (1,100 ± 50 U/mL). Optimum pH and temperature for enzyme activity of the crude enzyme was 6.0 and 60 °C, respectively. The enzyme demonstrated 65% relative enzyme activity at 37 °C. The hydrolytic activity of the crude enzymatic preparation was assessed on various agro residues. Thin-layer chromatographic analysis showed that the enzyme activity to saccharify heteromannans resulted in production of a mixture of manno-oligosaccharides (MOS) and enzyme exhibited classic endo-activity. To evaluate the possible prebiotic potential of the MOS thus obtained, initial screening for their ability to support the growth of probiotics was carried out by the pure culture method. Bifidobacterium and Lactobacillus sp. responded positively to the addition of enzymatically derived oligosaccharides and their numbers increased significantly.

Purification and characterization of β-mannanase from Aspergillus terreus and its applicability in depolymerization of mannans and saccharification of lignocellulosic biomass

“…MOS are able to interfere in bacterial attachment (of Salmonella and E. coli ). In the intestinal tract, however, these oligosaccharides selectively promote the growth of beneficial bacteria, especially Lactobacillus and Bifidobacterium (Chauhan et al 2014 ; Dhawan et al 2015 ). Thus, the production of oligosaccharides with DP 3–4 makes A. terreus β-mannanase a good candidate for potential application in the feed industry.…”

Man/Cel5B, a Bifunctional Enzyme Having the Highest Mannanase Activity in the Hyperthermic Environment

“…β-mannanase is extensively used in the paper/pulp, food, and feed industries, particularly in poultry feeds and prebiotic food supplements to decrease the immunogenic effect of mannan polymers, clarify fruit juices, and extract of oil from copra and detergent (Kaira et al, 2016;Singh et al, 2019). In these industries, thermostable enzymes are preferentially used (Dhawan et al, 2016;Li and Nie, 2016) due to their robustness and enhanced rate of hydrolysis (Katsimpouras et al, 2016;Niu et al, 2017). Moreover, thermozymes offer unique features such as high pH, solvent concentrations (Liu et al, 2019), and resistance to chemical denaturation (Akram et al, 2018), and they could reduce contamination from unwanted microbes (Ebaid et al, 2019).…”

Experimental design of response surface methodology used for utilisation of palm kernel cake as solid substrate for optimised production of fungal mannanase

“…Mannose-free manno-oligosaccharides (MOS) are desirable as prebiotics in nutraceutical industries. Recently, Chauhan et al (2014) and Dhawan et al (2015) have demonstrated the role of MOS as prebiotic in supporting growth of probiotics ( Lactobacillus and Bifidobacterium ) and inhibiting the growth of pathogen ( Salmonella enterica ). The focus of this study is the optimised production of β-mannanase on a solid substrate (PKC), a by-product of palm oil industry, and enzymatic hydrolysis of mannan (locust bean gum and konjac gum) for the generation of MOS.…”