A zinc finger-encoding gene coregulated with c-fos during growth and differentiation, and after cellular depolarization

Sukhatme, Vikas P.; Cao, Xinmin; Chang, Louise; Tsai‐Morris, Chon‐Hwa; Stamenkovich, Dorothy; Ferreira, P. C. P.; Cohen, Donna R.; Edwards, S.; Shows, Thomas B.; Curran, Tom; Beau, Michelle M. Le; Adamson, Eileen D.

doi:10.1016/0092-8674(88)90485-0

Cited by 1,201 publications

(671 citation statements)

References 36 publications

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“…Expression of Egr1 is rapidly induced by mitogens, insulin and glucose in different cells, including beta cells [19,20,34,35]. In our experiments, the rapid and transient induction of EGR1 protein in response to exendin-4 ( Fig.…”

Section: Discussionsupporting

confidence: 52%

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Upregulation of rat Ccnd1 gene by exendin-4 in pancreatic beta cell line INS-1: interaction of early growth response-1 with cis-regulatory element

Kang

Kim

et al. 2006

Diabetologia

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Aims/hypothesis: The aim of this study was to investigate the effect of exendin-4 on the expression of cyclin D1 gene (Ccnd1), which is critical in regulating the progression of the cell cycle in INS-1 cells. Materials and methods: INS-1 cells were stimulated with exendin-4 (10 nmol/l). Transient transfection and luciferase reporter assays were performed to measure promoter activities of rat Ccnd1. Electrophoretic mobility shift and chromatin immunoprecipitation assays were used to examine the binding of transcription factors to sites responsive to exendin-4 in vitro and in vivo, respectively. Results: Exendin-4 increased both Ccnd1 mRNA and its protein levels in a time-dependent manner. The region from −174 to +130 of the promoter was found to contain cis-regulatory elements responsible for exendin-4-mediated gene induction. Early growth response-1 (EGR1) protein was bound to the region from −153 to −134, which includes the putative EGR1 binding site (5′-CACCCCCGC-3′). Moreover, exendin-4 recruited EGR1 protein to the promoter in vivo. Conclusions/ interpretation: These findings suggest that exendin-4 activates Ccnd1 transcription through induction of EGR1 binding to a cis-regulatory element between −153 and −134 on the rat Ccnd1 promoter. These results provide an important indication that exendin-4 is a growth factor regulating beta cell proliferation.

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Section: Discussionsupporting

confidence: 52%

“…8a). EGR1 is an immediateearly response protein that is newly synthesised upon stimulation [19,20]. Thus, for the interaction of EGR1 with the Ccnd1 promoter, de novo EGR1 protein synthesis is required.…”

Section: Effect Of Exendin-4 On Egr1 Production In Ins-1 Cells and Prmentioning

confidence: 99%

Upregulation of rat Ccnd1 gene by exendin-4 in pancreatic beta cell line INS-1: interaction of early growth response-1 with cis-regulatory element

Kang

Kim

et al. 2006

Diabetologia

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“…[1][2][3] Ionizing radiation (IR) as well as DNA-damaging chemotherapeutic agents readily induce the Egr-1 promoter via the CArG elements. [4][5][6][7] This regulation is exploited in the replication-deficient adenoviral gene therapy vector Ad.Egr.TNF (TNFerade Biologic, GenVec Inc., Gaithersburg, MD), in which the pro-apoptotic cytokine tumor necrosis factor a (TNF-a), under control of CArG elements, is induced by radio-and/or chemotherapy.…”

Section: Introductionmentioning

confidence: 99%

Resveratrol is an effective inducer of CArG-driven TNF-α gene therapy

et al. 2007

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We report the anticarcinogenic, anti-aging polyphenol resveratrol activates the radio-and chemo-inducible cancer gene therapy vector Ad.Egr.TNF, a replication-deficient adenovirus that expresses human tumor necrosis factor a (TNF-a) under control of the Egr-1 promoter. Like ionizing radiation or chemotherapeutic agents previously shown to activate Ad.Egr.TNF, resveratrol also induces Egr-1 expression from its chromosomal locus with a possible role for Egr-1 promoter CC(A þ T)richGG sequences in the expression of TNF-a. Resveratrol induction of TNF-a in Ad.Egr.TNF-infected tumor xenografts demonstrated antitumor response in human and rat tumor models comparable to that of radio-or chemotherapy-induced TNF-a. Although sirtuins are known targets of resveratrol, in vitro inhibition of SIRT1 activity did not abrogate resveratrol induction of Egr-1 expression. This suggests that SIRT1 is not essential to mediate resveratrol induction of Egr-1. Nevertheless, control of transgene expression via resveratrol activation of Egr-1 may extend use of Ad.Egr.TNF to patients intolerant of radiation or cytotoxic therapy and offer a novel tool for development of other inducible gene therapies.

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“…Basically, a transient and rapid activation of these genes occurs within the first few hours of cell activation and does not require de novo protein synthesis. Several immediate-early genes encoding secretory proteins, structural proteins such as actin and transcription factors such as Jun [2], Fos [3] and zinc-finger proteins [4] are probably involved in the regulation of expression of genes at the later point of the cell cycle (called delayed genes). As such, they may have a crucial role in the cell cycle progression and cell growth.…”

Section: Introductionmentioning

confidence: 99%

NOR‐2 (neuron‐derived orphan receptor), a brain zinc finger protein, is highly induced during liver regeneration

Petropoulos¹,

Part²,

Ochoa³

et al. 1995

FEBS Letters

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~,bstraet Zinc-finger proteins are involved in several cellular processes. Some of these proteins are implicated in the primary cellular response in regenerating liver and mitogen-stimulated cells. Using a rat cDNA brain library, we have isolated a clone designated NOR-2, encoding a protein containing two zinc-finger motifs and whose expression is highly induced during G0/GI transition. We analysed the expression of NOR-2 mRNAs during early growth in regenerating liver and in both insulin-stimulated H4-11 cells and pheochromocytoma-derived cell line PCI2 treated by NGF. In these systems, there is an early, rapid and transient accumulation of NOR-2 mRNAs. The induction of NOR-2 mRNAs does not require de novo protein synthesis, since it is not prevented by cycloheximide treatment. Mobility shift assays show that NOR-2 protein binds to NBRE, a target sequence for r-NGFI-B family. Structurally, NOR-2 is closely related to the recently identified NOR-I factor. Therefore, like NOR-I, NOR-2 belongs to the r-NGFI-B sub-family of nuclear receptors superfamily.

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A zinc finger-encoding gene coregulated with c-fos during growth and differentiation, and after cellular depolarization

Cited by 1,201 publications

References 36 publications

Upregulation of rat Ccnd1 gene by exendin-4 in pancreatic beta cell line INS-1: interaction of early growth response-1 with cis-regulatory element

Upregulation of rat Ccnd1 gene by exendin-4 in pancreatic beta cell line INS-1: interaction of early growth response-1 with cis-regulatory element

Resveratrol is an effective inducer of CArG-driven TNF-α gene therapy

NOR‐2 (neuron‐derived orphan receptor), a brain zinc finger protein, is highly induced during liver regeneration

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