A zinc-dependent peptidase in prostatic organelles present in seminal plasma

Laurell, C.‐B.; Weiber, Håkan; Ohlsson, Kjell; Rannevik, Gunnar

doi:10.1016/0009-8981(82)90032-8

Cited by 67 publications

(39 citation statements)

References 13 publications

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“…The aminopeptidase assay was performed according to Laurel1 et al [8]. The protein concentration was estimated according to Lowry et al [9] using a commercial human serum (Seronorm, Nycomed AS, Oslo, Norway) as a standard.…”

Section: Methodsmentioning

confidence: 99%

Motility Stimulant Effects of Prostasome Inclusion in Swim-Up Medium on Cryopreserved Human Spermatozoa

Carlsson

Ronquist

Stridsberg

et al. 1997

Archives of Andrology

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Section: Methodsmentioning

confidence: 99%

Motility Stimulant Effects of Prostasome Inclusion in Swim-Up Medium on Cryopreserved Human Spermatozoa

Carlsson

Ronquist

Stridsberg

et al. 1997

Archives of Andrology

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“…Fractions of 2.5 ml were collected. The presence of prostasomes in fractions was demonstrated by assay for amino-peptidase activity, 16 and spectrophotometry at 280 nm. 17 Prostasome containing fractions were pooled, and the protein concentration measured.…”

Section: Prostasomesmentioning

confidence: 99%

Seminal prostasomes inhibit the angiogenesis activity of rat aortic rings

Delves

Goyal

Lwaleed³

et al. 2006

Prostate Cancer Prostatic Dis

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Prostasomes are organelles secreted by prostatic epithelial cells, and are believed to have a role in fertility and prostatic disease. They are known to influence sperm motility and the acrosome reaction, and are thought to have a role in cell transformation, immunosuppression, proliferation, facilitation of local invasion, and angiogenesis. Previously, we have demonstrated the inhibitory effect of prostasomes derived from human semen on angiogenesis using HUVEC cells grown on matrigel. In this study, we use the rat aortic ring assay system, arguably a closer reflection of the in vivo situation. Quantification was by a spectrophotometric method, and underlying mechanisms assessed. Prostasomes demonstrated a clear inhibition of angiogenesis, and this effect persisted after heat treatment of prostasomes to denature protein. This fits with other known effects of prostasomes known to be due to the membrane lipid component, which is unusually high in sphingomyelin and cholesterol.

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“…Fractions of 2.5 ml were collected. The presence of prostasomes in fractions was demonstrated by assay for amino-peptidase activity, 15 and spectrophotometry at 280 nm. 16 Prostasome containing fractions were pooled, and the protein concentration measured.…”

Section: Preparation Of Prostasomesmentioning

confidence: 99%

In vitro inhibition of angiogenesis by prostasomes

Delves

Stewart²,

Lwaleed

et al. 2005

Prostate Cancer Prostatic Dis

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Prostasomes are biologically active organelles that are secreted by human prostate epithelial cells, and it is believed that they have a role in prostatic disease. We studied the effect of prostasomes on the human umbilical vein endothelial cell (HUVEC)/Matrigel model of angiogenesis, and the association of labelled prostasomes with HUVECs. The growth inhibitory effect of prostasomes on HUVECs was assayed by spectrophotometric measurement of residual biomass. Preparations of HUVECs on a Matrigel base were exposed to prostasomes, and the development of capillary-like networks was quantified. Prostasomes were labelled with PKH-26, and cultured with HUVECs. Prostasomes were not shown to have a significant effect on HUVEC survival. Angiogenesis assays showed inhibition. The PKH-26-labelled particles were shown to have adhered to the HUVECs. This study adds the inhibition of an in vitro correlate of angiogenesis to the known actions of prostasomes.

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A zinc-dependent peptidase in prostatic organelles present in seminal plasma

Cited by 67 publications

References 13 publications

Motility Stimulant Effects of Prostasome Inclusion in Swim-Up Medium on Cryopreserved Human Spermatozoa

Motility Stimulant Effects of Prostasome Inclusion in Swim-Up Medium on Cryopreserved Human Spermatozoa

Seminal prostasomes inhibit the angiogenesis activity of rat aortic rings

In vitro inhibition of angiogenesis by prostasomes

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