A zinc complex that suppresses the expression of a reactive sulfur species-producing enzyme, cystathionine γ-lyase, in cultured vascular endothelial cells

Abstract

Persulfide species present in mammalian cells play important toxicological roles against oxidative stress and methylmercury. Cystathionine γ-lyase (CSE) and cystathionine β-synthase (CBS) are enzymes that produce persulfide species using cysteine as a substrate in the presence of vitamin B 6. However, little is known about the regulatory mechanisms underlying the expression of these enzymes. In the present study, we searched for molecular probes from a library of 27 zinc complexes to analyze the mechanisms in vascular endothelial cells. We found dichloro(1,10-phenanthroline)zinc(II), termed Zn11, as a zinc complex that suppressed endothelial CSE expression without any change in CBS expression. This zinc complex can be used as molecular probes to analyze the regulation underlying endothelial CSE expression.

Cell density-dependent accumulation of low polarity gold nanocluster in cultured vascular endothelial cells

“…As the creation of such nanomaterials has become widespread, the toxicity and distribution of the molecules have also been studied (Perrault et al, 2009;Chou and Chan, 2012;Jiang et al, 2015;Foroozandeh and Aziz, 2018). We also reported the cytotoxicity and function of organic-inorganic hybrid molecules -metal complexes and organometallic compounds-as tools for analyzing biological systems of vascular endothelial cells at different cell densities (Fujie et al, 2016(Fujie et al, , 2019(Fujie et al, , 2020Hara et al, 2017Hara et al, , 2018aHara et al, , 2019Hara et al, , 2020Takahashi et al, 2018). However, because these hybrid molecules are generally electrophilic, the response of these cells against metal compounds that lack electrophilicity is not well understood.…”

Cadmium induces plasminogen activator inhibitor-1 via Smad2/3 signaling pathway in human endothelial EA.hy926 cells

“…Smad2/3 and β-actin proteins were separated by SDS-PAGE, and western blotting was performed as described previously (Takahashi et al, 2018). Immunoreactive bands were visualized using Chemi-Lumi One Super western blot detection reagent and scanned using an Amersham Imager 600 (GE Healthcare).…”