A Zfp609 circular RNA regulates myoblast differentiation by sponging miR-194-5p

Wang, Yanhong; Li, Menglu; Wang, Yanhuan; Liu, Jia; Zhang, MoLan; Fang, Xingtang; Chen, Hong; Zhang, Chunlei

doi:10.1016/j.ijbiomac.2018.09.039

Cited by 77 publications

(68 citation statements)

References 34 publications

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“…Circ-ZNF609 was shown to be associated with polysomes, although at low levels, and to be translated into a protein in a splicing-dependent and cap-independent manner when ectopically expressed [ 43 ]. Interestingly, Zfp609 , the murine homolog of ZNF609, contains miRNA binding sites and was recently shown to inhibit the expression of myogenic transcription factors in C2C12 myoblasts possibly by sponging miR-194-5p [ 69 ] ( Table 2 ).…”

Section: Circular Rnas In Myogenesismentioning

confidence: 99%

Circular RNAs in Muscle Function and Disease

Greco

Cardinali

Falcone

et al. 2018

IJMS

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Circular RNAs (circRNAs) are a class of RNA produced during pre-mRNA splicing that are emerging as new members of the gene regulatory network. In addition to being spliced in a linear fashion, exons of pre-mRNAs can be circularized by use of the 3′ acceptor splice site of upstream exons, leading to the formation of circular RNA species. In this way, genetic information can be re-organized, increasing gene expression potential. Expression of circRNAs is developmentally regulated, tissue and cell-type specific, and shared across eukaryotes. The importance of circRNAs in gene regulation is now beginning to be recognized and some putative functions have been assigned to them, such as the sequestration of microRNAs or proteins, the modulation of transcription, the interference with splicing, and translation of small proteins. In accordance with an important role in normal cell biology, circRNA deregulation has been reported to be associated with diseases. Recent evidence demonstrated that circRNAs are highly expressed in striated muscle tissue, both skeletal and cardiac, that is also one of the body tissue showing the highest levels of alternative splicing. Moreover, initial studies revealed altered circRNA expression in diseases involving striated muscle, suggesting important functions of these molecules in the pathogenetic mechanisms of both heart and skeletal muscle diseases. The recent findings in this field will be described and discussed.

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Section: Circular Rnas In Myogenesismentioning

confidence: 99%

Circular RNAs in Muscle Function and Disease

Greco

Cardinali

Falcone

et al. 2018

IJMS

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“…There con rmed the role of circFUT10 [28], circSVIL [8], circFGFR4 [25], circFGFR2 [42], and circSNX29 [29] in the enhance of myogenesis through positively regulated myoblast differentiation. Moreover, it was found that circLMO7 [27], circZNF609 [26], negatively regulated the myoblast differentiation.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, it was also investigated that the circZfp609 sponges miR-194-5p to inhibit myogenic differentiation in C2C12 [26]. Another research also found that circLMO7 overexpression promotes proliferation and inhibits bovine primary myoblasts differentiation by sponging miR-378a-3p [27].…”

Section: Introductionmentioning

confidence: 99%

Circular RNA circMYL1 inhibit proliferation and promote differentiation of myoblasts by sponging miR-2400

Elnour

Wang

Zhansaya

et al. 2020

Preprint

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Background Circular RNAs (circRNAs) are a class of endogenous non-coding RNAs (ncRNAs) involved in regulating skeletal muscle development by sponging microRNAs (miRNAs). This study aimed to define the circMYL1 molecular mechanisms in the regulation of bovine myogenesis and to disclose its regulatory mechanism through miR-2400 interaction. Methods The potential role of circMYL1 was identified in the proliferation of bovine myoblast through mRNA and protein expression of proliferation marker genes (PCNA, CyclinD1, and CDK2), cell counting kit-8 (CCK8) assay, flow cytometry analysis, and EdU assay. Analysis of the expression of differentiation marker genes (MyoD, MyoG, and MYH2) and immunofluorescence of Myosin heavy chain (MyHC) was used to assess cell differentiation. Results The proliferation analysis revealed that circMYL1 inhibited the proliferation of bovine primary myoblast. Furthermore, the differentiation analysis demonstrated that circMYL1 promoted the differentiation of bovine primary myoblast. The luciferase screening and RNA immunoprecipitation (RIP) assays found that circMYL1 could have interaction with miR-2400. Additionally, we demonstrated that miR-2400 promoted proliferation and inhibited differentiation of bovine primary myoblast, while circMYL1 may eliminate the effects of miR-2400, as showed by rescue experiments. Conclusions Our results revealed that a novel circular RNA of circMYL1 could inhibit proliferation and promote differentiation of myoblast by sponging miR-2400.

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“…[95][96][97][98][99] Circ-ZNF609 promotes human myoblast proliferation through an unknown mechanism 95 ; however, circZfp609, which is the gene symbol of mouse ZNF609, sponges miR-194-5p to sequester its inhibition of BCL2-associated transcription factor 1 (BCLAF1) and repress myogenic differentiation by affecting the expression of Myf5 and MyoG. [95][96][97][98][99] Circ-ZNF609 promotes human myoblast proliferation through an unknown mechanism 95 ; however, circZfp609, which is the gene symbol of mouse ZNF609, sponges miR-194-5p to sequester its inhibition of BCL2-associated transcription factor 1 (BCLAF1) and repress myogenic differentiation by affecting the expression of Myf5 and MyoG.…”

Section: Muscle Developmentmentioning

confidence: 99%

“…[95][96][97][98][99] Circ-ZNF609 promotes human myoblast proliferation through an unknown mechanism 95 ; however, circZfp609, which is the gene symbol of mouse ZNF609, sponges miR-194-5p to sequester its inhibition of BCL2-associated transcription factor 1 (BCLAF1) and repress myogenic differentiation by affecting the expression of Myf5 and MyoG. 96 Additionally, CircLMO7 inhibits differentiation, promotes the proliferation of primary bovine myoblasts and protects them from apoptosis. It appears to function as a ceRNA for miR-378a-3p by targeting histone acetylation enzyme 4 (HDAC4).…”

Section: Muscle Developmentmentioning

confidence: 99%

Roles of lncRNAs and circRNAs in regulating skeletal muscle development

et al. 2019

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The multistep biological process of myogenesis is regulated by a variety of myoblast regulators, such as myogenic differentiation antigen, myogenin, myogenic regulatory factor, myocyte enhancer factor2A-D and myosin heavy chain. Proliferation and differentiation during skeletal muscle myogenesis contribute to the physiological function of muscles. Certain non-coding RNAs, including long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs), are involved in the regulation of muscle development, and the aberrant expressions of lncRNAs and circRNAs are associated with muscular diseases. In this review, we summarize the recent advances concerning the roles of lncRNAs and circRNAs in regulating the developmental aspects of myogenesis. These findings have remarkably broadened our understanding of the gene regulation mechanisms governing muscle proliferation and differentiation, which makes it more feasible to design novel preventive, diagnostic and therapeutic strategies for muscle disorders. K E Y W O R D ScircRNAs, development, differentiation, lncRNAs, proliferation, skeletal muscle

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A Zfp609 circular RNA regulates myoblast differentiation by sponging miR-194-5p

Cited by 77 publications

References 34 publications

Circular RNAs in Muscle Function and Disease

Circular RNAs in Muscle Function and Disease

Circular RNA circMYL1 inhibit proliferation and promote differentiation of myoblasts by sponging miR-2400

Roles of lncRNAs and circRNAs in regulating skeletal muscle development

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