A zeta potential value determines the aggregate’s size of penta-substituted [60]fullerene derivatives in aqueous suspension whereas positive charge is required for toxicity against bacterial cells

Deryabin, D. G.; Efremova, Ludmila V.; Vasilchenko, Alexey S.; Сайдакова, Е. В.; Sizova, Еlena; Troshin, Pavel A.; Zhilenkov, Alexander V.; Khakina, Ekaterina A.

doi:10.1186/s12951-015-0112-6

Cited by 45 publications

(18 citation statements)

References 35 publications

(34 reference statements)

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“…The relationship between zeta potentials and size of functionalized C 60 aggregates and their influence on the toxicity against bacterial cells was recently described. It was shown, that an increase of surface charge (indifferently, + or −) always leads to a decrease in the size of nanoparticles, but the toxicity is always associated with positively charged C 60 aggregates [ 48 ]. Nanoparticles in the nC 60 always bear a negative charge.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory effect of fullerene C60 in a mice model of atopic dermatitis

et al. 2016

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Background Water-soluble form of fullerene C60 is a promising tool for the control of ROS-dependent inflammation including allergic diseases. Anti-inflammatory effects of C60 (nC60) aqueous dispersion were evaluated in the mouse models of atopic dermatitis using subcutaneous (SC) and epicutaneous (EC) applications during 50 days period. A highly stable nC60 was prepared by exhaustive dialysis of water-organic C60 solution against water, where the size and ζ-potential of fullerene nanoparticles are about 100 nm and −30 mV, respectively.ResultsTo induce skin inflammation, female BALB/c mice were EC sensitized with ovalbumin three times during one-weekly exposures. The nC60 solution was administrated in mice subcutaneously (SC) (0.1 mg/kg) and epicutaneously (EC) (1 mg/kg). Significant suppression of IgE and Th2 cytokines production and a concomitant rise in concentrations of Th1 cytokines were observed in nC60-treated groups. In addition, a significant increase in the levels of Foxp3+ and filaggrin mRNA expression was observed at EC application. Histological examination of skin samples indicated that therapeutic effect was achieved by both EC and SC treatment, but it was more effective with EC. Pronounced reduction of the eosinophil and leukocyte infiltration in treated skin samples was observed.ConclusionsWe suppose that nC60 treatment shifts immune response from Th2 to Th1 and restores to some extent the function of the skin barrier. This approach can be a good alternative to the treatment of allergic and other inflammatory diseases.

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Section: Discussionmentioning

confidence: 99%

Anti-inflammatory effect of fullerene C60 in a mice model of atopic dermatitis

et al. 2016

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“…The electrophoretic mobility gives the charges which has an impact on the particle distribution (Deryabin et al, 2015). The higher the zeta potential value the higher the electric charge on the surface of the particles.…”

Section: Zeta Potentialmentioning

confidence: 99%

Bacillus velezensis RC 218 as a biocontrol agent to reduce Fusarium head blight and deoxynivalenol accumulation: Genome sequencing and secondary metabolite cluster profiles

Palazzini

Dunlap

Bowman

et al. 2016

Microbiological Research

166

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Bacillus subtilis RC 218 was originally isolated from wheat anthers as a potential antagonist of Fusarium graminearum, the causal agent of Fusarium head blight (FHB). It was demonstrated to have antagonist activity against the plant pathogen under in vitro and greenhouse assays. The current study extends characterizing B. subtilis RC 218 with a field study and genome sequencing. The field study demonstrated that B. subtilis RC 218 could reduce disease severity and the associated mycotoxin (deoxynivalenol) accumulation, under field conditions. The genome sequencing allowed us to accurately determine the taxonomy of the strain using a phylogenomic approach, which places it in the Bacillus velezensis clade. In addition, the draft genome allowed us to use bioinformatics to mine the genome for potential metabolites. The genome mining allowed us to identify 9 active secondary metabolites conserved by all B. velezensis strains and one additional secondary metabolite, the lantibiotic ericin, which is unique to this strain. This study represents the first confirmed production of ericin by a B. velezensis strain. The genome also allowed us to do a comparative genomics with its closest relatives and compare the secondary metabolite production of the publically available B. velezensis genomes. The results showed that the diversity in secondary metabolites of strains in the B. velezensis clade is driven by strains making different antibacterials.

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“…These results suggest that fine-tuning of the net charge of the complex is possible and therefore it should also be possible to take advantage of the nature of each protein to create optimal C60-protein systems as a function of the pH. Tuning the net charge of the protein used to host the C60 molecule it is possible to governs its interactions with cellular and bacterial surface, controlling C60 toxicity [60][61][62][63].…”

Section: Stability Of the Complex In Aqueous Mediamentioning

confidence: 99%

C<sub>60</sub> Bioconjugation with Proteins: Towards a Palette of Carriers for All pH Ranges

Giosia¹,

Valle²,

Cantelli³

et al. 2018

Preprint

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The high hydrophobicity of fullerenes and the resulting formation of aggregates in aqueous solutions hamper the possibility of their exploitation in many technological applications. Noncovalent bioconjugation of fullerenes with proteins is an emerging approach for their dispersion in aqueous media. Contrary to covalent functionalization, bioconjugation preserves the physicochemical properties of the carbon nanostructure. The unique photophysical and photochemical properties of fullerenes are then fully accessible for applications in nanomedicine, sensoristic, biocatalysis and materials science fields. And yet, proteins are not universal carriers. Their stability depends on the biological conditions for which they have evolved. Here we present two model systems based on pepsin and trypsin. These proteins have opposite net charge at physiological pH. They recognize and disperse C60 in water. UV-Vis spectroscopy, zeta-potential and atomic force microscopy analysis demonstrates that the hybrids are well dispersed and stable in a wide range of pH’s and ionic strengths. A previously validated modelling approach identifies the protein binding pocket involved in the interaction with C60. Computational predictions, combined with experimental investigations, provide powerful tools to design tailor-made C60@proteins bioconjugates for specific applications.

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A zeta potential value determines the aggregate’s size of penta-substituted [60]fullerene derivatives in aqueous suspension whereas positive charge is required for toxicity against bacterial cells

Cited by 45 publications

References 35 publications

Anti-inflammatory effect of fullerene C60 in a mice model of atopic dermatitis

Anti-inflammatory effect of fullerene C60 in a mice model of atopic dermatitis

Bacillus velezensis RC 218 as a biocontrol agent to reduce Fusarium head blight and deoxynivalenol accumulation: Genome sequencing and secondary metabolite cluster profiles

C<sub>60</sub> Bioconjugation with Proteins: Towards a Palette of Carriers for All pH Ranges

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