A zebrafish model of intrahepatic cholangiocarcinoma by dual expression of hepatitis B virus X and hepatitis C virus core protein in liver

Liu, Wangta; Chen, Jim-Ray; Hsu, Chih‐Hao; Li, Yen-Hsing; Chen, Yi-Meng; Lin, Chien‐Yuan; Huang, Shin-Jie; Chang, Zen-Kuei; Chen, Yen‐Chun; Lin, Chi-Hsueh; Gong, Hong‐Yi; Lin, Chia Feng; Kawakami, Koichi; Wu, Jen‐Leih

doi:10.1002/hep.25914

Cited by 60 publications

(53 citation statements)

References 54 publications

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“…Some recent studies with human viruses also address that cell-and tissuespecific pathologies can be visualized with the zebrafish model. 23,[38][39][40][41] The future goal is to complement what we have learned in the mouse model and use genetic and embryological benefits of the zebrafish system to address human viral infections. There are multiple strengths in the zebrafish model, which can bring virologists, immunologists, and cell biologists together.…”

Section: Discussionmentioning

confidence: 99%

“…21 In a separate study, Liu et al further demonstrated a unique example of viral tropism in an adult zebrafish model. 23 To learn the mechanism of intrahepatic cholangiocarcinoma (ICC) associated with hepatitis B and hepatitis C viruses (HCV), a conditional expression system with the hepatitis B virus X protein and HCV core protein was generated in the liver of zebrafish, which showed signs of liver fibrosis and development of ICC. 23 Interestingly, the profiles of biomarkers in zebrafish ICC were similar to those of human ICC.…”

Section: Viral Tropism In Zebrafish Modelmentioning

confidence: 99%

“…23 To learn the mechanism of intrahepatic cholangiocarcinoma (ICC) associated with hepatitis B and hepatitis C viruses (HCV), a conditional expression system with the hepatitis B virus X protein and HCV core protein was generated in the liver of zebrafish, which showed signs of liver fibrosis and development of ICC. 23 Interestingly, the profiles of biomarkers in zebrafish ICC were similar to those of human ICC. In addition, the study further demonstrated a loss of ICC formation in the presence of doxycycline treatment.…”

Section: Viral Tropism In Zebrafish Modelmentioning

confidence: 99%

“…In addition, the study further demonstrated a loss of ICC formation in the presence of doxycycline treatment. 23 Ding et al, generated a zebrafish larvae model expressing NS5B that amplified the HCV replicon showing expression of HCV core RNA and protein. 24 When ribavirin and oxymatrine, two known anti-HCV drugs were used, the amplification of HCV replicon was inhibited suggesting the use of zebrafish larvae model for screening and evaluation of anti-HCV drugs.…”

Section: Viral Tropism In Zebrafish Modelmentioning

confidence: 99%

“…30,31,34,35 Although only few human viruses 21,[23][24][25][26][36][37][38][39] have been reported to infect zebrafish, the potential to take advantage of the zebrafish model is growing. There are several advantages of utilizing zebrafish to use microbial infections such as its small size, rapid breeding cycle, low maintenance cost offers large-scale infection analysis that can be very useful in defining infection requirements and confirming them in previously defined mammalian models 40,41 ( Table 3).…”

Section: Zebrafish Model: Overall Advantages To Study Viral Infectionsmentioning

confidence: 99%

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Zebrafish: Modeling for Herpes Simplex Virus Infections

et al. 2014

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For many years, zebrafish have been the prototypical model for studies in developmental biology. In recent years, zebrafish has emerged as a powerful model system to study infectious diseases, including viral infections. Experiments conducted with herpes simplex virus type-1 in adult zebrafish or in embryo models are encouraging as they establish proof of concept with viral-host tropism and possible screening of antiviral compounds. In addition, the presence of human homologs of viral entry receptors in zebrafish such as 3-O sulfated heparan sulfate, nectins, and tumor necrosis factor receptor superfamily member 14-like receptor bring strong rationale for virologists to test their in vivo significance in viral entry in a zebrafish model and compare the structure-function basis of virus zebrafish receptor interaction for viral entry. On the other end, a zebrafish model is already being used for studying inflammation and angiogenesis, with or without genetic manipulations, and therefore can be exploited to study viral infection-associated pathologies. The major advantage with zebrafish is low cost, easy breeding and maintenance, rapid lifecycle, and a transparent nature, which allows visualizing dissemination of fluorescently labeled virus infection in real time either at a localized region or the whole body. Further, the availability of multiple transgenic lines that express fluorescently tagged immune cells for in vivo imaging of virus infected animals is extremely attractive. In addition, a fully developed immune system and potential for receptor-specific knockouts further advocate the use of zebrafish as a new tool to study viral infections. In this review, we focus on expanding the potential of zebrafish model system in understanding human infectious diseases and future benefits.

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Section: Discussionmentioning

confidence: 99%

Section: Viral Tropism In Zebrafish Modelmentioning

confidence: 99%

Section: Viral Tropism In Zebrafish Modelmentioning

confidence: 99%

Section: Viral Tropism In Zebrafish Modelmentioning

confidence: 99%

Section: Zebrafish Model: Overall Advantages To Study Viral Infectionsmentioning

confidence: 99%

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Zebrafish: Modeling for Herpes Simplex Virus Infections

et al. 2014

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Zebrafish Models of Human Liver Development and Disease

Wilkins

Pack

2013

Comprehensive Physiology

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The liver performs a large number of essential synthetic and regulatory functions that are acquired during fetal development and persist throughout life. Their disruption underlies a diverse group of heritable and acquired diseases that affect both pediatric and adult patients. Although experimental analyses used to study liver development and disease are typically performed in cell culture models or rodents, the zebrafish is increasingly used to complement discoveries made in these systems. Forward and reverse genetic analyses over the past two decades have shown that the molecular program for liver development is largely conserved between zebrafish and mammals, and that the zebrafish can be used to model heritable human liver disorders. Recent work has demonstrated that zebrafish can also be used to study the mechanistic basis of acquired liver diseases. Here, we provide a comprehensive summary of how the zebrafish has contributed to our understanding of human liver development and disease.

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Important roles played by TGF‐β in hepatitis B infection

Karimi-Googheri

Daneshvar

Nosratabadi

et al. 2013

Journal of Medical Virology

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Hepatitis B virus (HBV) which includes, fulminant, acute, chronic, asymptomatic, and occult HBV infection is the most prevalent virus that leads to human liver diseases. Chronic, asymptomatic, and occult infection can induce further sever diseases such as hepatocellular carcinoma (HCC) and cirrhosis of the liver. The underlying mechanisms that allow progression of the prolonged forms of the infection and subsequent HCC or cirrhosis of the liver are yet to be clarified. However, many researchers have suggested that immunological and genetic parameters may play important roles in the etiology of hepatitis B. Transforming growth factor beta (TGF-β) is an important cytokine with dual regulatory functions in the immune system and in the responses against viral infections. However, the pathways and mechanisms controlling these are not fully understood. The crucial roles of TGF-β in the development of Th17 and T regulatory lymphocytes, the main cell types involved in autoimmunity and destructive immune related diseases, have been documented and this provides insights into TGF-β function during hepatitis infection and subsequent HCC and cirrhosis of the liver. Recent findings also confirm that TGF-β directly alters hepatocyte function during hepatitis B, hence, the aim of this review is to address the current data regarding the association and status of TGF-β with hepatitis B infection and its related disorders including HCC and cirrhosis of the liver.

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A zebrafish model of intrahepatic cholangiocarcinoma by dual expression of hepatitis B virus X and hepatitis C virus core protein in liver

Cited by 60 publications

References 54 publications

Zebrafish: Modeling for Herpes Simplex Virus Infections

Zebrafish: Modeling for Herpes Simplex Virus Infections

Zebrafish Models of Human Liver Development and Disease

Important roles played by TGF‐β in hepatitis B infection

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