A yeast protein with homology to the β-subunit of G proteins is involved in control of heme-regulated and catabolite-repressed genes

Zhang, Ming; Rosenblum-Vos, Lynne S.; Lowry, Charles V.; Boakye, Ken; Zitomer, R S

doi:10.1016/0378-1119(91)90047-f

Cited by 68 publications

(60 citation statements)

References 34 publications

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“…Thus, base pairs 432-1907 of SSN6 were replaced with pRS406 sequences. TUP1 was disrupted in a similar fashion, by use of fragments corresponding to base pairs 86-254 and 1753-1964 of the TUP1 sequence (with base pair 1 denoting the A residue of the translational start site; Williams and Trumbly 1991;Zhang et al 1991), resulting in replacement of base pairs 254--1964 of the TUPI gene with pRS406 sequences. Both ssn6 and tupl mutants displayed clumpy phenotypes; in addition, Cold Spring Harbor Laboratory Press on May 10, 2018 -Published by genesdev.cshlp.org Downloaded from both disruptions were verified by genomic Southern blotting (Southern 1975).…”

Section: Disruption Of the Ssn6 And Tup1 Genesmentioning

confidence: 99%

The global transcriptional regulators, SSN6 and TUP1, play distinct roles in the establishment of a repressive chromatin structure.

Cooper¹,

Roth²,

Simpson³

1994

Genes Dev.

184

161

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Repression of a-cell specific gene expression in yeast ~ cells requires MATch2 and MCM1, as well as two global repressors, SSN6 and TUP1. Previous studies demonstrated that nucleosomes positioned adjacent to the a2/MCM1 operator in c~ cells directly contribute to repression. To investigate the possibility that SSN6 and TUP1 provide a link between MAT~2/MCM1 and neighboring histones, nucleosome locations were examined in ssn6 and t u p l ot cells. In both cases, nucleosome positions downstream of the operator were disrupted, and the severity of the disruption correlated with the degree of derepression. Nevertheless, the observed changes in chromatin structure were not dependent on transcription. Our data strongly indicate that SSN6 and TUP1 directly organize repressive regions of chromatin.

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Section: Disruption Of the Ssn6 And Tup1 Genesmentioning

confidence: 99%

The global transcriptional regulators, SSN6 and TUP1, play distinct roles in the establishment of a repressive chromatin structure.

Cooper¹,

Roth²,

Simpson³

1994

Genes Dev.

184

161

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“…In addition, mutations in two of the abovementioned genes, SSN6 and TUPI, were identified during hunts for mutants with increased expression of the CYC7 gene (3,32,52). In the case of tup) mutations, overexpression appears to be in part due to the lack of activity of the ROX1 repressor (52). We have continued the search for genes involved in CYC7 regulation and report here the identification of a gene encoding an essential nuclear protein.…”

mentioning

confidence: 94%

“…The mechanisms of catabolite repression are not well characterized, although some of the components have been identified. The expression of both cytochrome c protein genes and the COX6 gene, encoding a subunit of cytochrome oxidase, is affected by mutations in the SNFJ (COX6 and CYCJ) (48), SSN6/CYC8 (CYCI, CYC7, and COX6) (32, 48), and TUPJICYC9 (CYCI and CYC7) (32,52) genes, which also regulate the expression of the catabolite-repressed invertase and the galactose catabolic enzymes (36,43,47), suggesting a common link between catabolite repression among these systems. However, these regulatory genes seem to play a much more general role in regulating cellular functions.…”

mentioning

confidence: 99%

“…However, these regulatory genes seem to play a much more general role in regulating cellular functions. Mutations in SSN6 and TUPI render MATt cells sterile (14,23,34), and mutations in TUPI cause derepression of heme-repressed and heme-activated genes (52). Thus, these genes may be involved either in circuitry which processes the input from multiple signals and transmits them to the appropriate DNA-binding proteins or in mediating interactions between specific repressors and the general transcriptional machinery.…”

mentioning

confidence: 99%

“…Thus, mutations in the HAP] gene that generate a protein with altered specificity and null mutations in ROX1 were, in hindsight predictably, isolated by causing CYC7 overexpression (18,45). In addition, mutations in two of the abovementioned genes, SSN6 and TUPI, were identified during hunts for mutants with increased expression of the CYC7 gene (3,32,52). In the case of tup) mutations, overexpression appears to be in part due to the lack of activity of the ROX1 repressor (52).…”

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confidence: 99%

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The ROX3 gene encodes an essential nuclear protein involved in CYC7 gene expression in Saccharomyces cerevisiae.

et al. 1991

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The ROX3 gene was identified during a hunt for mutants with increased expression of the heme-regulated CYC7 gene, which encodes the minor species of cytochrome c in the yeast Saccharomyces cerevisiae. The rox3 mutants caused a 10-fold increase in CYC7 expression both in the presence and absence of heme, had slightly increased anaerobic expression of the heme-activated CYCI gene, and caused decreases in the anaerobic expression of the heme-repressed ANBI gene and the aerobic expression of its heme-induced homolog. The wild-type ROX3 gene was cloned, and the sequence indicated that it encodes a 220-amino-acid protein. This protein is essential; deletion of the coding sequence was lethal. The coding sequence for j-galactosidase was fused to the 3' end of the ROX3 coding sequence, and the fusion product was found to be localized in the nucleus, strongly suggesting that the wild-type protein carries out a nuclear function. Mutations in the rox3 gene showed an interesting pattern of intragenic complementation. A deletion of the 5' coding region complemented a nonsense mutation at codon 128 but could not prevent the lethality of the null mutation. These results suggest that the amino-terminal domain is required for an essential function, while the carboxy-terminal domain can be supplied in trans to achieve the wild-type expression of CYC7. Finally, RNA blots demonstrated that the ROX3 mRNA was expressed at higher levels anaerobically but was not subject to heme repression. The nuclear localization and the lack of viability of null mutants suggest that the ROX3 protein is a general regulatory factor.

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Transcriptional co-regulation ofSaccharomyces cerevisiae alcohol acetyltransferase gene,ATF1 and Δ-9 fatty acid desaturase gene,OLE1 by unsaturated fatty acids

Fujiwara

Yoshimoto

Sone

et al. 1998

Yeast

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The ATF1 gene encodes an alcohol acetyl transferase which catalyzes the synthesis of acetate esters from acetyl CoA and several kinds of alcohols. ATF1 expression is repressed by unsaturated fatty acids or oxygen. Analysis using ATF1‐lacZ fusion plasmid revealed that ATF1 gene expression is widely repressed by a variety of unsaturated fatty acids, and the degree of ATF1 transcriptional repression varies according to the structure of the unsaturated fatty acids. Interestingly, it was noted that the degree of ATF1 transcriptional repression was related to the melting point of unsaturated fatty acids added to the medium. The OLE1 gene, which encodes Δ‐9 fatty acid desaturase, has been reported to be repressed by unsaturated fatty acids. Transcription of OLE1 was also repressed by a wide variety of unsaturated fatty acids under anaerobic conditions. The degree of transcriptional repression of OLE1 was also related to the melting point of the added unsaturated fatty acids. Therefore, it is considered that ATF1 and OLE1 transcription are regulated in response to cell membrane fluidity. As has been reported for OLE1, the repression of ATF1 by unsaturated fatty acids was relieved in a disruptant carrying a faa1 and faa4 double mutation, two fatty acid activation genes. However, the ATF1 transcript in this double gene disruptant was repressed by oxygen. These results suggested that ATF1 transcription was co‐regulated by the same mechanism as the OLE1 gene and that unsaturated fatty acids and oxygen repressed the ATF1 transcript by a different regulation pathway. © 1998 John Wiley & Sons, Ltd.

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A yeast protein with homology to the β-subunit of G proteins is involved in control of heme-regulated and catabolite-repressed genes

Cited by 68 publications

References 34 publications

The global transcriptional regulators, SSN6 and TUP1, play distinct roles in the establishment of a repressive chromatin structure.

The global transcriptional regulators, SSN6 and TUP1, play distinct roles in the establishment of a repressive chromatin structure.

The ROX3 gene encodes an essential nuclear protein involved in CYC7 gene expression in Saccharomyces cerevisiae.

Transcriptional co-regulation ofSaccharomyces cerevisiae alcohol acetyltransferase gene,ATF1 and Δ-9 fatty acid desaturase gene,OLE1 by unsaturated fatty acids

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