A yeast H2A-H2B promoter can be regulated by changes in histone gene copy number.

Moran, Laurie S.; Norris, David; Osley, Mary Ann

doi:10.1101/gad.4.5.752

Cited by 56 publications

(74 citation statements)

References 44 publications

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“…Furthermore, dosage compensation of HTA1-HTB1 requires histone production because a frameshift mutation introduced into HTB1 eliminates dosage compensation. This very interesting observation suggests that a feedback system might simultaneously involve the NEG system, the 39-UTR, and histones (Moran et al 1990). …”

Section: Histone Genes and Dosage Compensationmentioning

confidence: 93%

“…Dosage compensation of the HTA1-HTB1 locus also involves the NEG system (Moran et al 1990). Transcription of an HTA1 reporter gene can be repressed or activated, depending on the number of HTA and HTB genes in the cell, whereas an HTA2 reporter gene is unaffected.…”

Section: Histone Genes and Dosage Compensationmentioning

confidence: 99%

“…Outside S phase, the activators are displaced or degraded (arrows). Our hypothesis is based particularly on our own work and that of Moran et al (1990) and Fillingham et al (2009). There are additional levels of control at the levels of mRNA stability and Rad53-mediated histone degradation (not shown).…”

Section: Hypothesis: Negative Feedback Links the Uas And Neg Systemsmentioning

confidence: 99%

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Regulation of Histone Gene Expression in Budding Yeast

Eriksson¹,

Ganguli²,

Nagarajavel³

et al. 2012

Genetics

116

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We discuss the regulation of the histone genes of the budding yeast Saccharomyces cerevisiae. These include genes encoding the major core histones (H3, H4, H2A, and H2B), histone H1 (HHO1), H2AZ (HTZ1), and centromeric H3 (CSE4). Histone production is regulated during the cell cycle because the cell must replicate both its DNA during S phase and its chromatin. Consequently, the histone genes are activated in late G1 to provide sufficient core histones to assemble the replicated genome into chromatin. The major core histone genes are subject to both positive and negative regulation. The primary control system is positive, mediated by the histone gene-specific transcription activator, Spt10, through the histone upstream activating sequences (UAS) elements, with help from the major G1/S-phase activators, SBF (Swi4 cell cycle box binding factor) and perhaps MBF (MluI cell cycle box binding factor). Spt10 binds specifically to the histone UAS elements and contains a putative histone acetyltransferase domain. The negative system involves negative regulatory elements in the histone promoters, the RSC chromatin-remodeling complex, various histone chaperones [the histone regulatory (HIR) complex, Asf1, and Rtt106], and putative sequence-specific factors. The SWI/SNF chromatin-remodeling complex links the positive and negative systems. We propose that the negative system is a damping system that modulates the amount of transcription activated by Spt10 and SBF. We hypothesize that the negative system mediates negative feedback on the histone genes by histone proteins through the level of saturation of histone chaperones with histone. Thus, the negative system could communicate the degree of nucleosome assembly during DNA replication and the need to shut down the activating system under replication-stress conditions. We also discuss post-transcriptional regulation and dosage compensation of the histone genes.

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Section: Histone Genes and Dosage Compensationmentioning

confidence: 93%

Section: Histone Genes and Dosage Compensationmentioning

confidence: 99%

Section: Hypothesis: Negative Feedback Links the Uas And Neg Systemsmentioning

confidence: 99%

See 1 more Smart Citation

Regulation of Histone Gene Expression in Budding Yeast

Eriksson¹,

Ganguli²,

Nagarajavel³

et al. 2012

Genetics

116

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show abstract

“…Interestingly, many of these factors (Hir, Hpc, Asf1, Rtt106) also function as histone chaperones, described in the section on histone chaperones, strongly suggesting that histone gene transcription is regulated by free histone levels. There are also post-transcriptional mechanisms that control histone levels in yeast, including dosage compensation (Moran et al 1990), gene amplification (Libuda and Winston 2006), and protein stability (Gunjan and Verreault 2003;Singh et al 2009;Morillo-Huesca et al 2010b). …”

Section: Altering Histone Levels Changes Transcription In Vivomentioning

confidence: 99%

Chromatin and Transcription in Yeast

2012

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Understanding the mechanisms by which chromatin structure controls eukaryotic transcription has been an intense area of investigation for the past 25 years. Many of the key discoveries that created the foundation for this field came from studies of Saccharomyces cerevisiae, including the discovery of the role of chromatin in transcriptional silencing, as well as the discovery of chromatin-remodeling factors and histone modification activities. Since that time, studies in yeast have continued to contribute in leading ways. This review article summarizes the large body of yeast studies in this field.

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“…As histone H2A and H2B protein levels may potentially regulate the HTA1/ HTB1 promoter through a feedback mechanism (Moran et al 1990), the introduced plasmids did not contain the intact protein-coding sequences. We distinguished the endogenous HTA1/HTB1 locus from the plasmid harboring the deletion alleles by using primers that yield PCR products of two different sizes.…”

Section: Specific Recruitment Of Rsc To the Hta1 Promoter Depends On mentioning

confidence: 99%

Genome-wide location and regulated recruitment of the RSC nucleosome-remodeling complex

Ng¹,

Robert²,

Young³

et al. 2002

Genes Dev.

254

292

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Genome-wide location analysis indicates that the yeast nucleosome-remodeling complex RSC has ∼700 physiological targets and that the Rsc1 and Rsc2 isoforms of the complex behave indistinguishably. RSC is associated with numerous tRNA promoters, suggesting that the complex is recruited by the RNA polymerase III transcription machinery. At RNA polymerase II promoters, RSC specifically targets several gene classes, including histones, small nucleolar RNAs, the nitrogen discrimination pathway, nonfermentative carbohydrate metabolism, and mitochondrial function. At the histone HTA1/HTB1 promoter, RSC recruitment requires the Hir1 and Hir2 corepressors, and it is associated with transcriptional inactivity. In contrast, RSC binds to promoters involved in carbohydrate metabolism in response to transcriptional activation, but prior to association of the Pol II machinery. Therefore, the RSC complex is generally recruited to Pol III promoters and it is specifically recruited to Pol II promoters by transcriptional activators and repressors.

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A yeast H2A-H2B promoter can be regulated by changes in histone gene copy number.

Cited by 56 publications

References 44 publications

Regulation of Histone Gene Expression in Budding Yeast

Regulation of Histone Gene Expression in Budding Yeast

Chromatin and Transcription in Yeast

Genome-wide location and regulated recruitment of the RSC nucleosome-remodeling complex

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