A Yeast Capable of Utilizing Methanol

Ogata, Kôichi; NISHIKAWA, Hideo; Ohsugi, Masahiro

doi:10.1271/bbb1961.33.1519

Cited by 79 publications

(24 citation statements)

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“…Methylotrophs are microorganisms that use methanol as their sole carbon and energy source, and methylotrophic yeasts are the only eukaryotic cells with this type of metabolism (Ogata et al, 1969). The methylotrophic yeasts have been studied for years, are the subject of both basic and applied research, including understanding methanol-metabolic pathways , for use as expression systems with strong methanolinducible promoters (Raymond et al, 1998;Gellissen, 2000;Houard et al, 2002;Nakagawa et al 2006), for understanding peroxisome biogenesis and degradation (Lazarow, 2003;Farre and Subramani, 2004), yeast prions (Kushnirov et al, 2000) and fatty acid production (Lu et al, 2000;Aoki et al, 2002), and for development as biosensors (Reshetilov et al, 2001).…”

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confidence: 99%

Distribution, diversity and regulation of alcohol oxidase isozymes, and phylogenetic relationships of methylotrophic yeasts

Ito

Fujimura

Uchino

et al. 2007

Yeast

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In this study, we attempted to classify the methylotrophic yeasts based on diversities of alcohol oxidase (AOD), i.e. zymogram patterns and partial amino acid sequences. According to zymogram patterns for AOD, members of the methylotrophic yeasts separate into two major lineages, one group involving strains having a single AOD and the other group, including Pichia methanolica, Candida pignaliae and C. sonorensis, showing nine AOD isozymes. Based on partial amino acid sequences of AOD, the methylotrophic yeasts could be divided into five groups, and this classification agrees mostly with grouping based on 26S domain D1/D2 rDNA nucleotide sequences, except for some strains. Moreover, the strains having AOD isozymes constitute one group with P. trehalophila, P. glucozyma and Pichia sp. strain BZ159, although these strains are divided into two types, based on amino acid sequences of second AODs. On the other hand, these AOD isozymes consist of two subunits; the first subunits are induced not only by methanol but also by glycerol and pectin, although the second subunits are mainly induced by methanol. These data indicate that AOD isozymes and second AOD genes distribute widely in several methylotrophic yeasts in the natural environment, and second AOD genes may have evolved as methylotrophic genes that can adapt to the environmental conditions of higher methanol concentrations.

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Section: Introductionmentioning

confidence: 99%

Distribution, diversity and regulation of alcohol oxidase isozymes, and phylogenetic relationships of methylotrophic yeasts

Ito

Fujimura

Uchino

et al. 2007

Yeast

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Since the fi rst report of methanol-assimilating yeast by Ogata et al (1969), a number of methanol-assimilating yeast species were reported. The majority of these yeast species are distributed into three clades, the Ogataea, the Kuraishia and the Komagataella, in the phylogenetic trees constructed from nucleic acid sequences (Péter et al, 2008a).

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Candida rishirensis sp. nov., a novel methylotrophic anamorphic yeast species isolated from soil on Rishiri Island in Japan

Nakase

Imanishi

Ninomiya

et al. 2010

J. Gen. Appl. Microbiol.

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Since the fi rst report of methanol-assimilating yeast by Ogata et al. (1969), a number of methanol-assimilating yeast species were reported. The majority of these yeast species are distributed into three clades, the Ogataea, the Kuraishia and the Komagataella, in the phylogenetic trees constructed from nucleic acid sequences (Péter et al., 2008a). In addition, all of the anamorphic methylotrophic yeast species are now placed in the genus Candida, most of which are closely related to the three teleomorphic genera mentioned above. Among these Candida species, the phylogenetic position of Candida pignaliae is not clear. Nagatsuka et al. (2008) reported that all of described species of the genus Ogataea excluding Ogataea nitratoversa (Péter et al., 2008b), three Pichia species and those anamorphic Candida species formed a cluster, the Ogataea cluster, in neighbor-joining and maximum parsimony trees based on the D1/D2 sequences of large-subunit (LSU) rRNA genes, and O. nitratoversa was included in the Pichia methanolica cluster. In their tree, C. pignaliae does not show a close relationship to any known species.During the course of a study of biodiversity of yeasts on Rishiri-tou (Rishiri Island), a small island located in the Japan Sea, two methylotrophic yeast strains, Y07-901-4 and Y07-901-7, were isolated from soil in a pine forest on Mt. Pon, in July 2007, by enrichment culture in media consisting of malt extract (5%), glucose (3%), chloramphenicol (100 μg/ml) and tetracycline (100 μg/ml), with pH 3.7. The two strains are closely related to C. pignaliae in the D1/D2 sequences of LSU rRNA genes but clearly separated from this species by phenotypic or genetic properties. They are described as Candida rishirensis sp. nov. in the present paper.Most of the morphological, physiological, and biochemical characteristics were examined by the methods described by Yarrow (1998). The maximum growth temperature was determined in YM broth using metal block baths at 1 C intervals. The ubiquinone system was analyzed according to the method described by Mikata and Yamada (1999). Nuclear DNA was isolated and purifi ed according to Holm et al. (1986). The DNA base composition was determined by HPLC after enzymatic digestion of DNA to deoxyribonucleosides as described by Tamaoka and Komagata (1984) using a DNA-GC Kit (Yamasa Shoyu Co., Ltd., Chiba, Japan).J. Gen. Appl. Microbiol., 56, 169 173 (2010) Key Words-Candida rishirensis sp. nov.; new yeast from Rishiri Island; novel methylotrophic yeast

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“…Since first isolation in 1969, 12) methylotrophic yeasts have been studied intensively in terms of both physiological activities and potential applications. All methylotrophic yeasts were revealed to have a common methanol-utilizing pathway.…”

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Molecular Basis of Methanol-Inducible Gene Expression and Its Application in the Methylotrophic YeastCandida boidinii

Yurimoto

2009

Bioscience, Biotechnology, and Biochemistry

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Methanol is a promising feedstock for biotechnological and chemical processes as well as a primary source of energy to replace coal and petroleum. Methylotrophic yeasts, that can utilize methanol as the sole source of carbon and energy, have been studied intensively in terms of both physiological activities and potential applications. During growth on methanol, the enzymes involved in methanol metabolism are massively produced in these yeasts, indicating that the gene promoters of these enzymes are strong methanol-inducible promoters. Using these promoters, high-level heterologous gene expression systems have been developed in several methylotrophic yeast strains, such as Pichia pastoris, Hansenula polymorpha, and Candida boidinii. To achieve efficient industrial use of methanol and efficient protein production by methylotrophic yeasts, it is important to elucidate the molecular basis of methanolinducible gene expression in these yeasts. This review describes recent advances in understanding of the regulation of methanol-inducible gene expression and the molecular mechanism of transcriptional activation in the methylotrophic yeast C. boidinii. Application of this gained knowledge led to successful production of useful enzymes in this yeast, which is also reviewed.

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A Yeast Capable of Utilizing Methanol

Cited by 79 publications

References 0 publications

Distribution, diversity and regulation of alcohol oxidase isozymes, and phylogenetic relationships of methylotrophic yeasts

Distribution, diversity and regulation of alcohol oxidase isozymes, and phylogenetic relationships of methylotrophic yeasts

Candida rishirensis sp. nov., a novel methylotrophic anamorphic yeast species isolated from soil on Rishiri Island in Japan

Molecular Basis of Methanol-Inducible Gene Expression and Its Application in the Methylotrophic YeastCandida boidinii

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