A xylem‐produced peptide PtrCLE20 inhibits vascular cambium activity in <i>Populus</i>

Zhu, Yingying; Song, Dongli; Zhang, Rui; Luo, Laifu; Cao, Shun‐an; Huang, Cheng; Sun, Jing; Gui, Jinshan; Li, Laigeng

doi:10.1111/pbi.13187

Cited by 30 publications

(32 citation statements)

References 64 publications

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“…CLE9/ CLE10-HAESA-LIKE1 (HSL1)-SOMATIC EMBRYO-GENESIS RECEPTOR KINASEs (SERKs) regulate stomatal lineage cell division [27]. CLE20 inhibits root growth and lateral root growth by inhibiting RAM and CAM activity, respectively [28,29]. CLE25-BAM induces stomatal closure and promotes drought tolerance by controlling abscisic acid accumulation.…”

mentioning

confidence: 99%

Predicting and clustering plant CLE genes with a new method developed specifically for short amino acid sequences

et al. 2020

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Background The CLV3/ESR-RELATED (CLE) gene family encodes small secreted peptides (SSPs) and plays vital roles in plant growth and development by promoting cell-to-cell communication. The prediction and classification of CLE genes is challenging because of their low sequence similarity. Results We developed a machine learning-aided method for predicting CLE genes by using a CLE motif-specific residual score matrix and a novel clustering method based on the Euclidean distance of 12 amino acid residues from the CLE motif in a site-weight dependent manner. In total, 2156 CLE candidates—including 627 novel candidates—were predicted from 69 plant species. The results from our CLE motif-based clustering are consistent with previous reports using the entire pre-propeptide. Characterization of CLE candidates provided systematic statistics on protein lengths, signal peptides, relative motif positions, amino acid compositions of different parts of the CLE precursor proteins, and decisive factors of CLE prediction. The approach taken here provides information on the evolution of the CLE gene family and provides evidence that the CLE and IDA/IDL genes share a common ancestor. Conclusions Our new approach is applicable to SSPs or other proteins with short conserved domains and hence, provides a useful tool for gene prediction, classification and evolutionary analysis.

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mentioning

confidence: 99%

Predicting and clustering plant CLE genes with a new method developed specifically for short amino acid sequences

et al. 2020

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“…for PtCLE47 gene is Potri.017G074600.1 (Han et al, 2016). It was also annotated as PtrCLE25C (Zhu et al, 2019). Peptide sequences for PtCLE47 and AtCLE25 (AT3G28455) were obtained from Zhu et al (2019).…”

Section: Cloning and Transformation Of Hybrid Aspenmentioning

confidence: 99%

“…Finally, it appears that there are many CLE peptides that affect cambial activity from different sides of the cambium, including PttCLE41 on the phloem side (Etchells et al, 2015;Kucukoglu et al, 2017), PttCLE20 on the xylem side (Zhu et al, 2019), and PttCLE47 in the cambial zone itself (Fig. 6).…”

Section: Researchmentioning

confidence: 99%

“…Reports suggested that the TDIF/CLE41/CLE44‐PXY/TDR signalling module is evolutionarily conserved in regulating the secondary growth and wood formation of Populus trees (Etchells et al , ; Kucukoglu et al , ). Aside from TDIF/CLE41/CLE44‐like genes, a recent study showed that the xylem‐produced peptide PtrCLE20 negatively regulates cambial activity in Populus (Zhu et al , ). Poplars are deciduous, hardwood trees and widely cultivated world‐wide as bioenergy feed stocks and for production of pulp, paper, packing, and woody material.…”

Section: Introductionmentioning

confidence: 99%

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Peptide encoding Populus CLV3/ESR‐RELATED 47 (PttCLE47) promotes cambial development and secondary xylem formation in hybrid aspen

et al. 2019

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Summary The CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION (ESR)‐RELATED (CLE) peptide ligands in connection with their receptors are important players in cell‐to‐cell communications in plants. Here, we investigated the function of the Populus CLV3/ESR‐RELATED 47 (PttCLE47) gene during secondary growth and wood formation in hybrid aspen (Populus tremula × tremuloides) using an RNA interference (RNAi) approach. Expression of PttCLE47 peaks in the vascular cambium. Silencing of the PttCLE47 gene expression affected lateral expansion of stems and decreased apical height growth and leaf size. In particular, PttCLE47 RNAi trees exhibited a narrower secondary xylem zone with less xylem cells/cell file. The reduced radial growth phenotype also correlated with a reduced number of cambial cell layers. In agreement with these results, expression of several cambial regulator genes was downregulated in the stems of the transgenic trees in comparison with controls. Altogether, these results suggest that the PttCLE47 gene is a major positive regulator of cambial activity in hybrid aspen, mainly promoting the production of secondary xylem. Furthermore, in contrast to previously characterized CLE genes expressed in the wood‐forming zone, PttCLE47 appears to be active at its site of expression.

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“…CLE9/CLE10-HAESA-LIKE1 (HSL1)-SOMATIC EMBRYOGENESIS RECEPTOR KINASEs (SERKs) regulate stomatal lineage cell division [27]. CLE20 inhibits root growth and lateral root growth by inhibiting RAM and CAM activity, respectively [28,29]. CLE25-BAM induces stomatal closure and promotes drought tolerance by controlling abscisic acid accumulation.…”

Section: Introductionmentioning

confidence: 99%

Revisiting the Plant CLE Gene Family with a New Method for Predicting and Clustering Short Amino Acid Sequences

Zhang

Liu

Kücükoglu

et al. 2020

Preprint

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Background: The CLV3 / ESR-RELATED ( CLE ) gene family encodes small secreted peptides (SSPs) and plays vital roles in plant growth and development through cell-to-cell communication. The prediction and classification of CLE genes is challenging because of their low sequence similarity. Results: We developed a machine learning-aided method for predicting CLE genes by using a CLE motif-specific residual score matrix and a novel clustering method based on the Euclidean distance of the 12 amino acid residues from CLE motifs in a site-weight dependent manner. In total, 2156 CLE candidates—including 627 novel candidates—were predicted from 69 plant species. The results from our CLE motif-based clustering are consistent with previous reports using the entire pre-propeptide. Characterization of CLE candidates provided systematic statistics on protein lengths, signal peptides, relative motif positions, amino acid compositions of different parts of the CLE precursor proteins, and decisive factors of CLE prediction. The approach taken here provides information on the evolution of the CLE gene family and provides evidence that the CLE and IDA/IDL genes share a common ancestor. Conclusions: Our new approach is applicable to SSPs or other proteins with short conserved domains and hence, provides a useful tool for gene prediction, classification and evolutionary analysis.

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A xylem‐produced peptide PtrCLE20 inhibits vascular cambium activity in Populus

Cited by 30 publications

References 64 publications

Predicting and clustering plant CLE genes with a new method developed specifically for short amino acid sequences

Predicting and clustering plant CLE genes with a new method developed specifically for short amino acid sequences

Peptide encoding Populus CLV3/ESR‐RELATED 47 (PttCLE47) promotes cambial development and secondary xylem formation in hybrid aspen

Revisiting the Plant CLE Gene Family with a New Method for Predicting and Clustering Short Amino Acid Sequences

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