A Xenogeneic-Free Protocol for Isolation and Expansion of Human Adipose Stem Cells for Clinical Uses

Escobedo‐Lucea, Carmen; Bellver, Carmen; Gandía, Carolina; Sanz-García, Andrés; Esteban, Francisco J.; Mirabet, Vicente; Forte, Giancarlo; Moreno, Isabel; Lezameta, Melissa; Ayuso-Sacido, Ángel; García‐Verdugo, José Manuel

doi:10.1371/journal.pone.0067870

Cited by 31 publications

(33 citation statements)

References 43 publications

(56 reference statements)

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“…Fifty-nine full text studies were assessed on eligibility criteria. Fourteen studies were excluded based on the use of a nonintraoperative protocol for isolation as experimental method (Al Battah et al, 2011;Carvalho et al, 2013;Chen et al, 2014;Condé-Green and Lamblet 2012;Doi et al, 2014;Escobedo-Lucea et al, 2013;Fink et al, 2011;Jiang et al, 2007;Okura et al, 2012;Pilgaard et al, 2008;Seaman et al, 2015;Siciliano et al, 2013;Vykoukal et al, 2008;Yoshimura et al, 2006). Seven studies described isolation protocols in general but gave no results (Bernacki et al, 2008;Buehrer and Cheatham 2013;Dubois et al, 2008;Hicok and Hedrick, 2011;Yu et al, 2011;Zachar et al, 2011;Zhu et al, 2013).…”

Section: Included Studiesmentioning

confidence: 99%

Comparison of intraoperative procedures for isolation of clinical grade stromal vascular fraction for regenerative purposes: a systematic review

Dongen

Tuin

Spiekman

et al. 2017

J Tissue Eng Regen Med

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Intraoperative application of the stromal vascular fraction (SVF) of adipose tissue requires a fast and efficient isolation procedure of adipose tissue. This review was performed to systematically assess and compare procedures currently used for the intraoperative isolation of cellular SVF (cSVF) and tissue SVF (tSVF) that still contain the extracellular matrix. Pubmed, EMBASE and the Cochrane central register of controlled trials databases were searched for studies that compare procedures for intraoperative isolation of SVF (searched 28 September 2016). Outcomes of interest were cell yield, viability of cells, composition of SVF, duration, cost and procedure characteristics. Procedures were subdivided into procedures resulting in a cSVF or tSVF. Thirteen out of 3038 studies, evaluating 18 intraoperative isolation procedures, were considered eligible. In general, cSVF and tSVF intraoperative isolation procedures had similar cell yield, cell viability and SVF composition compared to a nonintraoperative (i.e. culture laboratory-based collagenase protocol) control group within the same studies. The majority of intraoperative isolation procedures are less time consuming than nonintraoperative control groups, however. Intraoperative isolation procedures are less time-consuming than nonintraoperative control groups with similar cell yield, viability of cells and composition of SVF, and therefore more suitable for use in the clinic. Nevertheless, none of the intraoperative isolation procedures could be designated as the preferred procedure to isolate SVF.

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Section: Included Studiesmentioning

confidence: 99%

Comparison of intraoperative procedures for isolation of clinical grade stromal vascular fraction for regenerative purposes: a systematic review

Dongen

Tuin

Spiekman

et al. 2017

J Tissue Eng Regen Med

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“…64). Since the behaviour of a particular cell type, such as attachment, growth and the biological responses, can be greatly influenced by specific surface properties, including wettability, electric charge, chemistry, topography and stiffness (Ref.…”

Section: Selection Through Plastic Adherencementioning

confidence: 99%

Critical steps in the isolation and expansion of adipose-derived stem cells for translational therapy

Porsborg

Zachar

Boucher

et al. 2015

Expert Rev. Mol. Med.

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Since the discovery of adipose-derived stem cells (ASCs), there have been high expectations of their putative clinical use. Recent advances support these expectations, and it is expected that the transition from pre-clinical and clinical studies to implementation as a standard treatment modality is imminent. However ASCs must be isolated and expanded according to good manufacturing practice guidelines and a basic assurance of quality, safety, and medical effectiveness is needed for authorisation by regulatory agencies, such as European Medicines Agency and US Food and Drug Administration. In this review, a collection of studies investigating the influence of different steps of the isolation and expansion protocol on the yield and functionality of ASCs has been presented in an attempt to come up with best recommendations that ensure potential beneficial clinical outcome of using ASCs in any therapeutic setting. If the findings confirm the initial observations of beneficial effects of ASCs, the path is paved for implementing these ASC-based therapies as standard treatment options.

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“…211). They have an immunophenotype which is very similar to other MSCs, however, no studies have been performed to test their potential in vivo.…”

Section: Mesenchymal Stem Cells (Mscs) From Bone Marrowmentioning

confidence: 99%

Heart valve tissue engineering: how far is the bedside from the bench?

Sanz-García

Cruz

Mirabet³

et al. 2015

Expert Rev. Mol. Med.

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Heart disease, including valve pathologies, is the leading cause of death worldwide. Despite the progress made thanks to improving transplantation techniques, a perfect valve substitute has not yet been developed: once a diseased valve is replaced with current technologies, the newly implanted valve still needs to be changed some time in the future. This situation is particularly dramatic in the case of children and young adults, because of the necessity of valve growth during the patient's life. Our review focuses on the current status of heart valve (HV) therapy and the challenges that must be solved in the development of new approaches based on tissue engineering. Scientists and physicians have proposed tissue-engineered heart valves (TEHVs) as the most promising solution for HV replacement, especially given that they can help to avoid thrombosis, structural deterioration and xenoinfections. Lastly, TEHVs might also serve as a model for studying human valve development and pathologies.

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A Xenogeneic-Free Protocol for Isolation and Expansion of Human Adipose Stem Cells for Clinical Uses

Cited by 31 publications

References 43 publications

Comparison of intraoperative procedures for isolation of clinical grade stromal vascular fraction for regenerative purposes: a systematic review

Comparison of intraoperative procedures for isolation of clinical grade stromal vascular fraction for regenerative purposes: a systematic review

Critical steps in the isolation and expansion of adipose-derived stem cells for translational therapy

Heart valve tissue engineering: how far is the bedside from the bench?

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